The roles of free oxygen radicals, nitric oxide, and endothelin in caustic injury of rat esophagus

Özel, Kerem; Dağlı, Tolga E.; Yüksel, Meral; Kıyan, Gürsu; Kotiloǧlu, Esin

doi:10.1016/j.jpedsurg.2004.05.014

Cited by 24 publications

(37 citation statements)

References 19 publications

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“…These findings suggest that trapidil reduces lipid peroxidation and free oxygen radicals in the injured tissue. Reactive oxygen radicals, NO, endothelin were found to increase in the early phase of corrosive esophagitis in an experimental study by Ozel et al (2004). We found that NO levels significantly increase in the untreated group and significantly decrease by trapidil in the treated group.…”

Section: Discussionmentioning

confidence: 52%

“…It was also reported in experimental model that estradiol and progesteron, ketotifen, Caffeicacid Phenyl Ester (CAPE), Epidermoid Growth Factor (EGF) inhibited collagen synthesis, but, these have not been used in clinic (Demirbilek et al 1994;Koltuksuz et al 2001;Yukselen et al 2004). Very few studies were found to investigate the acute phase of corrosive esophagitis (Gunel et al 1999;Ozel et al 2004). Gunel et al (1999) showed that tissue oxygen radicals were significantly increased within first 24 hour in corrosive esophageal injury.…”

Section: Discussionmentioning

confidence: 99%

“…NO can cause mucosal hyperemia with vasodilatation and leucocytic translocation to the extra vascular area and increase tissue edema (Whittle 1994). Ozel et al (2004) suggested that leucocytes, vascular endothelium, free oxygen radicals, NO and endothelin may be responsible for the early effects on the corrosive injury of esophagus.…”

Section: Discussionmentioning

confidence: 99%

“…Depth of the ulcer and polymorphonuclear leukocyte (pmn) infiltration were evaluated. The injury was graded according to the depth of the ulcer as grade 0 (no ulcer); grade I (ulcer involving only the epithelium); grade II (ulcer involving epithelium and muscularis mucosa); grade III (ulcer proceeding to submucosa); and grade IV (ulcer proceeding to inner circular muscular layer and full thickness ulcer development) (Ozel et al 2004). …”

Section: Histopathologic Studymentioning

confidence: 99%

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Trapidil, an Inhibitor for Phosphodiesterase and Platelet-Derived-Growth Factor, Ameliorates Corrosive Esophageal Burn in Rats

Somuncu

Çakmak

Erdoğan

et al. 2005

Tohoku J. Exp. Med.

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burn is a common health problem in the pediatric age group and causes serious esophageal injuries. The medical treatment in acute phase of corrosive esophageal injury is of particular importance for prevention of esophageal stricture. We therefore aimed to investigate the possible beneficial effect of trapidil (triazolopyrimidine), an inhibitor for phosphodiesterase and platelet-derived-growth-factor, during acute phase of esophageal corrosive injury. Wistar albino rats were randomly allocated to untreated, treated, and sham-operated groups (n = 10 for each group). Corrosive esophageal burn was generated with 10% NaOH solution. The rats were left untreated (untreated group) or treated with trapidil as a single dose of 40 mg/kg intraperitoneally after one hour of the injury (treated group). Abdominal esophageal segment was isolated and tied in sham-control group. The studied esophageal segment was removed from each animal after 24 hours. Malondialdehyde (MDA) and nitric oxide (NO) levels were measured in the esophageal tissues. The ulcer depth was graded by histopathologic examination. MDA and NO levels were significantly higher in the untreated group than in the treated group. Namely, trapidil treatment significantly decreased MDA and NO levels in the injured tissues, the levels of which are similar to those in the tissues of control animals. The grades of ulcer depth were significantly improved in the treated group. These results indicate that the reactive oxygen radicals increase in the early phase of corrosive esophagitis and cause tissue damage. We suggest that trapidil treatment may be useful in acute phase of corrosive esophageal injury. corrosive esophagitis; free oxgen radicals; trapidil; triazolopyrimidine © 2005 Tohoku University Medical PressInfants and young children often accidentally ingest corrosive substances. Ingestion of corrosive substance is a common health problem in the pediatric age group. Accidental ingestion of corrosive substances leads to serious upper aero digestive and esophageal injuries. Corrosive esophageal burns are the most common cause of esophageal stricture development (Haller et al.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Histopathologic Studymentioning

confidence: 99%

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Trapidil, an Inhibitor for Phosphodiesterase and Platelet-Derived-Growth Factor, Ameliorates Corrosive Esophageal Burn in Rats

Somuncu

Çakmak

Erdoğan

et al. 2005

Tohoku J. Exp. Med.

