The Seasonality and Ecology of the Anopheles gambiae complex (Dipetra: Culicidae) in Liberia Using Molecular Identification

Fahmy, N. T.; Villinski, Jeffrey T.; Bolay, Fatorma K.; Stoops, Craig A.; Tageldin, R.; Fakoli, Lawrence; Okasha, Omar; Obenauer, P. J.; Diclaro, Joseph W.

doi:10.1093/jme/tjv003

Cited by 7 publications

(3 citation statements)

References 38 publications

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“…gambiae s.s. is the most abundant in the studied locations and the most important within the member of Anopheles gambiae complex. Fahmy et al 16 and Kabbale et al . 17 have described An.…”

Section: Discussionmentioning

confidence: 96%

Molecular identification and wing variations among malaria vectors in Akure North Local Government Area, Nigeria

Akeju

Olusi

Simon-Oke

2022

Sci Rep

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Members of the Anopheles gambiae complex and Anophelesfunestus group are significant vectors of the malaria parasite Plasmodium species in the Afro-tropical region of the world. Molecular identification and variation in the wing were studied among female An.Gambiae complex and An. funestus group, to investigate morphological variations in the wing of local vectors populations of adult female mosquitoes found in five different locations in Akure North Local Government Area of Ondo State (Oba—Ile, Igoba, Isinigbo, Ita—Ogbolu and Iju). The variations in the wing character were found in the 3rd main dark spot area (Pre-apical dark spot—character 8) on the coastal region (Vein region I) of Anopheles gambiae complex wing; with two types (A and B) of wings identified with An. gambiae complex in the study area. Molecular study shows that all the wing type A are Anopheles gambiae s.s., they represent 53.39% of the total An. gambiae complex in the study area. Some of the Anopheles gambiae s.s. (28.30%) and all An. arabiensis (18.30%) were found with wing type B. Among 750 individual Anopheles mosquito species identified using Polymerase Chain Reaction (PCR method), 433 samples representing 57.73% were An. gambiae s.s. while 97 (12.93%) samples were An. arabiensis. Anopheles leesoni was the only member of the An. funestus group identified in the study area. Anopheles leesoni mosquitoes identified in the study location were 182, representing 24.27% of the total Anopheles mosquito species identified using the molecular method. Anopheles gambiae s.s., An. arabiensis, and An. leesoni are only Anopheles mosquito species responsible for malaria transmission in the study area. Anopheles leesoni was the only member of the An. funestus group identified in the study area.

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“…gambiae s.s. is the most abundant in the studied locations and the most important within the member of Anopheles gambiae complex. Fahmy et al 16 and Kabbale et al . 17 have described An.…”

Section: Discussionmentioning

confidence: 96%

Molecular identification and wing variations among malaria vectors in Akure North Local Government Area, Nigeria

Akeju

Olusi

Simon-Oke

2022

Sci Rep

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“…Interestingly, earlier studies in similar ecologies that were conducted during dry periods of the year when temperatures are high alluded to the dominance of An. coluzzii among adult Anopheles mosquitoes [ 45 , 46 ]. Under high temperature conditions, adult An.…”

Section: Discussionmentioning

confidence: 99%

Sibling species of the major malaria vector Anopheles gambiae display divergent preferences for aquatic breeding sites in southern Nigeria

