The New Role of CD163 in the Differentiation of Bone Marrow Stromal Cells into Vascular Endothelial‐Like Cells

Lu, Wenjing; Su, Le; Yu, Zhezheng; Zhang, Shangli; Jiang, Miao

doi:10.1155/2016/2539781

Cited by 13 publications

(7 citation statements)

References 40 publications

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“…51 Disappointingly, these agents have not been successful in trials, which may be because increased levels of TAMs may still be present after anti-angiogenesis therapies; the number of CD163+ TAMs significantly correlated with an overall decreased survival among patients with recurrent GBM. 14 Recently, Wei Lu 52 demonstrated that overexpressed CD163 in bone marrow stromal cells (BMSCs) elevated protein levels of endothelial-associated markers such as CD31, Flk-1, eNOS, and VE-cadherin, significantly promoting angiogenesis. They also found that CD163 was involved in Hmbox1/CD163/FGF-2 signal pathway in BMSC differentiation into vascular endothelial-like cells, which suggested that CD163 was a key regulator in angiogenesis and provided a novel target for further investigating the gene control of BMSC differentiation into vascular endothelial-like cells.…”

Section: Discussionmentioning

confidence: 99%

Molecular and clinical characterization of CD163 expression via large-scale analysis in glioma

Zhang

Maimela

et al. 2019

OncoImmunology

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The expression and function of CD163 in glioma are not fully understood. In this report, we collected totally 1323 glioma samples from the Chinese Glioma Genome Atlas (CGGA) dataset, including 325 RNA-seq data and 301 mRNA microarray data, and 697 glioma samples from The Cancer Genome Atlas (TCGA) dataset to characterize the molecular and clinical features of CD163 in glioma by conducting a large-scale study. We found that CD163 expression was positively associated with the grade of malignancy of glioma. CD163 expression was up-regulated in IDH wild-type glioma and mesenchymal subtype. Gene ontology analysis suggested that CD163-related genes were more involved in immune response and angiogenesis in glioma. Moreover, CD163 showed a positive relationship with stromal and immune cell populations. Kaplan-Meier curves analysis revealed that higher CD163 expression indicated significantly poor survival in glioma and glioblastoma multiforme (GBM). Pearson correlation analysis revealed that CD163 was robustly associated with the immune checkpoints and other macrophage markers. These results demonstrated that CD163 predicts poor prognosis in glioma patients. Additionally, combination of CD163 and immune checkpoints may impair angiogenesis and reverse dysfunctional phenotypes of T cells, which suggest that CD163 may be a promising biomarker and target for immunotherapeutic strategies.

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Section: Discussionmentioning

confidence: 99%

Molecular and clinical characterization of CD163 expression via large-scale analysis in glioma

Zhang

Maimela

et al. 2019

OncoImmunology

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“…9 CD163 is also a marker of alternatively activated anti-inflammatory macrophages/microglia that are abundant during the resolution phase of the inflammatory process, and has been postulated to participate in angiogenic repair mechanisms. [10][11][12] It is of considerable interest that no studies have evaluated the role of CD163 after ICH, given its aforementioned functions and reports using human autopsy specimens and a porcine model demonstrating that CD163-positive macrophages/microglia accumulate in the brain following ICH. [13][14][15] Using CD163-deficient mice, we reveal that CD163 has distinct temporal influences on ICH outcomes, with early deleterious properties and delayed beneficial effects.…”

Section: Introductionmentioning

confidence: 99%

The absence of the CD163 receptor has distinct temporal influences on intracerebral hemorrhage outcomes

