Aqueous extract from leaves of Doliocarpus dentatus (Aubl.) Standl. relieves pain without genotoxicity activity

Branquinho, Lidiane Schultz; Verdan, Maria Helena; Santos, Elisângela dos; Neves, Silvia Cordeiro das; Oliveira, Rodrigo Juliano; Cardoso, Cláudia Andréa Lima; Kassuya, Cândida Aparecida Leite

doi:10.1016/j.jep.2020.113440

Cited by 6 publications

(8 citation statements)

References 59 publications

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“…Several candidates of D. dentatus red and white putative metabolites were then shortlisted. Those that showed identical names from KnapSack [33] (Shinbo, Nakamura et al 2006), KEGG [34] (Kanehisa, Sato et al 2019), the Plant Metabolic Network using the PlantCyc database (https://plantcyc.org, accessed on 10 June 2023) [35], MetaboQuest [36], SciFinder [37] and PubChem [38], Global Nature Production Social Molecular Networking (GNPS), SIRIUS, and MS Dial were used to query fragments for putative identifications [3,15]. Then, peak confirmation was performed with Xcalibur 4.1 having a mass error of ±10 ppm to determine whether these metabolites were present in the methanol extracts of D. dentatus red and white ecotypes.…”

Section: Discussionmentioning

confidence: 99%

“…D. dentatus leaves' ethanolic extract was evaluated for widespread use against pain in animal models and its toxicogenicity [15]. The results indicated that an ethnopharmacological dose of the aqueous extract from the leaves of D. dentatus decreased the pain response, indicating analgesic and anti-inflammatory actions.…”

Section: Introductionmentioning

confidence: 99%

“…The results indicated that an ethnopharmacological dose of the aqueous extract from the leaves of D. dentatus decreased the pain response, indicating analgesic and anti-inflammatory actions. Furthermore, toxicogenic assays determined that the aqueous extract of D. dentatus did not have genotoxic potential and did not modify splenic phagocytosis [3,15]. Another study suggested that the extract from the leaves of Capadulla had antibacterial and anti-inflammatory properties, demonstrating that the leaves had phytochemical compounds that could treat various illnesses [13,16].…”

Section: Introductionmentioning

confidence: 99%

“…In vitro studies of the biological activity of D. dentatus have shown that it is an antimicrobial against strains of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus; anti-inflammatory; and cytotoxic in leukemic cells of the K562 lineage [13]. It is effective against amastigotes of Leishmania amazonenses [17,18] and does not cause genomic or chromosomal damage, implying that it is safe for use [14][15][16]19].…”

Section: Introductionmentioning

confidence: 99%

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Unlocking Potentially Therapeutic Phytochemicals in Capadulla (Doliocarpus dentatus) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics

Smith,

Lewis,

Narine

et al. 2023

Metabolites

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Doliocarpus dentatus is thought to have a wide variety of therapeutic phytochemicals that allegedly improve libido and cure impotence. Although a few biomarkers have been identified with potential antinociceptive and cytotoxic properties, an untargeted mass spectrometry-based metabolomics approach has never been undertaken to identify therapeutic biofingerprints for conditions, such as erectile dysfunction, in men. This study executes a preliminary phytochemical screening of the woody vine of two ecotypes of D. dentatus with renowned differences in therapeutic potential for erectile dysfunction. Liquid chromatography–mass spectrometry-based metabolomics was used to screen for flavonoids, terpenoids, and other chemical classes found to contrast between red and white ecotypes. Among the metabolite chemodiversity found in the ecotype screens, using a combination of GNPS, MS-DIAL, and SIRIUS, approximately 847 compounds were annotated at levels 2 to 4, with the majority of compounds falling under lipid and lipid-like molecules, benzenoids and phenylpropanoids, and polyketides, indicative of the contributions of the flavonoid, shikimic acid, and terpenoid biosynthesis pathways. Despite the extensive annotation, we report on 138 tentative compound identifications of potentially therapeutic compounds, with 55 selected compounds at a level-2 annotation, and 22 statistically significant therapeutic biomarkers, the majority of which were polyphenols. Epicatechin methyl gallate, catechin gallate, and proanthocyanidin A2 had the greatest significant differences and were also relatively abundant among the red and white ecotypes. These putatively identified compounds reportedly act as antioxidants, neutralizing damaging free radicals, and lowering cell oxidative stress, thus aiding in potentially preventing cellular damage and promoting overall well-being, especially for treating erectile dysfunction (ED).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Unlocking Potentially Therapeutic Phytochemicals in Capadulla (Doliocarpus dentatus) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics

