Physiological Characteristics and Production of Vitamin K<sub>2</sub>by Lactobacillus fermentum LC272 Isolated from Raw Milk

Lim, Sang-Dong; Kim, Kee-Sung; Do, Jeong-Ryong

doi:10.5851/kosfa.2011.31.4.513

Cited by 20 publications

(10 citation statements)

References 26 publications

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“…Using a modified MRS medium, the strain KI62 was isolated from homemade kimchi ( Lim et al, 2011 ). The strain was incubated in Lactobacilli MRS broth (Difco, Detroit, MI, USA) as a growth medium at 37°C for 18 h.…”

Section: Methodsmentioning

confidence: 99%

Physiological Characteristics and Anti-Diabetic Effect of Pediococcus pentosaceus KI62

Kim¹,

Hong²,

Lim³

2021

Food Sci Anim Resour

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The purpose of this study is to examine the physiological characteristics and anti-diabetic effects of Pediococcus pentosaceus KI62. The α-amylase and α-glucosidase inhibitory activity of P. pentosaceus KI62 was 94.86±3.30% and 98.59±0.52%, respectively. In MRS broth containing 3% maltodextrin inoculated by P. pentosaceus KI62, the amounts of short chain fatty acids (SCFA) were propionic acid 18.05±1.85 mg/kg, acetic acid 1.12±0.07 g/100 mL, and butyric acid 2.19±0.061 g/kg, and those of medium chain fatty acids (MCFA) were C8 0.262±0.031 mg/kg, C10 0.279±0.021 mg/kg, and C12 0.203±0.009 mg/kg. Compared to sixteen antibiotics, P. pentosaceus KI62 had the highest sensitivity to penicillin-G and rifampicin, as well as the highest resistance to vancomycin and ampicillin. The strain also showed higher leucine arylamidase and valine arylamidase activities than other enzyme activities, but it did not produce β-glucuronidase which is carcinogenic enzymes. The survival rate of P. pentosaceus KI62 in 0.3% bile was 91.67%. Moreover, the strain showed a 98.63% survival rate in pH 2.0. P. pentosaceus KI62 exhibits resistance to Escherichia coli , Salmonella Typhimurium, Listeria monocytogenes , and Staphylococcus aureus at rates of 29.41%, 38.10%, 51.72%, and 50.47%, respectively. P. pentosaceus (23.31%) showed a similar adhesion ability to L. rhamnosus GG, the positive control (24.49%). These results show that P. pentosaceus KI62 has possibility as a probiotic with anti-diabetic effects.

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Section: Methodsmentioning

confidence: 99%

Physiological Characteristics and Anti-Diabetic Effect of Pediococcus pentosaceus KI62

Kim¹,

Hong²,

Lim³

2021

Food Sci Anim Resour

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“…The strain Q180 was isolated from the feces of healthy adults in a modified MRS medium ( Lim et al ., 2011 ). The strain was incubated in Lactobacilli MRS broth (Difco, USA) as the growth medium at 37℃ for 18 h.…”

Section: Methodsmentioning

confidence: 99%

Physiological Characteristics and Anti-obesity Effect of Lactobacillus plantarum Q180 Isolated from Feces

Park¹,

Cho²,

Kim³

et al. 2014

Korean Journal for Food Science of Animal Resources

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Obesity is strongly associated with several metabolic and chronic diseases and has become a major public health problem of worldwide concern. This study aimed to investigate the physiological characteristics and anti-obesity effects of Lactobacillus plantarum Q180. Lactobacillus plantarum Q180 was isolated from the faces of healthy adults and found to have a lipase inhibitory activity of 83.61±2.32% and inhibited adipocyte differentiation of 3T3-L1 cells (14.63±1.37%) at a concentration of 100 μg/mL. The strain was investigated for its physiological characteristics. The optimum growth temperature of L. plantarum Q180 was 37℃. Lactobacillus plantarum Q180 showed higher sensitivity to novobiocin in a comparison of fifteen different antibiotics and showed the highest resistance to rifampicin, polymyxin B and vancomycin. The strain showed higher β-galactosidase and N-acetyl-β-glucosaminidase activities. It also did not produce carcinogenic enzymes such as β-glucuronidase. The survival rate of L. plantarum Q180 in MRS broth containing 0.3% bile was 97.8%. Moreover, the strain showed a 97.2% survival rate after incubation for 3 h in pH 2.0. Lactobacillus plantarum Q180 was displayed resistance to Escherichia coli, Salmonella Typhimurium and Staphylococcus aureus with rates of 55.6%, 38.0% and 47.6%, respectively. These results demonstrate that L. plantarum Q180 has potential as a probiotic with anti-obesity effects.

