The Routine and Specialised Staining for the Histologic Evaluation of Autogenous Mandibular Bone Grafts An experimental study

Abstract

Oral rehabilitation by dental implants is a routine treatment in the common dental practice, and volume reconstruction in cases of advanced alveolar ridge atrophy using bone autografts has become a frequently used therapeutic procedure. The study presents a histological evaluation of autogenous mandibular bone grafts integration in surgically created maxillary bone defects. Seven domestic adult dogs, Canis Familiaris were used in the study. Work methodology was established through maxillary and mandibular morphometry, the donor region being the posterior mandibular body, and the recipient region being the lateral body of the maxilla. In the experimental study, we simulated two bilateral maxillary bone defects, which were augmented with mandibular corticocancellous bone grafts. Biological samples containing the target areas were collected 90-100 days after grafting and the subsequent preparation method of the samples for histological analysis was the standard one.The histological results showed the successful integration and the beneficial effect of corticocancellous autogenous mandibular bone grafts applied in maxillary sites.

Effect of Tooth Brushing on Wear of Microhybrid Composites In vitro experiment

“…In Romanian Dental Medicine there are also other recent in vivo and in vitro experimental study, performed especially in the fields of implantology and endodontics [32][33][34][35].…”

Experimental model for optimizing dental implant therapy in the posterior maxilla

“…On such experimental models, histopathological and immunohistochemical studies on autogenous bone grafts integration and bone, epithelium and connective tissue integration to dental implants can be further performed (V. NIMIGEAN & al [28], V. NIMIGEAN & al [29], V. NIMIGEAN & al [30]). Other experimental models were aimed at investigating the histological and immunohistochemical changes of pulp tissue exposed to different biomaterials (M.J. TUCULINA & al [31]).…”

Analysis of the intricate effects of polyunsaturated fatty acids and polyphenols on inflammatory pathways in health and disease

“…Under the effect of inflammatory stimuli (for instance cytokines -IL-8, IL-1β, IL-6, or TNF-α, UV exposure, etc), the heterodimer dissociates, IκBα is degraded, and the two components, p50 and p65, translocate into the nucleus binding the promoter regions from different genes that are involved in initiating several cellular pathways linked to chronic diseases, tumorigenesis, angiogenesis and metastasis. Among the genes regulated by NF-κB are the ones for the expression of TNF-α, COX-2, MMP-9, nitric oxide synthasethe inducible form (iNOS), cytokines (IL-1, IL-6 and IL-8), 5-lipooxigenase (5-LOX), vascular endothelial growth factor (VEGF); all of these genes, when up-regulated fireup the vicious circle constituted from oxidative stress and inflammation (Aggarwal, 2009;Kawabata et al, 2012;Kunnumakkara et al, 2018;Nimigean et al, 2018Nimigean et al, , 2019Poll et al, 2018aPoll et al, , 2018bReuter et al, 2010). Interestingly, the NF-κB cascade is also activated by some factors that affect the circadian rhythm, such as aging or sleep deprivation.…”