The role of total and high-molecular-weight complex of adiponectin in vascular function in offspring whose parents both had type 2 diabetes

Halperin, Florencia; Beckman, Joshua A.; Patti, Mary Elizabeth; Trujillo, María E.; Garvin, Mona K.; Creager, Mark A.; Scherer, Philipp E.; Goldfine, Allison B.

doi:10.1007/s00125-005-1901-5

Cited by 36 publications

(18 citation statements)

References 66 publications

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“…As described above, we noted a very strong correlation between serum HMW and total adiponectin (r ϭ 0.969), in agreement with a recent study in offspring whose parents both had type 2 diabetes using velocity sedimentation with Western blotting (r ϭ 0.72 between total adiponectin and the HMW-to-total adiponectin ratio) (20). This suggests that total and HMW adiponectin may be regulated synchronously.…”

Section: Discussionsupporting

confidence: 92%

Comparison of Serum High–Molecular Weight (HMW) Adiponectin With Total Adiponectin Concentrations in Type 2 Diabetic Patients With Coronary Artery Disease Using a Novel Enzyme-Linked Immunosorbent Assay to Detect HMW Adiponectin

et al. 2006

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Adiponectin (Acrp30), an adipocyte-derived protein, exists in serum as a trimer, a hexamer, and a high-molecular weight (HMW) form, including 12-18 subunits. Because HMW adiponectin may be biologically active, we measured it in serum using a novel enzyme-linked immunosorbent assay (ELISA) confirmed by gel filtration chromatography that the ELISA detected mainly adiponectin with 12-18 subunits, and we compared HMW with total adiponectin concentration in patients with type 2 diabetes. We next investigated the relationship between serum HMW and coronary artery disease (CAD) in 280 consecutive type 2 diabetic patients, including 59 patients with angiographically confirmed CAD. Total adiponectin was measured in serum by a commercially available ELISA. Like serum total adiponectin, HMW adiponectin correlated positively with HDL cholesterol and negatively with triglyceride, insulin sensitivity, creatinine clearance, and circulating inflammatory markers. Total and HMW adiponectin were significantly higher in women than in men, as was the HMW-tototal adiponectin ratio. Serum HMW and the HMW-to-total adiponectin ratio were significantly lower in men with than without CAD (P < 0.05, respectively). In women, the ratio, but neither total nor HMW adiponectin, tended to be lower when CAD was present. In conclusion, determination of HMW adiponectin, especially relative to total serum adiponectin, is useful for evaluating CAD in type 2 diabetic patients. Diabetes

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Section: Discussionsupporting

confidence: 92%

Comparison of Serum High–Molecular Weight (HMW) Adiponectin With Total Adiponectin Concentrations in Type 2 Diabetic Patients With Coronary Artery Disease Using a Novel Enzyme-Linked Immunosorbent Assay to Detect HMW Adiponectin

et al. 2006

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“…Of the 3 distinct multimeric isoforms in the circulation, HMW adiponectin (12-to 18-mers) is believed to be the most biologically active (5,12,13 ). The HMW adiponectin concentration and/or percentage of HMW adiponectin are reported to be a better index of insulin sensitivity than total adiponectin (14,21 ).…”

Section: Discussionmentioning

confidence: 99%

Analytical Validation and Biological Evaluation of a High–Molecular-Weight Adiponectin ELISA

Sinha¹,

Songer²,

Xiao³

et al. 2007

Clinical Chemistry

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“…However, none of the mentioned gene variants has been clearly associated with changes in plasma adiponectin, and total adiponectin levels have been reported not to be associated with IMT. 22 We cannot exclude that the high-molecular weight isoform of the adipokine, which is believed to carry most of the biological value, 46 may relate to some aspect of vascular structure or function.…”

Section: Discussionmentioning

confidence: 99%

Carotid Intima-Media Thickness in Confirmed Prehypertensive Subjects

Femia¹,

Kozàkovà²,

Nannipieri³

et al. 2007

ATVB

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Objective-The purpose of this study was to test whether carotid intima-media thickness (IMT) is already increased in normotensive subjects who progress to hypertension (confirmed prehypertensives) independently of known determinants of vessel wall thickness. Methods and Results-Common carotid artery (CCA) far-wall IMT was measured (B-mode ultrasound) in 1536 subjects from the population-based Mexico City Diabetes Study at baseline and 3.5 years later. Key Words: blood pressure Ⅲ carotid arteries Ⅲ diabetes mellitus Ⅲ high-resolution B-mode ultrasonography I ntima-media thickness (IMT) of extracranial carotid arteries, as measured by high-resolution B-mode ultrasonography, marks advanced vascular disease in the peripheral and coronary circulation, 1 predicts cardiovascular (CVD) events 2,3 and response to therapy. 4 Several cross-sectional studies have demonstrated the association between carotid IMT and traditional CVD risk factors. 5 Hypertension is a major CVD risk factor that directly contributes to CVD, and carotid IMT is increased in hypertensive patients as compared with normotensive subjects. 6 -8 High blood pressure (BP) plays a direct pathogenic role in the initiation and progression of carotid wall hypertrophy as hemodynamic factors-such as local distending pressure, pulsatile load, and shear stress 9 -12 -can induce intrinsic alterations of the arterial wall resulting in intimal/medial thickening. Previous studies have demonstrated that carotid IMT is increased already in subjects with borderline hypertension. 13,14 This group of subjects is also characterized by concomitant metabolic abnormalities, such as obesityespecially visceral fat accumulation 15 -type 2 diabetes (DM2) or impaired glucose tolerance and dyslipidemia, which enhance CVD risk. Whether carotid IMT is increased also in normotensive subjects that will develop hypertension (ie, confirmed prehypertensives) has not been determined. Therefore, the main aim of this work was to test whether carotid IMT is increased in confirmed prehypertensives independently of known determinants of vessel wall thickness.Low circulating levels of adiponectin, an adipose tissuespecific collagen-like factor, have been reported in association with obesity, 16 DM2, 17 coronary artery disease, and hypertension. 18 Some studies have reported hypoadiponectinemia in subjects with high-normal BP, 19 whereas others have found that only hypertensive patients with insulin resistance 20 or renal dysfunction 21 had lower adiponectin levels. In studies measuring carotid IMT, 22,23 no significant association was detected between adiponectin levels and IMT when accounting for other relevant risk factors. Perhaps more Original

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The role of total and high-molecular-weight complex of adiponectin in vascular function in offspring whose parents both had type 2 diabetes

Cited by 36 publications

References 66 publications

Comparison of Serum High–Molecular Weight (HMW) Adiponectin With Total Adiponectin Concentrations in Type 2 Diabetic Patients With Coronary Artery Disease Using a Novel Enzyme-Linked Immunosorbent Assay to Detect HMW Adiponectin

Comparison of Serum High–Molecular Weight (HMW) Adiponectin With Total Adiponectin Concentrations in Type 2 Diabetic Patients With Coronary Artery Disease Using a Novel Enzyme-Linked Immunosorbent Assay to Detect HMW Adiponectin

Analytical Validation and Biological Evaluation of a High–Molecular-Weight Adiponectin ELISA

Carotid Intima-Media Thickness in Confirmed Prehypertensive Subjects

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