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“…L. plantarum Q180 strain was kindly provided by Chong Kun Dang Bio Co. (Ansan, Korea), which was originated from the fecal sample of healthy adult volunteer [21]. The strain was grown in MRS broth (Difco Laboratories INC., Franklin Lakes, NJ) at 37˚C, lyophilized, and then stored at -70˚C until further use.…”

Section: Bacterial Strain and Culture Conditionsmentioning

confidence: 99%

Comprehensive amelioration of high-fat diet-induced metabolic dysfunctions through activation of the PGC-1α pathway by probiotics treatment in mice

et al. 2020

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Although the beneficial effects of probiotics in the prevention or treatment of metabolic disorders have been extensively researched, the precise mechanisms by which probiotics improve metabolic homeostasis are still not clear. Given that probiotics usually exert a comprehensive effect on multiple metabolic disorders, defining a concurrent mechanism underlying the multiple effects is critical to understand the function of probiotics. In this study, we identified the SIRT1-dependent or independent PGC-1α pathways in multiple organs that mediate the protective effects of a strain of Lactobacillus plantarum against high-fat dietinduced adiposity, glucose intolerance, and dyslipidemia. L. plantarum treatment significantly enhanced the expression of SIRT1, PPARα, and PGC-1α in the liver and adipose tissues under HFD-fed condition. L. plantarum treated mice also exhibited significantly increased expressions of genes involved in bile acid synthesis and reverse cholesterol transport in the liver, browning and thermogenesis of adipose tissue, and fatty acid oxidation in the liver and adipose tissue. Additionally, L. plantarum treatment significantly upregulated the expressions of adiponectin in adipose tissue, irisin in skeletal muscle and subcutaneous adipose tissue (SAT), and FGF21 in SAT. These beneficial changes were associated with a significantly improved HFD-induced alteration of gut microbiota. Our findings suggest that the PGC-1α-mediated pathway could be regarded as a potential target in the development of probiotics-based therapies for the prevention and treatment of metabolic disorders.

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Physiological Characteristics and Production of Vitamin K₂by Lactobacillus fermentum LC272 Isolated from Raw Milk

Cited by 20 publications

References 26 publications

Physiological Characteristics and Anti-Diabetic Effect of Pediococcus pentosaceus KI62

Physiological Characteristics and Anti-Diabetic Effect of Pediococcus pentosaceus KI62

Physiological Characteristics and Anti-obesity Effect of Lactobacillus plantarum Q180 Isolated from Feces

Comprehensive amelioration of high-fat diet-induced metabolic dysfunctions through activation of the PGC-1α pathway by probiotics treatment in mice

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Physiological Characteristics and Production of Vitamin K2by Lactobacillus fermentum LC272 Isolated from Raw Milk

Cited by 20 publications

References 26 publications

Physiological Characteristics and Anti-Diabetic Effect of Pediococcus pentosaceus KI62

Physiological Characteristics and Anti-Diabetic Effect of Pediococcus pentosaceus KI62

Physiological Characteristics and Anti-obesity Effect of Lactobacillus plantarum Q180 Isolated from Feces

Comprehensive amelioration of high-fat diet-induced metabolic dysfunctions through activation of the PGC-1α pathway by probiotics treatment in mice

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Product

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Physiological Characteristics and Production of Vitamin K₂by Lactobacillus fermentum LC272 Isolated from Raw Milk