The role of tumor necrosis factor (TNF)-? in the antitumor effect of intrapleural injection of Lactobacillus casei strain Shirota in mice

Abstract

The involvement of several cytokines in the antitumor effect induced by intrapleural (i.pl.) injection of heat-killed cells of Lactobacillus casei strain Shirota (LC 9018) in mice was investigated. Injection of LC 9018 i.pl. into Meth A fibrosarcoma (Meth A)-bearing mice not only significantly prolonged the survival of the mice, but also effectively inhibited the accumulation of malignant pleural fluid in the thoracic cavity. In the thoracic cavity of tumor-bearing mice treated with LC 9018, we observed large amounts of several cytokines including interleukin (IL)-1beta, interferon (IFN)-gamma, IL-12 and tumor necrosis factor (TNF)-alpha. Both anti-IFN-gamma and anti-IL-12 monoclonal antibody (mAb) treatments partially diminished the antitumor activity of LC 9018 in vivo, while the treatment of anti-IL-1beta mAb did not influence the survival of the mice. However, anti-TNF-alpha mAb treatment completely abolished the antitumor effect of LC 9018 in vivo, suggesting that in this model LC 9018 has a survival-prolonging effect involving certain cytokines. Moreover, i.pl. injection of mouse recombinant TNF-alpha into Meth A-bearing mice pretreated with anti-TNF-alpha mAb partially restored the survival-enhancing effect of LC 9018. These results led us to conclude that TNF-alpha induced by i.pl. injection of LC 9018 plays an important role in the antitumor effect of LC 9018 in vivo.

S-Layer Protein Mediates the Stimulatory Effect of Lactobacillus helveticus MIMLh5 on Innate Immunity

“…Nonetheless, such an ability of LAB should not be considered detrimental. In fact, even though cytokines belonging to the TNF-␣ superfamily are connected to the occurrence of inflammatory diseases (54), they have also been shown to participate in the rejection of tumors and infections (55)(56)(57). Furthermore, the induction of TNF-␣ could be important for the initiation of cross talk among immune cells without causing any inflammation or detrimental effects (30).…”

Role of sortase-dependent pili ofBifidobacterium bifidumPRL2010 in modulating bacterium–host interactions

“…Triggering of increased TNF-α production by pili encoded by B. bifidum PRL2010 may be an interesting feature of this species as one of the first colonizers of the human gut (3). In fact, cytokines belonging to the TNF-α superfamily are not only linked to the occurrence of inflammatory diseases (45), but also play a role in the rejection of tumors and the response to infections (46,47). In addition, the induction of TNF-α may be important for the initiation of cross-talk among immune cells without causing any inflammation or detrimental effects (43).…”

Physiological Characteristics and Immunomodulating Activity by Lactobacillus paracasei subsp. paracasei BFI46 Isolated from New-Born Infant Feces

“…Animal studies showed that LAB exerts antitumor effects. Some LAB, such as Lactobacillus rhamnosus GG (Goldin et al, 1996), Lactobacillus acidophilus (Goldin and Gorbach, 1977;Lidbeck et al, 1992), Bifidobacterium longum (Reddy and Rivenson, 1993), Lactobacillus casei strain Shirota (Yasutake et al, 2000) and components of LAB (Shiomi et al, 1982) were shown to exert tumor-suppressing effects. For reduction of allergy, Matsuzaki et al (1998) reported that oral feeding of Lactobacillus casei strain Shirota effectively inhibited Immunoglobulin(Ig)E production both in vivo and in vitro.…”