The safe use of Doliocarpus dentatus in the gestational period: Absence of changes in maternal reproductive performance, embryo-fetal development and DNA integrity

Ishikawa, Raissa Borges; Vani, Juliana Miron; Neves, Silvia Cordeiro das; Rabacow, Ana Paula Maluf; Kassuya, Cândida Aparecida Leite; Croda, Júlio; Cardoso, Cláudia Andréa Lima; Monreal, Antônio Carlos Duenhas; Antoniolli, Andréia Conceição Milan Brochado; Laura, Andréa Luiza Cunha –; Oliveira, Rodrigo Juliano

doi:10.1016/j.jep.2018.01.034

Cited by 12 publications

(12 citation statements)

References 14 publications

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“…The EEPg did not alter the reproductive performance since no alteration was observed in the number of pregnant females, of implantations, of live and dead fetuses and of resorptions nor in the rates of post-implantation losses, resorption rate, fetal viability and sex ratio. These parameters are those considered by the literature in the area to predict the possible deleterious effect of extracts and/or compounds on the performance of females [ 17 , 18 , 19 , 20 , 21 , 22 ]. However, EEPg altered embryofetal development.…”

Section: Discussionmentioning

confidence: 99%

“…However, in general, fetuses reduce their growth and/or stop gaining weight when there is a reduction in placental efficiency. However, the literature indicates different correlations between fetal weight, adequacy of weight for gestational age, placental weight, placental index and placental efficiency [ 17 , 18 , 19 , 21 , 22 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…This type of malformation can regress at birth and can be detected in the offspring of mothers whose pregnancies were terminated early. Thus, prematurely collected fetuses may present these variants of normality [ 17 , 18 , 38 ]. We collected fetuses on the 18th gestational day, which, despite being early given that the pregnancy can last up to the 21st gestational day, is the day recommended by the specialized literature [ 17 , 18 , 38 ]).…”

Section: Discussionmentioning

confidence: 99%

“…The animals were mated overnight at a ratio of 2 females: 1 male. Pregnancy detection was performed by observing the vaginal plug, which is considered day zero of pregnancy [ 7 , 17 , 18 , 24 , 25 , 38 ]. The pregnant animals were randomly distributed among the experimental groups ( n = 10): Control—the animals received the EEPg vehicle (Tween 80–1%) in the proportion of 0.1 mL/10 g of body weight (b.w.…”

Section: Methodsmentioning

confidence: 99%

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The Ethanolic Extract of Piper glabratum Kunth Is Teratogenic and Interferes with the Ossification Process of Swiss Mice Fetuses

et al. 2023

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Piper glabratum Kunth is a plant traditionally used to treat pain and inflammation in the Mato Grosso do Sul, Brazil. Even pregnant women consume this plant. Toxicology studies of the ethanolic extract from the leaves of P. glabratum (EEPg) could establish the safety of popular use of P. glabratrum. Thus, the effects of the ethanolic extract of leaves of P. glabratum (EEPg) on the reproductive performance and embryofetal development of Swiss mice were evaluated. Pregnant female mice were treated with 100, 1000 and 2000 mg/kg throughout the gestational period by gavage (p.o). The control group received the EEPg vehicle (Tween 80–1%) in the proportion of 0.1 mL/10 g (p.o.). The results demonstrated that EEPg has low maternal toxic potential and does not alter the reproductive performance of females. However, it altered embryofetal development and caused fetal weight reduction (increasing the frequency of small-for-gestational-age fetuses) at the two highest doses. In addition, it interfered with placental weight, placental index and placental efficiency. The frequency of visceral malformations increased by 2.8 times for the lowest dose of EEPg, and skeletal malformations increased by 2.48, 1.89 and 2.11 times for doses of 100, 1000 and 2000 mg/kg of EEPg, respectively. It is noteworthy that 100% of the offspring treated with EEPg showed changes in the ossification process. Thus, it is considered that the EEPg has low maternal toxic potential; it does not alter the reproductive performance of females. However, it is teratogenic and interferes, mainly, in the ossification process, and therefore its use is contraindicated in the gestational period.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Ethanolic Extract of Piper glabratum Kunth Is Teratogenic and Interferes with the Ossification Process of Swiss Mice Fetuses

et al. 2023

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“…Some studies in the field [ 27 , 50 ] describe variants of normality as changes that may regress as gestation progresses or at birth since the fetuses would have completed their development.…”

Section: Discussionmentioning

confidence: 99%

The ethanolic extract of Salvia lachnostachys Benth is not maternotoxic, does not alter reproductive performance, but has teratogenic potential

Ortiz

Neves

Kassuya

et al. 2023

BMC Complement Med Ther

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Salvia lachnostachys Benth is native to Brazil and has anti-inflammatory, anti-arthritic, cytotoxic, antitumor, and antihyperalgesic activities. The population, including pregnant women, consume this plant to treat pain, inflammation, flu, spasms, insomnia, and depression, mainly. There are no safety reports on the use of this plant during pregnancy. The present study aimed to evaluate the effects of S. lachnostachys ethanolic extract (EESl) on reproductive performance, embryofetal development, and DNA integrity of pregnant female mice. Pregnant females were randomly divided into three experimental groups (n = 10): The Control group was treated with a vehicle, and treatment groups were administered with EESl at 100 and 1000 mg/kg, respectively. Treatment occurred by gavage throughout the gestational period until day 18. Afterward, reproductive performance, embryofetal development, and DNA integrity parameters were evaluated. The results indicated that EESl did not alter any reproductive performance parameters. However, it changed embryofetal outcome through reduced placental weight (EESl 100 mg/kg), decreased fetal weight (EESl 100 and 1000 mg/kg), and increased frequency of small for gestational age fetuses (EESl 1000 mg/kg). In addition, EES1 increased the frequency of external, visceral, and skeletal malformations. Because of the above, it is considered that EESl is not maternotoxic, does not alter reproductive performance, but does alter embryofetal development. Its use in the gestational period is not indicated due to its teratogenic potential.

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Aqueous extract from leaves of Doliocarpus dentatus (Aubl.) Standl. relieves pain without genotoxicity activity

Branquinho

Verdan

Santos

et al. 2021

Journal of Ethnopharmacology

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The safe use of Doliocarpus dentatus in the gestational period: Absence of changes in maternal reproductive performance, embryo-fetal development and DNA integrity

Abstract: the EEDd do not alter the reproductive parameters, embryofetal development and DNA integrity, ensuring its safe use during pregnancy.

Cited by 12 publications

References 14 publications

The Ethanolic Extract of Piper glabratum Kunth Is Teratogenic and Interferes with the Ossification Process of Swiss Mice Fetuses

The Ethanolic Extract of Piper glabratum Kunth Is Teratogenic and Interferes with the Ossification Process of Swiss Mice Fetuses

The ethanolic extract of Salvia lachnostachys Benth is not maternotoxic, does not alter reproductive performance, but has teratogenic potential

Aqueous extract from leaves of Doliocarpus dentatus (Aubl.) Standl. relieves pain without genotoxicity activity

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