The Role of Vitamin C on the Structural Changes of Male Albino Rats Kidney Induced by Tramadol

Abstract

Aim of study: The aim of this work is to detect the toxic effects of tramadol on the microscopic structure of the kidneys of adult male albino rats, and the possible protective role of vitamin C against these toxic effects. Method: Thirty adult albino rats are used in this study. The animals were divided into three equal groups: Group A (control group): Animals were injected daily with normal saline for 4 weeks. Group B (Tramadol treated group) (TGI): Animals were injected daily with tramadol in a dose of 50mg/kg b.wt for 4 weeks. Group C (group treated with tramadol+vitamin C) (TGII): Animals were injected with vitamin C in a dose 100mg/Kg/b.wt half hour prior to tramadol injection in the same dose as in group B for 4 weeks. The injection was given intraperitoneally for all animals. At the end of the experiments, the rats were anaesthetized by ether, then killed. After that kidneys removed, fixed in formaldehyde and processed for microscopic examination. Result: Tramadol causes shrinkage of renal glomeruli with widening of bowman's space. Renal tubules showed hydropic degeneration and vacuolization of their epithelial cells. Infiltration of mononuclear cells along with Hyaline cast is seen within the degenerated tubules. Giving vitamin C prior to tramadol injection results in improvement in the microscopical structure of kidney mainly in glomeruli and tubules. Conclusion: Using tramadol caused damage to renal glomeruli and tubules in renal cortex. The addition of vitamin C partially improved the histological structure of kidney.

Effect of Cadmium Chloride on the Histological Structure of Lung in Adult Male Mice with and without Parsley Oil

“…Oxidative damage is initiated by imbalance between oxidant and antioxidant resulting in mitochondrial damage and DNA damage which are the main manifestation of oxidative stress that occurs in cadmium toxicity [14], [15]. Concomtant use of antioxidants produce improvement in the histological structure of the organ, this finding is in agreement with the finding of other workers [16], [17], [18], [19], [20].…”

The Role of Vitamin C in Amelioration of Hepatorenal Toxicity of Cefotaxime in Adult Albino Rats (Histological Study)

“…The animals which received cefotaxime I.P. with Vitamin C in the third group the hepato-renal changes were seem ameliorated than the second group, the liver cells appeared with less cytoplasmic vacuolation, absence of necrosis, and decreased the congestion and sinusoidal dilation but the inflammatory cells infiltration is still identified in some focal areas while the renal tissue shows normal appearing glomeruli and the eosinophilic material deposition on the bowman capsule was disappeared, the renal tubular epithelium with less degenerative features apart from still inflammatory cells infiltration, these changes were declare the effect of Vitamin C as protective agent against the injurious effects of cefotaxime that shown in the second group, which was proved by other study that shown the hepato-renal protective effects of Vitamin C after using the ceftriaxone [19], [20] and others when used Vitamin C as protective agents against the effect of some drugs or toxic agents [12], [13], [21].…”

“…Vitamin C is a water soluble vitamin which acts as antioxidant that can be used to ameliorate the hepato-renal toxicity induced by different agents [12], [13].…”

Hematological Parameters and Blood Smear Effects of Tramadol on Male Rabbits

“…Co.). The chemical analysis of the pellets [9] showed that they contained 15.8 % crude protein, 11.3 % crude fibre, 3.7 % ether extract, 7.2 % ash, 92.9 % organic matter and 62.4 % nitrogen free extract % as DM basis.The first group was used as control, while, groups 2 was treated with tramadol by gavage at a dose of 50 mg/kg B.W/day (1/50 of tramadol) lethal dose [10]. for 6 successive weeks.…”