The Roles of Insulin-Like Growth Factor Binding Protein Family in Development and Diseases

Song, Fei; Zhou, Xiaoxia; Hu, Yu Feng; Li, Gang; Wang, Yan

doi:10.1007/s12325-020-01581-x

Cited by 17 publications

(21 citation statements)

References 96 publications

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“…IGF is instrumental in growth regulation, development, and immune response ( 1 , 4 , 39 ). Meanwhile, IGFBPs have crucial roles due to their high binding affinity to IGFs ( 3 , 40 ).…”

Section: Discussionmentioning

confidence: 99%

“…The insulin-like growth factor (IGF) system is mainly composed of IGF-I/II, IGF receptors, and IGF-binding proteins (IGFBPs) ( 1 ). IGFBPs act as IGF carriers and regulate their biological distribution ( 2 ).…”

Section: Introductionmentioning

confidence: 99%

“…IGFBPs act as IGF carriers and regulate their biological distribution ( 2 ). The IGF signaling pathway has been proven to be crucial for the onset and progression of numerous diseases as well as the control of cellular activities ( 1 , 3 ). Given the extensive evidence regarding the significance of IGF, the IGFBP family, which was identified and designated IGFBP1 to IGFBP6, has attracted much attention in recent years ( 4 , 5 ).…”

Section: Introductionmentioning

confidence: 99%

“…In mammals, IGFBP5 plays a variety of functions in cellular activities and has been reported to be associated with many diseases ( 1 , 4 ). Studies found that the expression of IGFBP5 may stimulate retinal pigment epithelium (RPE) cell fibrosis, leading to the progression of proliferative vitreoretinopathy ( 11 , 12 ).…”

Section: Introductionmentioning

confidence: 99%

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Insulin-like growth factor binding protein 5b of Trachinotus ovatus and its heparin-binding motif play a critical role in host antibacterial immune responses via NF-κB pathway

Zhou²,

Du³

et al. 2023

Front. Immunol.

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IntroductionInsulin-like growth factor binding protein 5 (IGFBP5) exerts an essential biological role in many processes, including apoptosis, cellular differentiation, growth, and immune responses. However, compared to mammalians, our knowledge of IGFBP5 in teleosts remains limited.MethodsIn this study, TroIGFBP5b, an IGFBP5 homologue from golden pompano (Trachinotus ovatus) was identified. Quantitative real-time PCR (qRT-PCR) was used to check its mRNA expression level in healthy condition and after stimulation. In vivo overexpression and RNAi knockdown method were performed to evaluate the antibacterial profile. We constructed a mutant in which HBM was deleted to better understand the mechanism of its role in antibacterial immunity. Subcellular localization and nuclear translocation were verified by immunoblotting. Further, proliferation of head kidney lymphocytes (HKLs) and phagocytic activity of head kidney macrophages (HKMs) were detected through CCK-8 assay and flow cytometry. Immunofluorescence microscopy assay (IFA) and dual luciferase reporter (DLR) assay were used to evaluate the activity in nuclear factor-κB (NF-κβ) pathway.ResultsThe TroIGFBP5b mRNA expression level was upregulated after bacterial stimulation. In vivo, TroIGFBP5b overexpression significantly improved the antibacterial immunity of fish. In contrast, TroIGFBP5b knockdown significantly decreased this ability. Subcellular localization results showed that TroIGFBP5b and TroIGFBP5b-δHBM were both present in the cytoplasm of GPS cells. After stimulation, TroIGFBP5b-δHBM lost the ability to transfer from the cytoplasm to the nucleus. In addition, rTroIGFBP5b promoted the proliferation of HKLs and phagocytosis of HKMs, whereas rTroIGFBP5b-δHBM, suppressed these facilitation effects. Moreover, the in vivo antibacterial ability of TroIGFBP5b was suppressed and the effects of promoting expression of proinflammatory cytokines in immune tissues were nearly lost after HBM deletion. Furthermore, TroIGFBP5b induced NF-κβ promoter activity and promoted nuclear translocation of p65, while these effects were inhibited when the HBM was deleted.DiscussionTaken together, our results suggest that TroIGFBP5b plays an important role in golden pompano antibacterial immunity and activation of the NF-κβ signalling pathway, providing the first evidence that the HBM of TroIGFBP5b plays a critical role in these processes in teleosts.

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“…IGF is instrumental in growth regulation, development, and immune response ( 1 , 4 , 39 ). Meanwhile, IGFBPs have crucial roles due to their high binding affinity to IGFs ( 3 , 40 ).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Insulin-like growth factor binding protein 5b of Trachinotus ovatus and its heparin-binding motif play a critical role in host antibacterial immune responses via NF-κB pathway

Zhou²,

Du³

et al. 2023

Front. Immunol.

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show abstract

“…The IGF and IGF-binding proteins (IGFBPs) are primarily synthesized in the liver and constitute a complex system that plays a critical role in cellular proliferation and differentiation [ 14 , 15 ]. Growth hormone (GH), secreted from the somatotrophs in the anterior pituitary, drives the synthesis of IGF-1 in the liver.…”

Section: The Liver As An Endocrine Organmentioning

confidence: 99%

Hepatocrinology

2021

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Hepatocrinology is defined as a bidirectional, complex relationship between hepatic physiology and endocrine function, hepatic disease and endocrine dysfunction, hepatotropic drugs and endocrine function, and endocrine drugs and hepatic health. The scope of hepatocrinology includes conditions of varied etiology (metabolic, infectious, autoimmune, and invasive) that we term as hepato-endocrine syndromes. This perspective shares the definition, concept, and scope of hepatocrinology and shares insight related to this aspect of medicine. It is hoped that this communication will encourage further attention and research in this critical field.

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Effect of IGFBP6 Knockdown on Proteins Regulating Exosome Synthesis and Secretion in MDA-MB-231 Cell Line

et al. 2023

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The Roles of Insulin-Like Growth Factor Binding Protein Family in Development and Diseases

Cited by 17 publications

References 96 publications

Insulin-like growth factor binding protein 5b of Trachinotus ovatus and its heparin-binding motif play a critical role in host antibacterial immune responses via NF-κB pathway

Insulin-like growth factor binding protein 5b of Trachinotus ovatus and its heparin-binding motif play a critical role in host antibacterial immune responses via NF-κB pathway

Hepatocrinology

Effect of IGFBP6 Knockdown on Proteins Regulating Exosome Synthesis and Secretion in MDA-MB-231 Cell Line

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