Smith,

Lewis,

Narine

et al. 2023

Metabolites

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show abstract

“…e clinical analgesic effect of chemical drugs has been fully recognized, but for patients with chronic pain, long-term use will produce dependence, peptic ulcer, and other adverse reactions. Accordingly, with the development of traditional Chinese medicine industry, the research of natural plant extracts for pain treatment is gradually deepening [36,37]. Botanical medicine is an important method for the treatment of cancer pain.…”

Section: Discussionmentioning

confidence: 99%

The Pharmacological Mechanism of the Effect of Plant Extract Compound Drugs on Cancer Pain Based on Network Pharmacology

Shen¹,

Wang²,

Yan³

et al. 2022

Journal of Healthcare Engineering

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Objective. We systematically analyzed the mechanism of plant-derived drugs alleviating cancer pain in our hospital through network pharmacology, so as to provide the possibility of further application of traditional Chinese medicine in the treatment of cancer pain. Methods. We used TCMSP, ETCM, and TCMID databases to mine the active ingredients of plant-derived drugs. We combined OMIM, GeneCards, and DrugBank databases to mine and match the common targets of plant-derived drugs for cancer pain. We used the STRING platform and Cytoscape software to analyze and screen out the core targets. We used GO and KEGG methods to analyze the biological processes, molecular functions, cellular composition, and signaling pathways involved in the reduction of cancer pain by plant-derived drugs. Results. We found 153 active ingredients from botanical drugs by TCMSP (Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, TCMSP), ETCM (The Encyclopedia of Traditional Chinese Medicine), and TCMID (Traditional Chinese Medicine Integrated Database) databases, covering 341 protein targets in human body. Combined with OMIM (Online Mendelian Inheritance in Man), GeneCards, and DrugBank databases, we excavated and matched 141 targets of plant-derived drugs and cancerous pain diseases. Through the analysis of the STRING platform and Cytoscape software, 19 core targets including TNF, MAPK1, JUN, and IL-6 were screened out. Go and KEGG enrichment showed that plant-derived drugs alleviated cancer pain processes involving 193 biological processes, 47 molecular functions, 22 cell components, and 118 signaling pathways. By screening genes involved in KEGG signaling pathway, it was found that plant-derived drugs were mainly associated with PI3K-Akt signaling pathway, tumor necrosis factor signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway, and HIF-1 signaling pathway in alleviating cancer pain. Conclusion. These results indicate that botanical drugs can positively affect the expression of inflammatory factors and apoptotic factors in the process of treatment and relief of cancer pain, which is expected to have a potential therapeutic effect on the relief of cancer pain.

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Chemical composition and antinociceptive and anti-inflammatory activity of the essential oil of Hyptis crenata Pohl ex Benth. from the Brazilian Amazon

Lima

Guimarães

Castro

et al. 2023

Journal of Ethnopharmacology

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Aqueous extract from leaves of Doliocarpus dentatus (Aubl.) Standl. relieves pain without genotoxicity activity

Cited by 6 publications

References 59 publications

Unlocking Potentially Therapeutic Phytochemicals in Capadulla (Doliocarpus dentatus) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics

Unlocking Potentially Therapeutic Phytochemicals in Capadulla (Doliocarpus dentatus) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics

The Pharmacological Mechanism of the Effect of Plant Extract Compound Drugs on Cancer Pain Based on Network Pharmacology

Chemical composition and antinociceptive and anti-inflammatory activity of the essential oil of Hyptis crenata Pohl ex Benth. from the Brazilian Amazon

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