Superfusion in Vitro in the Study of Ovarian Steroidogenesis

Watson, John; Leask, J.T.S.

doi:10.1677/joe.0.0640163

Cited by 23 publications

(13 citation statements)

References 14 publications

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“…Secretion of the steroid from the control tissue was relatively steady over the experi¬ mental period. The presence of LH in the superfusion medium produced, as expected, considerable stimulation of progesterone secretion and the pattern was similar to that noted previously for nonpregnant pigs (Watson & Leask, 1975;Watson & Wrigglesworth, 1975) in that there was a rapid transient increase in progesterone secretion (attributed to release of preformed steroid) followed by a prolonged stimulation of the steroid which lasted throughout the remainder of the experimental period. This response to LH was similar with tissue from all the animals studied and for all the times used (2-4 h).…”

Section: Resultssupporting

confidence: 87%

Progesterone secretion by the corpus luteum of the early pregnant pig during superfusion in vitro with PGF-2 , LH and oestradiol

Watson¹,

Walker²

1978

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Section: Resultssupporting

confidence: 87%

Progesterone secretion by the corpus luteum of the early pregnant pig during superfusion in vitro with PGF-2 , LH and oestradiol

Watson¹,

Walker²

1978

Reproduction

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“…into the superfusion medium. The results of Watson & Leask (1975) are similar in that large quantities of progesterone and small amounts of oestradiol-17/? were produced when slices of porcine luteal tissue were superfused.…”

Section: Discussionmentioning

confidence: 60%

“…When the porcine corpus luteum, in the form of slices or dispersed cells, is superfused in vitro, it secretes progesterone and small quantities of oestradiol-1 ß (Rodway, Dodson & Watson, 1975;Watson & Leask, 1975). In this study we have combined several techniques such that dispersed porcine luteal cells were separated into two populations of cells of dif¬ ferent sizes by sedimentation at unit gravity, and each cell type was superfused separately to define its secretory role.…”

Section: Introductionmentioning

confidence: 99%

Steroid Release in Vitro by Two Luteal Cell Types in the Corpus Luteum of the Pregnant Sow

Lemon¹,

Loir²

1977

Journal of Endocrinology

107

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Corpora lutea from sows at 30, 60 and 90 days of gestation were dissociated enzymically, and the components of the resulting cell suspension were separated by sedimentation at unit gravity. Two luteal cell populations of 30-50 micronm diameter and 15-20 micronm diameter were obtained and superfused for up to 18 h with Dulbecco's modified Eagle medium, the cells being supported in a column in a matrix of Biogel. Fractions were collected every 30 min and assayed for progesterone and oestradiol-17beta. At 30 and 60 days of gestation the large luteal cells produced progesterone at an initial rate of approximately 100 ng/h/10(5) cells, which decreased to half this rate at 90 days. The smaller cells also released progesterone into the medium at approximately 15-20 ng/h/10(5) cells at all stages of gestation. At 30 days of gestation, neither cell type released significant amounts of oestradiol-17beta, but from 60 days onwards ,significant and increasing quantities were measured in the superfusates from the larger cells. Both cell types were perfused with porcine LH at the three stages of gestation, and both showed an immediate response in terms of progesterone release which decreased in magnitude with increasing age of gestation. The response of the smaller cells was greater than that of the larger cells.

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“…In contrast to the bovine CL which seems to lose completely its capacity to synthesize oestradiol-17 ß, human and porcine CL do synthesize oestradiol-17ß (Hammerstein, Rice & Savard, 1964;Watson & Leask, 1975). Preliminary findings, however, utilizing human and porcine ovarian cells in tissue culture, suggest that the aromatase activity of the granulosa cell of these species is much greater than that of luteal cells (K. M. Henderson, Y. S. Moon & .…”

Section: Discussionmentioning

confidence: 99%

Luteinization of bovine granulosa cells and corpus luteum formation associated with loss of androgen-aromatizing ability

Henderson¹,

Moon²

1979

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The relative aromatizing ability of bovine luteinizing granulosa cells and dispersed luteal cells in tissue culture was studied. Luteinization of granulosa cells, as indicated by steadily increasing progesterone production (from 50 to 300 ng/10(5) cells/day over 4--5 days), was accompanied by a dramatic reduction in their capacity to aromatize exogenous androgen; oestradiol-17 beta production falling from 200 to less than 10 ng/10(5) cells/day over 4--5 days. Luteal cells also had only a very limited capacity to aromatize exogenous androgen, maximum oestradiol-17 beta production being less than 600 pg/10(5) cells/day. The loss in aromatizing capacity of granulosa cells during luteinization was also reflected in the relative endogenous steroid content of non-luteinized granulosa cells and luteal tissue, the former containing high levels of oestradiol-17 beta, less than or equal to 28 ng/mg protein, while the latter, although containing substantial amounts of testosterone, less than or equal to 5.7 ng/g tissue, contained very little oestradiol-17 beta, less than or equal to 0.35 ngG TISSUE. These findings suggest that luteinization of bovine granulosa cells and subsequent corpus luteum formation is associated with a loss in androgen aromatase activity.

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Superfusion in Vitro in the Study of Ovarian Steroidogenesis

Cited by 23 publications

References 14 publications

Progesterone secretion by the corpus luteum of the early pregnant pig during superfusion in vitro with PGF-2 , LH and oestradiol

Progesterone secretion by the corpus luteum of the early pregnant pig during superfusion in vitro with PGF-2 , LH and oestradiol

Steroid Release in Vitro by Two Luteal Cell Types in the Corpus Luteum of the Pregnant Sow

Luteinization of bovine granulosa cells and corpus luteum formation associated with loss of androgen-aromatizing ability

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