The scarlet eye colour gene of the tephritid fruit fly: <i>Bactrocera tryoni</i> and the nature of two eye colour mutations

Zhao, J T; Bennett, Craig L.; Gj, Stewart; Frommer, Marianne; Raphael, Kathryn A.

doi:10.1046/j.1365-2583.2003.00410.x

Cited by 9 publications

(6 citation statements)

References 20 publications

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“…Outlier SNP 1 is located within the indel locus and situated approximately 56 kilobase pairs from the st gene (predicted gene g6239), coding the protein scarlet. The st gene is known to affect different aspects of coloration in several insect species [56–58] and was one of the prime candidate genes for cryptic body coloration identified in past Timema work [23,30].…”

Section: Resultsmentioning

confidence: 99%

Testing for fitness epistasis in a transplant experiment identifies a candidate adaptive locus inTimemastick insects

Villoutreix

Carvalho

Gompert

et al. 2022

Phil. Trans. R. Soc. B

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Identifying the genetic basis of adaptation is a central goal of evolutionary biology. However, identifying genes and mutations affecting fitness remains challenging because a large number of traits and variants can influence fitness. Selected phenotypes can also be difficult to know a priori , complicating top–down genetic approaches for trait mapping that involve crosses or genome-wide association studies. In such cases, experimental genetic approaches, where one maps fitness directly and attempts to infer the traits involved afterwards, can be valuable. Here, we re-analyse data from a transplant experiment involving Timema stick insects, where five physically clustered single-nucleotide polymorphisms associated with cryptic body coloration were shown to interact to affect survival. Our analysis covers a larger genomic region than past work and revealed a locus previously not identified as associated with survival. This locus resides near a gene, Punch ( Pu ) , involved in pteridine pigments production, implying that it could be associated with an unmeasured coloration trait. However, by combining previous and newly obtained phenotypic data, we show that this trait is not eye or body coloration. We discuss the implications of our results for the discovery of traits, genes and mutations associated with fitness in other systems, as well as for supergene evolution. This article is part of the theme issue ‘Genetic basis of adaptation and speciation: from loci to causative mutations’.

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Section: Resultsmentioning

confidence: 99%

Testing for fitness epistasis in a transplant experiment identifies a candidate adaptive locus inTimemastick insects

Villoutreix

Carvalho

Gompert

et al. 2022

Phil. Trans. R. Soc. B

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“…1995), Ceratitis capitata (Gomulski et al. 2001), Bactrocera dorsalis (Handler & McCombs 2000) and Bactrocera tryoni (Zhao et al. 2003).…”

Section: Discussionmentioning

confidence: 99%

Positional cloning of silkworm white egg 2 (w-2) locus shows functional conservation and diversification of ABC transporters for pigmentation in insects

Tatematsu¹,

Yamamoto²,

Uchino³

et al. 2011

Genes to Cells

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The white, scarlet and brown genes of Drosophila melanogaster encode three half-type ATP-binding cassette (ABC) transporters. In Drosophila, precursors of ommochromes and pteridines are transported by White ⁄ Scarlet and White ⁄ Brown heterodimers, respectively. The white egg 2 (w-2) mutant of the silkworm, Bombyx mori, has white eggs and eyes because of lack of ommochrome granules in the serosa and eyes. Here, we report that the silkworm w-2 locus encodes an ortholog of Drosophila scarlet. Our results indicate that Bombyx Scarlet forms a heterodimer with Bombyx White to transport ommochrome precursors, suggesting that formation of a White ⁄ Scarlet heterodimer and its involvement in the transport of ommochrome precursors are evolutionarily ancient and widely conserved traits in insects. Contrary to dipteran insects, white and scarlet were juxtaposed in a head-to-tail orientation in the silkworm genome, suggesting that the origin of white and scarlet was a tandem duplication of their ancestral transporter gene. In Bombyx, White is also essential for the transport of uric acid in larval epidermis. However, our results suggest that a Bombyx White ⁄ Scarlet heterodimer is not involved in this process. Our results emphasize the functional conservation and diversification of half-type ABC transporter families in insects, which may contribute to their extremely diverse color patterns.

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“…Embryos were collected from a B. tryoni lemon-eye (le) stock, homozygous for an eye-colour mutation with very light yellow eyes (Zhao et al 2003). Embryos were dechorionated for 1 min in 1% sodium hypochlorite and rinsed in 0.02% Triton-X100 and milliQ water.…”

Section: Microinjectionsmentioning

confidence: 99%

Germ-line transformation of the Queensland fruit fly, Bactrocera tryoni, using a piggyBac vector in the presence of endogenous piggyBac elements

et al. 2010

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We report the heritable germ-line transformation of the Queensland fruit fly, Bactrocera tryoni, using a piggyBac vector marked with either the fluorescent protein DsRed or EGFP. A transformation frequency of 5-10% was obtained. Inheritance of the transgenes has remained stable over more than 15 generations despite the presence of endogenous piggyBac sequences in the B. tryoni genome. The sequence of insertion sites shows the usual canonical pattern of piggyBac integraton into TTAA target sites. An investigation of endogenous piggyBac elements in the B. tryoni genome reveals the presence of sequences almost identical to those reported recently for the B. dorsalis complex of fruit flies and two noctuid moths, suggesting a common origin of piggyBac sequences in these species. The availability of transformation protocols for B. tryoni has the potential to deliver improvements in the performance of the Sterile Insect Technique for this pest species.

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The scarlet eye colour gene of the tephritid fruit fly: Bactrocera tryoni and the nature of two eye colour mutations

Cited by 9 publications

References 20 publications

Testing for fitness epistasis in a transplant experiment identifies a candidate adaptive locus inTimemastick insects

Testing for fitness epistasis in a transplant experiment identifies a candidate adaptive locus inTimemastick insects

Positional cloning of silkworm white egg 2 (w-2) locus shows functional conservation and diversification of ABC transporters for pigmentation in insects

Germ-line transformation of the Queensland fruit fly, Bactrocera tryoni, using a piggyBac vector in the presence of endogenous piggyBac elements

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