The Role of VIP and Somatostatin in the Control of GH and Prolactin Release in Anorexia Nervosa and in Obesity

Baranowska, B; Radzikowska, M.; Wasilewska-Dziubińska, Elżbieta; Roguski, Krzysztof; Borowiec, Monika

doi:10.1111/j.1749-6632.2000.tb07013.x

Cited by 7 publications

(2 citation statements)

References 27 publications

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“…17 Activated VPACR-1 and VPACR-2 increase intracellular calcium signaling in hypothalamic neurons containing neuropeptide Y. 18 Plasma VIP is notably reduced in obese women compared to lean women, 19 suggesting that the VIP signaling pathway through VPAC2 receptors might be playing an important role in the development of overweight and eventually obesity. 20 This study focuses on comparing lean and obese N. alstoni females for the expression of the leptin, ghrelin, and VPAC2 receptors in the hypothalamus as well as their plasma ghrelin concentration under two conditions: feeding ad libitum and 24-hour fasting.…”

Section: Introductionmentioning

confidence: 99%

Hypothalamic expression of anorexigenic and orexigenic hormone receptors in obese femalesNeotomodon alstoni:Effect of fasting

Báez-Ruíz

Luna-Moreno²,

Carmona-Castro³

et al. 2013

Nutritional Neuroscience

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Obesity is a world problem that requires a better understanding of its physiological and genetic basis, as well as the mechanisms by which the hypothalamus controls feeding behavior. The volcano mouse Neotomodon alstoni develops obesity in captivity when fed with regular chow diet, providing a novel model for the study of obesity. Females develop obesity more often than males; therefore, in this study, we analysed in females, in proestrous lean and obese, the differences in hypothalamus expression of receptors for leptin, ghrelin (growth hormone secretagogue receptor GHS-R), and VPAC, and correlates for plasma levels of total ghrelin. The main comparisons are between mice fed ad libitum and mice after 24 hours of fasting. Mice above 65 g body weight were considered obese, based on behavioral and physiological parameters such as food intake, plasma free fatty acids, and glucose tolerance. Hypothalamic tissue from obese and lean mice was analysed by western blot. Our results indicate that after ad libitum food access, obese mice show no significant differences in hypothalamic leptin receptors, but a significant increase of 60% in the GHS-R, and a nearly 62% decrease in VPAC2 was noted. After a 24-hour fast, plasma ghrelin increased nearly two fold in both lean and obese mice; increases of hypothalamic leptin receptors and GHS-R were also noted, while VPAC2 did not change significantly; levels of plasma free fatty acids were 50% less after fasting in obese than in lean animals. Our results indicate that in obese N. alstoni mice, the levels of orexigenic receptors in the hypothalamus correlate with overfeeding, and the fact that lean and obese females respond in different ways to a metabolic demand such as a 24-hour fast.

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Section: Introductionmentioning

confidence: 99%

Hypothalamic expression of anorexigenic and orexigenic hormone receptors in obese femalesNeotomodon alstoni:Effect of fasting

Báez-Ruíz

Luna-Moreno²,

Carmona-Castro³

et al. 2013

Nutritional Neuroscience

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“…Blood levels of VIP are low in obese women and high in patients with anorexia nervosa, which indicates that VIP is involved in PRL/GH secretory dysfunction in these 2 diseases [24].…”

Section: Physiological Activitiesmentioning

confidence: 99%

Vasoactive Intestinal Peptide (VIP) and VIP Receptors-Elucidation of Structure and Function for Therapeutic Applications

Igarashi¹,

Fujimori²,

Ito³

et al. 2011

IJCM

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Preparation of Human Serum for Prolactin Measurement by Multiple Reaction Monitoring Mass Spectrometry

Waybright

Faupel-Badger

et al. 2013

Methods in Molecular Biology

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The measurement of the protein hormone prolactin (PRL) in biological samples has developed over the years into a routine clinical assay aiding the diagnosis of multiple medical conditions. PRL is known to exist in multiple isoforms circulating throughout the body. Current methodologies for measuring the PRL levels typically involve a variety of immunoassays. However, most of these tests are not capable of distinguishing between the different isoforms. To address this need, we have developed a highly specialized method employing multiple reaction monitoring mass spectrometry (MRM-MS) capable of monitoring seven distinct peptides from two of the most common prolactin isoforms (the 23 kDa PRL and its 16 kDa N-terminal cleavage product). Since serum is the main source of clinical specimen for the measurement of prolactin isoforms, the method described in this chapter is focused on the approach to processing whole serum samples for prolactin analysis via reversed-phase liquid chromatography (RPLC) and MRM-MS.

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The Role of VIP and Somatostatin in the Control of GH and Prolactin Release in Anorexia Nervosa and in Obesity

Abstract: MATERIAL AND METHODSThe patients consisted of 30 anorectic women (AN) aged 16-29 years (mean 22 years), 23 obese women aged 19-33 years (mean 29 years), and 25 lean women of the control group aged 25-29 years (mean 26 years).

Cited by 7 publications

References 27 publications

Hypothalamic expression of anorexigenic and orexigenic hormone receptors in obese femalesNeotomodon alstoni:Effect of fasting

Hypothalamic expression of anorexigenic and orexigenic hormone receptors in obese femalesNeotomodon alstoni:Effect of fasting

Vasoactive Intestinal Peptide (VIP) and VIP Receptors-Elucidation of Structure and Function for Therapeutic Applications

Preparation of Human Serum for Prolactin Measurement by Multiple Reaction Monitoring Mass Spectrometry

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