The second-order scattering study of the Tb(III)–RNA and determination of RNA

Ding, Fen; Zhao, Hang; Xia, Lingyun; Jin, Lin‐Pei

doi:10.1016/j.saa.2005.01.004

Cited by 2 publications

(2 citation statements)

References 43 publications

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“…In 1995, Liu et al (12) first studied SOS as a spectral phenomenon and an analytical technique to determine trace amounts of selenium (13), mercury (14), chromium (15), cadmium (16) and cationic surfactant (17). In recent years, this technique has been increasingly applied to the determination of biological macromolecules such as proteins (18) and nucleic acids (19,20). These studies show that the method has high sensitivity and superior selectivity (13)(14)(15)(16)(17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, this technique has been increasingly applied to the determination of biological macromolecules such as proteins (18) and nucleic acids (19,20). These studies show that the method has high sensitivity and superior selectivity (13)(14)(15)(16)(17)(18)(19)(20). Thus, the SOS technique has potential as a new analytical method with extensive application prospects (21,22).…”

Section: Introductionmentioning

confidence: 99%

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Protein analysis with terbium (III) and sodium dodecyl sulphonate by a second‐order scattering technique

Zhao

Jin

2006

Luminescence

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This is the first report of terbium(III) as a probe of second-order scattering (SOS) for the determination of proteins in human serum at nanogram levels. A sensitive method has been developed using light scattering, based on the fact that the weak SOS of proteins can be enhanced in the presence of terbium(III) and sodium dodecyl sulphonate (SDS). With this method, 7.0 x 10(-9)-1.0 x 10(-5) g/mL human serum albumin (HSA) and 5.0 x 10(-9)-5.0 x 10(-6) g/mL gamma-globulin can be determined; the detection limits were 4.4 ng/mL for HSA and 3.1 ng/mL for gamma-globulin. The method has been applied to the detection of total proteins in human serum samples, and the results are consistent with those obtained by the Coomassie brilliant blue (CBB) G-250 assay.

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