The Role of the Liver Lymphatics in the Transport of Non-Erythroid Bilirubin during Different Stages of Experimental Cholestasis

Bergan, A.; Taksdal, S.; Enge, Ivar

doi:10.1080/00365521.1975.12097014

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…In previous works, researchers proposed that plasma components are filtered through the fenestrae of LSECs into the space of Disse to form the liver lymph, which then flows through the space of Mall and finally drains into LVs in the portal tract [18]. It was reported that the liver lymph contained bilirubin after ligation of the common bile duct [19]. In line with this finding, K. Yamamoto et al found that resin injected into the common bile duct under pressure drains into the hepatic lymphatics [20].…”

Section: Ivyspringmentioning

confidence: 99%

Three-dimensional mapping of hepatic lymphatic vessels and transcriptome profiling of lymphatic endothelial cells in healthy and diseased livers

Huang¹,

Li²,

Liu³

et al. 2023

Theranostics

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Rationale: Hepatic lymphatics are essential for liver homeostasis and immune function. However, the 3D structure and spatial distribution of hepatic lymphatic vessels (LVs) need to be confirmed. Moreover, the molecular information of hepatic lymphatic endothelial cells (LyECs) needs to be further studied. The bottleneck is the lack of specific markers or labeling methods for hepatic lymphatic endothelial cells (LyECs) Methods: Here, we proposed a method for the spatiotemporal sequential injection of antibodies (STSI-Ab) to selectively label hepatic LyECs in vivo. In addition, we also developed an efficient hepatic LyEC sorting method and performed deep transcriptome sequencing on hepatic LyECs. Results: The STSI-Ab method achieved selective labeling of the mouse hepatic lymphatic network. Three-dimensional fluorescence imaging results of the STSI-Ab mouse liver lobe clearly showed that hepatic LVs entangled with the portal vein but were not present in the central vein. The imaging data inspired a novel hepatic lobule structure model with an added set of LVs in the portal area. Furthermore, deep transcriptome sequencing of isolated hepatic LyECs and Masson's trichrome staining results suggested that hepatic LyECs might be an important source of collagen fibers deposited in the portal area during the process of liver fibrosis and bile duct ligation (BDL). Conclusions: We proposed an STSI-Ab method for selectively labeling hepatic LVs, distinguishing the hepatic LVs from other vessels, and mapping their 3D structure. This study opens an avenue for understanding hepatic lymphatic structure and it will be very beneficial to the study of hepatic LyEC functions.

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Section: Ivyspringmentioning

confidence: 99%