To investigate the case about room air pollution by microorganism from some region of Gannan Medical University and the effect of disinfectant made from traditional chinese medicine on room air. By flat natural precipitation method, the six sampling zones which were classroom, microorganism teaching laboratory, teacher’s office, the corridor of out-patient department, in-patient department hall and department of dermatology were selected to observe the case about room air pollution before disinfectant and the disinfection effect after disinfected with disinfection maken from traditional chinese medicine which named KZY-2 composed by Anemarrhena asphodeloides and Rhizoma coptidis, effect of disinfectant of KZY-2 on teaching laboratory was compared with ultraviolet ray by counting colonies of bacteria and fungi respectively. Total number of colony of the sampling zones from most to least was the corridor of out-patient department, department of dermatology, in-patient department hall, classroom, teaching laboratory, teacher’s office, number of fungal colony was department of dermatology, the corridor of out-patient department, in-patient department hall, teaching laboratory, classroom, teacher’s office, the average number of colony from the sampling sites of hospital (6764.3 cfu/m3) was significantly higher than campus (2190.7 cfu/m3),P<0.01. Total number of colony of the sampling sites was significantly decreased after disinfected with KZY-2 and ultraviolet ray. The sterilizing rate of KZY-2 was average 82.9% and up to 90.1%, the sterilizing rate of ultraviolet ray was average 63.5%,the disinfection effect between KZY-2 and ultraviolet ray was differential (P<0.05). These results indicate that KZY-2 and ultraviolet ray all have degermation on air and the disinfection effect of KZY-2 is superior to ultraviolet ray.