The SAM Domain of Human TEL2 Can Abrogate Transcriptional Output from TEL1 (ETV-6) and ETS1/ETS2

Vivekanand, Pavithra; Rebay, Ilaria

doi:10.1371/journal.pone.0037151

Cited by 11 publications

(10 citation statements)

References 30 publications

(68 reference statements)

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“…Tribolium ; Bucher and Klingler, 2005 ), echinoderms, and the chordate Ciona . Although no clear ortholog of Edl appears to be present in vertebrates, a SAM domain-only isoform of the human Yan-relative TEL2 as well as Drosophila Edl were shown to inhibit transcriptional stimulation by the mammalian Pnt orthologs ETS1/ETS2 in cell culture ( Gu et al, 2001 ; Vivekanand and Rebay, 2012 ). Hence, the restriction of ETS protein activities by protein-protein interactions offers an intriguing mechanism to fine-tune MAPK signaling output in developing tissues of both invertebrates and vertebrates.…”

Section: Discussionmentioning

confidence: 99%

Diversification of heart progenitor cells by EGF signaling and differential modulation of ETS protein activity

Schwarz

Hollfelder

Scharf

et al. 2018

eLife

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For coordinated circulation, vertebrate and invertebrate hearts require stereotyped arrangements of diverse cell populations. This study explores the process of cardiac cell diversification in the Drosophila heart, focusing on the two major cardioblast subpopulations: generic working myocardial cells and inflow valve-forming ostial cardioblasts. By screening a large collection of randomly induced mutants, we identified several genes involved in cardiac patterning. Further analysis revealed an unexpected, specific requirement of EGF signaling for the specification of generic cardioblasts and a subset of pericardial cells. We demonstrate that the Tbx20 ortholog Midline acts as a direct target of the EGFR effector Pointed to repress ostial fates. Furthermore, we identified Edl/Mae, an antagonist of the ETS factor Pointed, as a novel cardiac regulator crucial for ostial cardioblast specification. Combining these findings, we propose a regulatory model in which the balance between activation of Pointed and its inhibition by Edl controls cardioblast subtype-specific gene expression.

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Section: Discussionmentioning

confidence: 99%

Diversification of heart progenitor cells by EGF signaling and differential modulation of ETS protein activity

Schwarz

Hollfelder

Scharf

et al. 2018

eLife

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“…When we analyzed both TFs in the other two species, we only found differential expression of ets2 (Am < Pm; i.e., gradient in the same direction as tel2 ) in the straight-striped M. auratus . Interestingly, TEL2 can suppress ETS2 transcriptional activity and this mechanism appeared to be highly conserved in animal (between Drosophila and human) ( Vivekanand et al, 2012 ). Consequently, in A. christyi , the anterior-posterior variation in tel2 expression might cancel out the variation in ets2 expression, thus making the tel2 / ets2 expression patterns in the two oblique-striped species functionally equivalent.…”

Section: Discussionmentioning

confidence: 99%

Anterior-posterior gene expression differences in three Lake Malawi cichlid fishes with variation in body stripe orientation

Ahi

Sefc

2017

PeerJ

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Morphological differentiation among closely related species provides opportunities to study mechanisms shaping natural phenotypic variation. Here, we address variation in the orientation of melanin-colored body stripes in three cichlid species of the tribe Haplochromini. Melanochromis auratus displays a common pattern of dark, straight horizontal body stripes, whereas in Aristochromis christyi and Buccochromis rhoadesii, oblique stripes extend from the anterior dorsal to the posterior mid-lateral trunk. We first validated a stably reference gene, and then, investigated the chromatophore distribution in the skin by assessing the expression levels of the iridophore and melanophore marker genes, ltk and slc24a5, respectively, as well as pmel, a melanophore pigmentation marker gene. We found anterior-posterior differences in the expression levels of the three genes in the oblique-striped species. The higher anterior expression of ltk, indicates increased iridophore density in the anterior region, i.e., uneven horizontal distribution of iridophores, which coincides with the anterior dorsalization of melanophore stripe in these species. The obliqueness of the horizontal body stripes might be a result of distinct migratory or patterning abilities of melanophores in anterior and posterior stripe regions which could be reflected by variation in the expression of genes involved in melanophore patterning. To address this, we investigated anterior-posterior expression levels of a primary set of candidate target genes with known functions in melanophore migration and stripe patterning in the adult zebrafish, and their related gene regulatory network. Among these genes, those with differences in anterior-posterior expression showed only species-specific differential expression, e.g., sdf1a, col14a1a, ifitm5, and agpat3, with the exception of fbxw4/hagoromo (differentially expressed in an oblique-and the straight-striped species). In summary, distinct anterior-posterior gradients in iridophore density found to be more similar characteristic between the two oblique-striped species. Furthermore, the species-specific differential expression of genes involved in stripe patterning might also implicate distinct molecular processes underlying the obliqueness of body stripe in two closely related cichlid species.

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“…Here, we found that Hmbox1 positively regulated CD163, while CD163 might be regulated by Ets-2 [ 34 ]. Meanwhile, plenty of reports showed that Ets-1 and Ets-2 are functionally redundant in lots of cases and required for endothelial cell survival [ 35 – 38 ]. It is a possible pathway that CD163 is positively regulated by Hmbox1/Ets-1 signaling, and, in our further investigation, we will verify this pathway of Hmbox1/Ets-1/CD163 signaling.…”

Section: Discussionmentioning

confidence: 99%

The New Role of CD163 in the Differentiation of Bone Marrow Stromal Cells into Vascular Endothelial‐Like Cells

et al. 2016

Stem Cells International

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Bone marrow stromal cells (BMSCs) can differentiate into vascular endothelial cells (VECs). It is regarded as an important solution to cure many diseases, such as ischemic diseases and diabetes. However, the mechanisms underlying BMSC differentiation into VECs are not well understood. Recent reports showed that CD163 expression was associated with angiogenesis. In this study, overexpression of CD163 in BMSCs elevated the protein level of the endothelial-associated markers CD31, Flk-1, eNOS, and VE-cadherin, significantly increased the proportion of Alexa Fluor 488-acetylated-LDL-positive VECs, and promoted angiogenesis on Matrigel. Furthermore, we demonstrated that CD163 acted downstream homeobox containing 1 (Hmbox1) and upstream fibroblast growth factor 2 (FGF-2). These data suggested that CD163 was involved in Hmbox1/CD163/FGF-2 signal pathway in BMSC differentiation into vascular endothelial-like cells. We found a new signal pathway and a novel target for further investigating the gene control of BMSC differentiation into a VEC lineage.

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The SAM Domain of Human TEL2 Can Abrogate Transcriptional Output from TEL1 (ETV-6) and ETS1/ETS2

Cited by 11 publications

References 30 publications

Diversification of heart progenitor cells by EGF signaling and differential modulation of ETS protein activity

Diversification of heart progenitor cells by EGF signaling and differential modulation of ETS protein activity

Anterior-posterior gene expression differences in three Lake Malawi cichlid fishes with variation in body stripe orientation

The New Role of CD163 in the Differentiation of Bone Marrow Stromal Cells into Vascular Endothelial‐Like Cells

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