The role of tendon derived stem/progenitor cells and extracellular matrix components in the bone tendon junction repair

Qin, Shengnan; Bennett, Samuel; Wang, Wen; Li, Aiguo; Xu, Jiake

doi:10.1016/j.bone.2021.116172

Cited by 18 publications

(17 citation statements)

References 108 publications

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“…It is generally recognized that difficulties in restoring the mechanical properties after BTJ injury are largely due to the failure of fibrocartilage recapitulation[36]. Moreover, in modern RC reconstruction surgeries, anchors fix the tendon to the insertion areas without access to bone marrow, which means BMSCs are not likely the main stem cell source for repair[14, 37, 38].…”

Section: Discussionmentioning

confidence: 99%

Single-cell RNA sequencing reveals cellular and molecular heterogeneity in fibrocartilaginous enthesis formation

Zhang

Wan

Xiao

et al. 2023

Preprint

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The attachment site of the rotator cuff (RC) is a classic fibrocartilaginous enthesis, which is the junction between bone and tendon with typical characteristics of a fibrocartilage transition zone. Enthesis development has historically been studied with lineage tracing of individual genes selected a priori, which does not allow for the determination of single-cell landscapes yielding mature cell types and tissues. Here, we applied Single-cell RNA sequencing (scRNA-seq) to delineate the comprehensive postnatal RC enthesis growth and the temporal atlas from as early as embryonic day 15 up to postnatal week 4. In summary, we compared the development pattern between enthesis and tendon or articular cartilage, then deciphered the cellular heterogeneity and the molecular dynamics during fibrocartilage differentiation. This data provides a transcriptional resource that will support future investigations of enthesis development at the mechanistic level and may shed light on the strategies for enhanced RC healing outcomes.

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Section: Discussionmentioning

confidence: 99%

Single-cell RNA sequencing reveals cellular and molecular heterogeneity in fibrocartilaginous enthesis formation

Zhang

Wan

Xiao

et al. 2023

Preprint

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“…These events might collectively lead to disorganized tendon architecture. Also, recent studies have demonstrated the functional potential of TSPCs to differentiate into fibrocartilage-like cells [ 31 ] to constitute the fibrocartilage transition zone, which played an essential role in the bone-tendon junction [ 32 , 33 ]. In light of our results and these findings, the role of hyperuricemia in TSPCs is of particular importance for ATR patients, particularly during the postrupture recovery process [ 17 , 18 ].…”

Section: Discussionmentioning

confidence: 99%

Asymptomatic Hyperuricemia Is Associated with Achilles Tendon Rupture through Disrupting the Normal Functions of Tendon Stem/Progenitor Cells

Liang

Chen

et al. 2022

Stem Cells International

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Hyperuricemia is a metabolic disorder that is essential to the development of inflammatory gout, with increasing prevalence over recent years. Emerging clinical findings has evidenced remarkable tendon damage in individuals with longstanding asymptomatic hyperuricemia, yet the impact of hyperuricemia on tendon homeostasis and associated repercussions is largely unknown. Here, we investigated whether asymptomatic hyperuricemia was associated with spontaneous ruptures in the Achilles tendon and the pathological effect of hyperuricemia on the tendon stem/progenitor cells (TSPCs). Significantly higher serum uric acid (SUA) levels were found in 648 closed Achilles tendon rupture (ATR) patients comparing to those in 12559 healthy volunteers. In vitro study demonstrated that uric acid (UA) dose dependently reduced rat Achilles TSPC viability, decreased the expressions of tendon collagens, and deformed their structural organization while significantly increased the transcript levels of matrix degradative enzymes and proinflammatory factors. Consistently, marked disruptions in Achilles tendon tissue structural and functional integrity were found in a rat model of hyperuricemia, together with enhanced immune cell infiltration. Transcriptome analysis revealed a significant elevation in genes involved in metabolic stress and tissue degeneration in TSPCs challenged by hyperuricemia. Specifically, reduced activity of the AKT-mTOR pathway with enhanced autophagic signaling was confirmed. Our findings indicate that asymptomatic hyperuricemia may be a predisposition of ATR by impeding the normal functions of TSPCs. This information may provide theoretical and experimental basis for exploring the early prevention and care of ATR.

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“…130 The main advantage of decellularized enthesis scaffolds are that they are easy to obtain and the tissue's biomimetic capacity is guaranteed (especially when the scaffold is based on the same anatomical site). 131,132 A study by Xu et al which developed a porcine decellularized Achilles enthesis scaffold using a new protocol, found that the decellularized enthesis scaffold preserved the typical histological structure of enthesis tissue well (Figure 3(a)). 133 Additionally, their biomechanical assessment showed that the decellularized enthesis scaffold retained mechanical properties that were appropriate for practical applications (Figure 3(b)).…”

Section: The Biological Scaffold Strategymentioning

confidence: 99%

“… 130 The main advantage of decellularized enthesis scaffolds are that they are easy to obtain and the tissue’s biomimetic capacity is guaranteed (especially when the scaffold is based on the same anatomical site). 131 , 132 …”

Section: Tissue Engineering Strategies For Enthesis Reconstructionmentioning

confidence: 99%

Advanced strategies for constructing interfacial tissues of bone and tendon/ligament

et al. 2022

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Enthesis, the interfacial tissue between a tendon/ligament and bone, exhibits a complex histological transition from soft to hard tissue, which significantly complicates its repair and regeneration after injury. Because traditional surgical treatments for enthesis injury are not satisfactory, tissue engineering has emerged as a strategy for improving treatment success. Rapid advances in enthesis tissue engineering have led to the development of several strategies for promoting enthesis tissue regeneration, including biological scaffolds, cells, growth factors, and biophysical modulation. In this review, we discuss recent advances in enthesis tissue engineering, particularly the use of biological scaffolds, as well as perspectives on the future directions in enthesis tissue engineering.

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The role of tendon derived stem/progenitor cells and extracellular matrix components in the bone tendon junction repair

Cited by 18 publications

References 108 publications

Single-cell RNA sequencing reveals cellular and molecular heterogeneity in fibrocartilaginous enthesis formation

Single-cell RNA sequencing reveals cellular and molecular heterogeneity in fibrocartilaginous enthesis formation

Asymptomatic Hyperuricemia Is Associated with Achilles Tendon Rupture through Disrupting the Normal Functions of Tendon Stem/Progenitor Cells

Advanced strategies for constructing interfacial tissues of bone and tendon/ligament

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