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“…Additionally, an increase in free oxygen radicals (FORs) leads to tissue damage during the early phase of corrosive esophageal injury. 3,7,8 Fibrosis, which is characterized by severe inflammation and increased collagen deposition, occurs due to this tissue damage. Interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) are known as pro-inflammatory cytokines (PICs).…”

mentioning

confidence: 99%

Late Complications of Corrosive Esophagitis May Be Predicted Using Biochemical Methods: Preliminary Report

Temız¹,

Unal²,

Koçer³

et al. 2012

Turkiye Klinikleri J Med Sci

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A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : High tissue levels of free oxygen radicals (FORs) have been reported in corrosive esophagitis (CE). The present study aimed to determine whether prediction of injury and late complications in CE is possible based on blood levels of FORs, Interleukin (IL)-6, Tumor necrosis factor (TNF)-α, and IL-10. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Forty-two male Wistar albino rats were randomly divided into 2 main groups (A and B), each consisting of 3 subgroups (A1, A2, A3, B1, B2, and B3) of equal number. To induce experimental CE 17.5% NaOH was used in subgroups A2 and B2, and 37.5% NaOH in subgroups A3 and B3. The sham groups (A1 and B1) received 0.9% NaCl. Blood levels of lipid hydroperoxide (LPO) and glutathione peroxidase (GPx) were measured in group A. Blood levels of IL-6, IL-10, and TNF-α were measured in group B. Esophageal samples for histopathological evaluation were obtained following scarification after 3 weeks. R Re es su ul lt ts s: : There were significant differences between the sham subgroups and CE subgroups, regarding histopathological scores (p< 0.05). There was no significant difference between the groups in blood levels of LPO, GPX, TNF-α or IL-10 (p> 0.05). There were significant differences between severe CE subgroups and the sham subgroups, based on IL-6 values (p= 0,007). C Co on nc cl lu us si io on n: : In contrast to previous reports, which demonstrated increased tissue levels of FORs, blood levels of FORs were not high in this study. Nonetheless, in the light of the observed differences in IL-6 levels between the subgroups, we suggest that injury and late complications of CE may be predicted following additional experimental and clinical research using biochemical methods.K Ke ey y W Wo or rd ds s: : Caustics; esophagitis; complications; cytokines Ö ÖZ ZE ET T A Am ma aç ç: : Koroziv özofajitte, yaralanmanın ve geç komplikasyonların öngörülmesinde çeşitli yöntem-ler bildirilmiştir. Yapılan çalışmalar, akut dönemde, dokudaki serbest oksijen radikalleri (SOR) düzeyleri-nin artmış olduğunu ortaya koymuştur. Çeşitli travmalarda interlökin (IL)-6, tümör nekroz faktörü (TNF)-α ve IL-10 gibi proenflamatuar sitokin (PES) ve anti-enflamatuar sitokin (AES) düzeylerinde değişiklikler olduğu bilinmektedir. Bu bilgiler ışığında koroziv özofajit modelinde SOR, PES ve AES'lerin kan düzeyleri-nin, doku hasarının ve striktür gelişiminin öngörülmesinde etkili olup olamayacağının araştırılması amaçlanmıştır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Çalışmada 42 erkek Wistar Albino sıçan kullanıldı. Sıçanlar, A ve B grupları şeklinde iki ana gruba ayrıldıktan sonra, bu iki ana grup da her birinde eşit sayıda sıçan olacak şekilde üçer alt gruba ayrıldı (A1, A2, A3, B1, B2 ve B3). A2 (n= 7) ve B2 (n= 7) gruplarında %17,5'luk sodyum hidroksit (NaOH), A3 (n= 7) ve B3 (n= 7) gruplarında ise %37,5'luk NaOH kullanılarak özofajit oluşturuldu. Kontrol gruplarını oluşturan A1 (n= 7) ve B1 (n= 7) gruplarında izotonik kullanıldı. A g...

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Role of allopurinol on oxidative stress in caustic burn: cure for stricture?

et al. 2007

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An experimental study was conducted to investigate the effects of allopurinol, which inhibits the enzyme xanthine oxidase, on oxidative stress and on the prevention of stricture development after esophageal caustic injuries in rat. A randomized controlled study was conducted and 60 Wistar albino rats were divided into 6 equal groups, three groups for the acute phase and 3 groups for the chronic phase. Caustic esophageal burn was created by application of 37.5% NaOH to the distal esophagus. Allopurinol was administered at 40 mg/kg daily. Efficacy of the treatment for the acute phase was assessed by measuring tissue malondialdehyde (MDA), nitric oxide (NO) and glutathione (GSH) at the 3rd day; and for the chronic phase by determining tissue hydroxyproline content and histopathologic damage score at the 28th day. We found an increase in XO, MDA and GSH levels and a decrease in NO levels in the acute phase. Allopurinol reinstated the increase in XO significantly, while MDA, GSH and NO levels were reinstated insignificantly. There was no significant difference in means of tissue hydroxyproline content. Histopathologic damage scores were significantly lower in the allopurinol treated group. This study, which is to our knowledge, the first in the literature investigating the influence of allopurinol on caustic esophageal burn, reveals that allopurinol effects MDA, GSH and NO levels insignificantly in the acute phase of caustic esophageal burn and decreases fibrosis significantly in the chronic phase.

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The roles of free oxygen radicals, nitric oxide, and endothelin in caustic injury of rat esophagus

Cited by 24 publications

References 19 publications

Trapidil, an Inhibitor for Phosphodiesterase and Platelet-Derived-Growth Factor, Ameliorates Corrosive Esophageal Burn in Rats

Trapidil, an Inhibitor for Phosphodiesterase and Platelet-Derived-Growth Factor, Ameliorates Corrosive Esophageal Burn in Rats

Late Complications of Corrosive Esophagitis May Be Predicted Using Biochemical Methods: Preliminary Report

Role of allopurinol on oxidative stress in caustic burn: cure for stricture?

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