The role of the Aedes aegypti Epsilon glutathione transferases in conferring resistance to DDT and pyrethroid insecticides

Lumjuan, Nongkran; Rajatileka, Shavanthi; Changsom, Donch; Wicheer, Jureeporn; Leelapat, Posri; Prapanthadara, La-aied; Somboon, Pradya; Lycett, Gareth; Ranson, Hilary

doi:10.1016/j.ibmb.2010.12.005

Cited by 249 publications

(204 citation statements)

References 25 publications

(33 reference statements)

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“…The majority of reports on insect GSTs have concentrated on their roles in insecticide resistance via the detoxification of insecticides (Enayati et al 2005;Lumjuan et al 2011;Yang et al 2009;Che-Mendoza et al 2009). GSTs confer resistance by detoxifying lipid peroxidation products induced by pyrethroids, thereby protecting tissues from oxidative damage (Vontas et al 2001), as well as by binding pyrethroid molecules in a sequestration mechanism, thereby offering passive protection (Kostaropoulos et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Identification, genomic organization, and oxidative stress response of a sigma class glutathione S-transferase gene (AccGSTS1) in the honey bee, Apis cerana cerana

Yan

Jia

Gao

et al. 2013

Cell Stress and Chaperones

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Glutathione S-transferases (GSTs) are members of a multifunctional antioxidant enzyme superfamily that play pivotal roles in both detoxification and protection against oxidative damage caused by reactive oxygen species. In this study, a complementary DNA (cDNA) encoding a sigma class GST was identified in the Chinese honey bee, Apis cerana cerana (AccGSTS1). AccGSTS1 was constitutively expressed in all tissues of adult worker bees, including the brain, fat body, epidermis, muscle, and midgut, with particularly robust transcription in the fat body. Relative messenger RNA expression levels of AccGSTS1 at different developmental stages varied, with the highest levels of expression observed in adults. The potential function of AccGSTS1 in cellular defenses against abiotic stresses (cold, heat, UV, H 2 O 2 , HgCl 2 , and insecticides) was investigated. AccGSTS1 was significantly upregulated in response to all of the treatment conditions examined, although the induction levels were varied. Recombinant AccGSTS1 protein showed characteristic glutathione-conjugating catalytic activity toward 1-chloro-2,4-dinitrobenzene. Functional assays revealed that AccGSTS1 could remove H 2 O 2 , thereby protecting DNA from oxidative damage. Escherichia coli overexpressing AccGSTS1 showed long-term resistance under conditions of oxidative stress. Together, these results suggest that AccGSTS1 is a crucial antioxidant enzyme involved in cellular antioxidant defenses and honey bee survival.

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Section: Introductionmentioning

confidence: 99%

Identification, genomic organization, and oxidative stress response of a sigma class glutathione S-transferase gene (AccGSTS1) in the honey bee, Apis cerana cerana

Yan

Jia

Gao

et al. 2013

Cell Stress and Chaperones

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“…In populations of A. aegypti from Latin America and Southeast Asia, the mutations Val1016Ile, Val1016Gly, Phe1534Cys and Asp1794Tyr, all in the IIS6 and IIIS6 segment, are correlated with insecticide resistance (BRENGUES et al 2003, CHANG et al 2009, HARRIS et al 2010, LINSS et al 2014. However, only one of these, a valine to isoleucine substitution at codon 1016, has been clearly linked to insecticide resistance; selection pressure under laboratory conditions, bioassays with adults, biochemical assays and molecular screening have confirmed this finding (RODPRADIT et al 2005, STRODE et al 2008, GARCÍA et al 2009, MARTINS et al 2009, LUMJUAN et al 2011, MARCOMBE et al 2012.…”

mentioning

confidence: 99%

Insecticide resistance and genetic variability in natural populations of Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Colombia

Aguirre-Obando¹,

Bona²,

Duque³

et al. 2015

Zoologia (Curitiba)

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“…Metabolic resistance occurs via increases in either protein levels or the activity of detoxification enzymes that resist insecticides. For example, esterases (Wu et al 2011), glutathione S-transferases (Lumjuan et al 2011), and cytochrome P450 (CYP or P450; Edi et al 2014) have all been demonstrated to be associated with metabolic resistance. In addition, insecticides induce physiological changes in mosquitoes such as a thickening of the epidermis, which subsequently lowers their permeability to insecticides (Wood et al 2010).…”

mentioning

confidence: 99%

Venom allergen 5is Associated With Deltamethrin Resistance inCulex pipiens pallens(Diptera: Culicidae)

Lei

Hong

et al. 2015

J Med Entomol

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The mosquito, Culex pipiens pallens (L.), is an important vector of encephalitis and filariasis in northern China. The control of these mosquitoes occurs primarily via the use of pyrethroid insecticides, such as deltamethrin. The widespread and improper application of pyrethroid has resulted in the evolution of pyrethroid resistance amongst many mosquito populations, including Cx. pipiens pallens. Previous studies using high-throughput transcriptome sequencing have identified that the venom allergen 5 gene is differentially expressed between deltamethrin-susceptible and deltamethrin-resistant Cx. pipiens pallens. In this study, quantitative real-time polymerase chain reaction analyses revealed that venom allergen 5 was significantly overexpressed in adult females of both deltamethrin-resistant laboratory populations and two field populations. The transcriptional level of venom allergen 5 in the laboratory populations was elevated as the levels of deltamethrin resistance increased. Full-length cDNAs of the venom allergen 5 gene were cloned from Cx. pipiens pallens, and contained an open reading frame of 765 bp, encoding a protein with 254 amino acids. The deduced amino acid sequence shared 100% identity with the ortholog in Culex quinquefasciatus Say. The overexpression of venom allergen 5 decreased the susceptibility of mosquito cells to deltamethrin, while knockdown of this gene by RNAi increased the susceptibility of mosquitoes to deltamethrin. This study provides the first evidence of the association between the venom allergen 5 gene and deltamethrin resistance in mosquitoes.

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The role of the Aedes aegypti Epsilon glutathione transferases in conferring resistance to DDT and pyrethroid insecticides

Cited by 249 publications

References 25 publications

Identification, genomic organization, and oxidative stress response of a sigma class glutathione S-transferase gene (AccGSTS1) in the honey bee, Apis cerana cerana

Identification, genomic organization, and oxidative stress response of a sigma class glutathione S-transferase gene (AccGSTS1) in the honey bee, Apis cerana cerana

Insecticide resistance and genetic variability in natural populations of Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Colombia

Venom allergen 5is Associated With Deltamethrin Resistance inCulex pipiens pallens(Diptera: Culicidae)

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