Ebhodaghe,

Sanchez-Vargas,

Isaac

et al. 2024

Malar J

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Background When integrated with insecticide-treated bed nets, larval control of Anopheles mosquitoes could fast-track reductions in the incidence of human malaria. However, larval control interventions may deliver suboptimal outcomes where the preferred breeding places of mosquito vectors are not well known. This study investigated the breeding habitat choices of Anopheles mosquitoes in southern Nigeria. The objective was to identify priority sites for mosquito larval management in selected urban and periurban locations where malaria remains a public health burden. Methods Mosquito larvae were collected in urban and periurban water bodies during the wet-dry season interface in Edo, Delta, and Anambra States. Field-collected larvae were identified based on PCR gel-electrophoresis and amplicon sequencing, while the associations between Anopheles larvae and the properties and locations of water bodies were assessed using a range of statistical methods. Results Mosquito breeding sites were either man-made (72.09%) or natural (27.91%) and mostly drainages (48.84%) and puddles (25.58%). Anopheles larvae occurred in drainages, puddles, stream margins, and a concrete well, and were absent in drums, buckets, car tires, and a water-holding iron pan, all of which contained culicine larvae. Wild-caught Anopheles larvae comprised Anopheles coluzzii (80.51%), Anopheles gambiae sensu stricto (s.s.) (11.54%), and Anopheles arabiensis (7.95%); a species-specific PCR confirmed the absence of the invasive urban malaria vector Anopheles stephensi among field-collected larvae. Anopheles arabiensis, An. coluzzii, and An. gambiae s.s. displayed preferences for turbid, lowland, and partially sunlit water bodies, respectively. Furthermore, An. arabiensis preferred breeding sites located outside 500 m of households, whereas An. gambiae s.s. and An. coluzzii had increased detection odds in sites within 500 m of households. Anopheles gambiae s.s. and An. coluzzii were also more likely to be present in natural water bodies; meanwhile, 96.77% of An. arabiensis were in man-made water bodies. Intraspecific genetic variations were little in the dominant vector An. coluzzii, while breeding habitat choices of populations made no statistically significant contributions to these variations. Conclusion Sibling malaria vectors in the An. gambiae complex display divergent preferences for aquatic breeding habitats in southern Nigeria. The findings are relevant for planning targeted larval control of An. coluzzii whose increasing evolutionary adaptations to urban ecologies are driving the proliferation of the mosquito, and An. arabiensis whose adults typically evade the effects of treated bed nets due to exophilic tendencies.

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“…Many types of molecular techniques have been used to identify insect species, including restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), random amplified polymorphic DNA (RAPD), single-strand conformation polymorphism (SSCP), DNA sequence analysis, and species-specific primer PCR (SS-PCR) methods 9 10 11 12 . Among these techniques, species-specific primer PCR is a popular technology for species identification due to its high accuracy, sensitivity and convenience 13 .…”

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confidence: 99%

The establishment of species-specific primers for the molecular identification of ten stored-product psocids based on ITS2 rDNA

Zhao

Cui

et al. 2016

Sci Rep

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Psocids are important stored product pests found worldwide that can be spread through grain trade. Most stored-product psocids, including eggs, nymphs, and adults, are very small (~1 mm) and difficult to identify morphologically. Here, we collected 10 economically important stored-product Liposcelis spp. psocids (L. bostrychophila, L. entomophila, L. decolor, L. paeta, L. brunnea, L. corrodens, L. mendax, L. rufa, L. pearmani, and L. tricolor) from 35 geographical locations in 5 countries (China, Czech Republic, Denmark, Germany, and the United States). The ITS2 rDNA gene was extracted and sequenced. The interspecific genetic distance of the stored-product psocids was significantly higher than the intraspecific genetic distance according to the barcoding gap analysis. Ten pairs of species-specific primers based on the ITS2 rDNA were developed for psocid identification. The sensitivity estimation indicated that the species-specific primers could correctly amplify the target ITS2 gene and successfully identify psocids at 1.0 ng/mL. Additionally, these species-specific primers could quantify specificity and identify 10 stored-product psocids; this approach could also be used to accurately identify other stored-product psocids. This work provides a practical approach for the precise examination of 10 stored-product psocid species and also contributes to the development of an identification method using ITS2 rDNA.

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The Seasonality and Ecology of the Anopheles gambiae complex (Dipetra: Culicidae) in Liberia Using Molecular Identification

Cited by 7 publications

References 38 publications

Molecular identification and wing variations among malaria vectors in Akure North Local Government Area, Nigeria

Molecular identification and wing variations among malaria vectors in Akure North Local Government Area, Nigeria

Sibling species of the major malaria vector Anopheles gambiae display divergent preferences for aquatic breeding sites in southern Nigeria

The establishment of species-specific primers for the molecular identification of ten stored-product psocids based on ITS2 rDNA

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