Leclerc

Lampert

Amador

et al. 2017

J Cereb Blood Flow Metab

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Hemoglobin (Hb) toxicity precipitates secondary brain damage following intracerebral hemorrhage (ICH). CD163 is an anti-inflammatory Hb scavenger receptor and CD163-positive macrophages/microglia locally accumulate post-bleed, yet no studies have investigated the role of CD163 after ICH. ICH was induced in wildtype and CD163 mice and various anatomical and functional outcomes were assessed. At 3 d, CD163 mice have 43.4 ± 5.0% (p = 0.0002) and 34.8 ± 3.4% (p = 0.0003) less hematoma volume and tissue injury, respectively. Whereas, at 10 d, CD163 mice have 49.2 ± 15.0% larger lesions (p = 0.0385). An inflection point was identified, where CD163 mice perform better on neurobehavioral testing and have less mortality before 4 d, but increased mortality and worse function after 4 d (p = 0.0389). At 3 d, CD163 mice have less Hb, iron, and blood-brain barrier dysfunction, increased astrogliosis and neovascularization, and no change in heme oxygenase 1 (HO1) expression. At 10 d, CD163 mice have increased iron and VEGF immunoreactivity, but no significant change in HO1 or astrogliosis. These novel findings reveal that CD163 deficiency has distinct temporal influences following ICH, with early beneficial properties but delayed injurious effects. While it is unclear why CD163 deficiency is initially beneficial, the late injurious effects are consistent with the key anti-inflammatory role of CD163 in the recovery phase of tissue damage.

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“…MSCs have been reported to be able to differentiate into VSMC-like or EC-like cells with the stimulation of TGFβ1 or VEGF, respectively. − Moreover, it is well recognized that angiogenesis is regulated by mechanobiological factors, such as substrate stiffness and geometrical confinements . Increasing evidence has demonstrated that tissue stiffness and mechanical stimuli are the key cellular microenvironment.…”

Section: Results and Discussionmentioning

confidence: 99%

Fabrication and Characterization of Pectin Hydrogel Nanofiber Scaffolds for Differentiation of Mesenchymal Stem Cells into Vascular Cells

Xue

Zhang

et al. 2019

ACS Biomater. Sci. Eng.

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Despite significant progress over the past few decades, creating a tissue-engineered vascular graft with replicated functions of native blood vessels remains a challenge due to the mismatch in mechanical properties, low biological function, and rapid occlusion caused by restenosis of small diameter vessel grafts (<6 mm diameter). A scaffold with similar mechanical properties and biocompatibility to the host tissue is ideally needed for the attachment and proliferation of cells to support the building of engineered tissue. In this study, pectin hydrogel nanofiber scaffolds with two different oxidation degrees (25 and 50%) were prepared by a multistep methodology including periodate oxidation, electrospinning, and adipic acid dihydrazide crosslinking. Scanning electron microscopy (SEM) images showed that the obtained pectin nanofiber mats have a nano-sized fibrous structure with 300–400 nm fiber diameter. Physicochemical property testing using Fourier transform infrared (FTIR) spectra, atomic force microscopy (AFM) nanoindentations, and contact angle measurements demonstrated that the stiffness and hydrophobicity of the fiber mat could be manipulated by adjusting the oxidation and crosslinking levels of the pectin hydrogels. Live/Dead staining showed high viability of the mesenchymal stem cells (MSCs) cultured on the pectin hydrogel fiber scaffold for 14 days. In addition, the potential application of pectin hydrogel nanofiber scaffolds of different stiffness in stem cell differentiation into vascular cells was assessed by gene expression analysis. Real-time polymerase chain reaction (RT-PCR) results showed that the stiffer scaffold facilitated the differentiation of MSCs into vascular smooth muscle cells, while the softer fiber mat promoted MSC differentiation into endothelial cells. Altogether, our results indicate that the pectin hydrogel nanofibers have the capability of providing mechanical cues that induce MSC differentiation into vascular cells and can be potentially applied in stem cell-based tissue engineering.

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The New Role of CD163 in the Differentiation of Bone Marrow Stromal Cells into Vascular Endothelial‐Like Cells

Cited by 13 publications

References 40 publications

Molecular and clinical characterization of CD163 expression via large-scale analysis in glioma

Molecular and clinical characterization of CD163 expression via large-scale analysis in glioma

The absence of the CD163 receptor has distinct temporal influences on intracerebral hemorrhage outcomes

Fabrication and Characterization of Pectin Hydrogel Nanofiber Scaffolds for Differentiation of Mesenchymal Stem Cells into Vascular Cells

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