The role of vascular endothelial growth factor and mesenchymal stem cells during angiogenesis

Oki, Nanae; Abe, Takaharu; Kunimatsu, Ryo; Sumi, Keisuke; Awada, Tetsuya; Tsuka, Yuji; Nakajima, Kengo; Ando, Kazuyo; Tanimoto, Kotaro

doi:10.4066/biomedicalresearch.29-18-887

Cited by 4 publications

(4 citation statements)

References 26 publications

(30 reference statements)

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“…As the immobilized VEGF content increases, the number and length of the lumen-like structures raises as well. However, one previous study conducted by Oki et al [49] investigated the effect of soluble VEGF on the formation of HUVEC tubular structures and showed that lumens were not formed in systems subjected to soluble VEGF 50 ng ml −1 . However, in our study by immobilizing VEGF on the sheets, we observed the lumen-like structures of HUVECs.…”

Section: Formation Of Lumen-like Structuresmentioning

confidence: 97%

Retracted: Induced cell migration based on a bioactive hydrogel sheet combined with a perfused microfluidic system

Jafarkhani¹,

Salehi²,

Mashayekhan³

et al. 2020

Biomed. Mater.

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Endothelial cell migration is a crucial step in the procedure of new blood vessel formation-a necessary process to maintain cell viability inside thick tissue constructs. Here, we report a new method for maintaining cell viability and inducing cell migration using a perfused microfluidic platform based on collagen gel and a gradient hydrogel sheet. Due to the helpful role of the extracellular matrix (ECM) components in cell viability, we developed a hydrogel sheet from decellularized tissue (DT) of the bovine heart and chitosan (CS). The results showed that the hydrogel sheets with an optimum weight ratio of CS/DT=2 possess a porosity of around 75%, a mechanical strength of 23 kPa, and display cell viability up to 78%. Then, we immobilized a radial gradient of vascular endothelial growth factor (VEGF) on the hydrogel sheet to promote human umbilical vein endothelial cells (HUVECs) migration. Finally, we incorporated the whole system as an entirety on the top of the microfluidic platform and studied cell migration through the hydrogel sheet in the presence of soluble and immobilized VEGF. The results demonstrated that immobilized VEGF stimulated cell migration in the hydrogel sheet at all depths compared with soluble VEGF. The results also showed that applying a VEGF gradient in both soluble and immobilized states had a significant effect on cell migration at limited depths (<100 μm). The main finding of this study is a significant improvement in cell migration using an in vivo imitating, costefficient, and highly reproducible platform, which may open up a new perspective for tissue engineering applications.

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Section: Formation Of Lumen-like Structuresmentioning

confidence: 97%

Retracted: Induced cell migration based on a bioactive hydrogel sheet combined with a perfused microfluidic system

Jafarkhani¹,

Salehi²,

Mashayekhan³

et al. 2020

Biomed. Mater.

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“…MSCs have been used in many studies due to their abilities to promote vessel formation and maturation by different mechanisms ( Navone et al, 2014 ; Oki et al, 2018 ). The MSCs produce pro-angiogenic cytokines such as hypoxia-inducible factor 1α (HIF-1α), angiogenin, angiopoietin I, angiopoietin II, angiopoietin IV, interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), insulin-like growth factor 1 (IGF-1), VEGF, bFGF, PDGF, transforming growth factor-β (TGF-β), monocyte chemoattractant protein-1 (MCP-1), and as well as by triggering the VEGF-A signaling cascade.…”

Section: Supporting Cellsmentioning

confidence: 99%

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

Shafiee

Shariatzadeh

Zafari

et al. 2021

Front. Bioeng. Biotechnol.

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Currently, the fabrication of a functional vascular network to maintain the viability of engineered tissues is a major bottleneck in the way of developing a more advanced engineered construct. Inspired by vasculogenesis during the embryonic period, the in vitro prevascularization strategies have focused on optimizing communications and interactions of cells, biomaterial and culture conditions to develop a capillary-like network to tackle the aforementioned issue. Many of these studies employ a combination of endothelial lineage cells and supporting cells such as mesenchymal stem cells, fibroblasts, and perivascular cells to create a lumenized endothelial network. These supporting cells are necessary for the stabilization of the newly developed endothelial network. Moreover, to optimize endothelial network development without impairing biomechanical properties of scaffolds or differentiation of target tissue cells, several other factors, including target tissue, endothelial cell origins, the choice of supporting cell, culture condition, incorporated pro-angiogenic factors, and choice of biomaterial must be taken into account. The prevascularization method can also influence the endothelial lineage cell/supporting cell co-culture system to vascularize the bioengineered constructs. This review aims to investigate the recent advances on standard cells used in in vitro prevascularization methods, their co-culture systems, and conditions in which they form an organized and functional vascular network.

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“…Transplanted MSCs can contribute to bone regeneration through angiogenesis stimulation [ 242 ]. hMSCs reside in hypoxic perivascular niches [ 243 ], express HIF-1α in response to hypoxic condition in defect site [ 244 – 247 ], and induce the expression of angiogenic factors such as VEGF, TGF-β, SDF-1, and stem cell factor (SCF) [ 248 ]. The studies showed that VEGF plays an important role in neovascularization and angiogenesis during the development of most tissues including bone [ 249 , 250 ].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal stem cells: amazing remedies for bone and cartilage defects

et al. 2020

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Skeletal disorders are among the leading debilitating factors affecting millions of people worldwide. The use of stem cells for tissue repair has raised many promises in various medical fields, including skeletal disorders. Mesenchymal stem cells (MSCs) are multipotent stromal cells with mesodermal and neural crest origin. These cells are one of the most attractive candidates in regenerative medicine, and their use could be helpful in repairing and regeneration of skeletal disorders through several mechanisms including homing, angiogenesis, differentiation, and response to inflammatory condition. The most widely studied sources of MSCs are bone marrow (BM), adipose tissue, muscle, umbilical cord (UC), umbilical cord blood (UCB), placenta (PL), Wharton’s jelly (WJ), and amniotic fluid. These cells are capable of differentiating into osteoblasts, chondrocytes, adipocytes, and myocytes in vitro. MSCs obtained from various sources have diverse capabilities of secreting many different cytokines, growth factors, and chemokines. It is believed that the salutary effects of MSCs from different sources are not alike in terms of repairing or reformation of injured skeletal tissues. Accordingly, differential identification of MSCs’ secretome enables us to make optimal choices in skeletal disorders considering various sources. This review discusses and compares the therapeutic abilities of MSCs from different sources for bone and cartilage diseases.

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The role of vascular endothelial growth factor and mesenchymal stem cells during angiogenesis

Cited by 4 publications

References 26 publications

Retracted: Induced cell migration based on a bioactive hydrogel sheet combined with a perfused microfluidic system

Retracted: Induced cell migration based on a bioactive hydrogel sheet combined with a perfused microfluidic system

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

Mesenchymal stem cells: amazing remedies for bone and cartilage defects

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