Physiological Characteristics and Immunomodulating Activity by Lactobacillus paracasei subsp. paracasei BFI46 Isolated from New-Born Infant Feces

Abstract

In order to develop a new starter for fermented milk, Lactobacillus paracasei subsp. paracasei BFI46 (BFI46) obtained from new-born infant feces was investigated for physiological characteristics. Good immunomodulating activity was evident compared with commercial lactic acid bacteria starter cultures. The optimum growth temperature of BFI46 was 40 o C with 12 h required to reach pH 4.3. Testing with 13 different antibiotics revealed greatest sensitivity of BFI46 to penicillin-G and chloramphenicol, and heightened resistance to neomycin, kanamycin and polymyxin. BFI46 displayed higher esterase activities compared to 18 other enzymes, was comparatively tolerant to bile juice and able to survive at pH 2 for 3 h, and displayed high resistance against Escherichia coli and Salmonella Typhimurium with a survival rate of 57.14% and 96.36%, respectively. The results indicate that BFI46 could be an excellent starter culture for fermented milk with high level of immunomodulating activity.

Physiological Characteristics and Immunomodulating Activity of Streptococcus macedonicus LC743 Isolated from Raw Milk

“…The log value of population after 7 h incubation without 0.3% oxgall was 9 but 8.4 with the addition of 0.3% bile so that it has shown good bile tolerance. This result is similar to the report of Lim et al (2010) on the bile tolerance of L. paracasei subsp. paracasei BFI46 that it has shown slight decrease in the log value of population from 9 to 8.5.…”

“…The leucine arylamidase and acid phosphatase activity was 5 and it was relatively high on the other hand β-glucuronidase activity of carcinogen-producing enzymes in the gut was negative and there was no enzyme activity. This result is similar to the report of Lim et al (2010) on the β-glucuronidase activity of L. paracasei subsp. paracasei BFI46.…”

“…S. macedonicus LC743 showed more sensitive to novobiocin and oxacillin compared to 14 different antibiotics, and showed most resistance to gentamycin and lincomycin. Lim et al (2010) has reported that L. paracasei subsp. paracasei BFI46 was sensitive to penicillin-G and chlor- amphenicol but it shown high resistance to neomycin, kanamycin and polymycin.…”

Physiological Characteristics and Production of Vitamin K2by Lactobacillus fermentum LC272 Isolated from Raw Milk

“…The leucine arylamidase and acid phosphatase activity was 5 and it was relatively high on the other hand β-glucuronidase activity one of carcinogen-producing enzymes in the gut was 0 and it was no enzyme activity. This result is similar trends to the report of Lim et al (2010) on the β-glucuronidase activity of L. paracasei subsp. paracasei BFI46.…”

“…L. fermentum LC272 showed more sensitive to rifampicin and penicillin-G in a comparison of 14 different antibiotics, and showed most resistance to streptomycin and gentamycin. Lim et al (2010) has reported that L. paracasei subsp. paracasei BFI46 was sensitive to penicillin-G and chloramphenicol but it shown high resistance to neomycin, kanamycin and polymycin.…”