The Role of Sugarcane Catalase Gene ScCAT2 in the Defense Response to Pathogen Challenge and Adversity Stress

Sun, Tingting; Liu, Feng; Wang, Wenju; Wang, Zhuqing; Li, Jing; Que, Youxiong; Xu, Liping; Su, Yachun

doi:10.3390/ijms19092686

Cited by 17 publications

(12 citation statements)

References 62 publications

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“…Catalase (CAT) is an iron porphyrin enzyme, which serves as a scavenger of reactive oxygen species (ROS) [37], and CAT related genes have been found to be regulated by biotic and abiotic stresses [38]. For example, the closest homolog in sugarcane, ScCAT2, was found to play a positive role in immune responses during plant–pathogen interactions [39]. The increase of CAT proteins, together with the putative multiprotein bridging factor, makes it tempting to speculate that ROS tolerance mechanisms are activated during ETI interaction.…”

Section: Resultsmentioning

confidence: 99%

Proteomics of PTI and Two ETI Immune Reactions in Potato Leaves

Resjö

Zahid

Burra

et al. 2019

IJMS

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Plants have a variety of ways to defend themselves against pathogens. A commonly used model of the plant immune system is divided into a general response triggered by pathogen-associated molecular patterns (PAMPs), and a specific response triggered by effectors. The first type of response is known as PAMP triggered immunity (PTI), and the second is known as effector-triggered immunity (ETI). To obtain better insight into changes of protein abundance in immunity reactions, we performed a comparative proteomic analysis of a PTI and two different ETI models (relating to Phytophthora infestans) in potato. Several proteins showed higher abundance in all immune reactions, such as a protein annotated as sterol carrier protein 2 that could be interesting since Phytophthora species are sterol auxotrophs. RNA binding proteins also showed altered abundance in the different immune reactions. Furthermore, we identified some PTI-specific changes of protein abundance, such as for example, a glyoxysomal fatty acid beta-oxidation multifunctional protein and a MAR-binding protein. Interestingly, a lysine histone demethylase was decreased in PTI, and that prompted us to also analyze protein methylation in our datasets. The proteins upregulated explicitly in ETI included several catalases. Few proteins were regulated in only one of the ETI interactions. For example, histones were only downregulated in the ETI-Avr2 interaction, and a putative multiprotein bridging factor was only upregulated in the ETI-IpiO interaction. One example of a methylated protein that increased in the ETI interactions was a serine hydroxymethyltransferase.

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Section: Resultsmentioning

confidence: 99%

Proteomics of PTI and Two ETI Immune Reactions in Potato Leaves

Resjö

Zahid

Burra

et al. 2019

IJMS

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“…In addition, lots of stressrelated modified proteins involved in ROS signaling, Ca 2+ signaling, PAL metabolic, glyoxalase system, and hormone metabolism were also identified (Figure 6c and Table S12) and could be induced under cold, drought or S. scitamineum stress (Figure 7 and Table S13). Our group has discovered that an Lascorbate peroxidase 6 gene (APX6), 87 a glyoxalase I gene (SoGloI), 88 a gretchen hagen 3 (ScGH3−1), 89 an allene oxide synthase gene (ShAOS1), 90 two calcineurin B-like protein genes (ScCBL2−1 and ScCBL3−1), 91 two catalase genes (ScCAT1 and ScCAT2), 39,92,93 three thaumatin-like proteins genes (ScTLP1, ScTLP2, and ScTLP3), 94,95 three β-1,3glucanase genes (ScGluA1, ScGluD1, and ScGluD2), 96,97 four chitinase genes (ScChi, ScChiI1, ScChiIV1, and ScChiVII1) 98,99 and the ten CBL-interacting protein kinase genes (ScCIPKs) 100 were involved in sugarcane tolerance to SA, MeJA, ABA, hydrogen peroxide (H…”

Section: ■ Discussionmentioning

confidence: 99%

Deciphering the Atlas of Post-Translational Modification in Sugarcane

Yang

et al. 2023

J. Agric. Food Chem.

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In plants, lysine acetylation (Kac), 2-hydroxyisobutyrylation (Khib), and lysine lactylation (Kla), the three new types of post-translational modification (PTM), play very important roles in growth, development, and resistance to adverse environmental stresses. Herein, we report the first global acetylome, 2-hydroxyisobutyrylome, and lactylome in sugarcane. A total of 8573 Kac, 4637 Khib, and 215 Kla sites across 3903, 1507, and 139 modified proteins were identified. Besides, homology analyses revealed the Kac, Khib, and Kla sites on histones were conserved between sugarcane and rice or poplar. Functional annotations demonstrated that the Kac, Khib, and Kla proteins were mainly involved in energy metabolism. In addition, a number of modified transcription factors and stress-related proteins, which were constitutively expressed in different tissues of sugarcane and induced by drought, cold or Sporisorium scitamineum stress, were identified. Finally, a proposed working mode on how PTM functions in sugarcane was depicted. We thus concluded that PTM should play a role in sugarcane growth, development, and response to biotic and abiotic stresses, but the mechanisms require further investigation. The present study provided the all-new comprehensive profile of proteins Kac, Khib, and Kla and a new perspective to understand the molecular mechanisms of protein PTMs in sugarcane.

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“…CAT2 overexpression led to a higher CAT enzyme activity and enhanced resistance to oxidative stress and pathogen infection in transgenic N. benthamiana plants ( Polidoros et al., 2001 ). Two sugarcane catalase genes, ScCAT1 and ScCAT2 , played a positive role in immune responses in sugarcane- S. scitamineum interactions, as well as in various abiotic stresses ( Su et al., 2014 ; Sun et al., 2018 ). In this study, the expression of CAT1 was also up-regulated and reached the peak of 13.06-fold increase at 1 d post S. scitamineum -inoculation in YT93-159 ( Figure 8E ).…”

Section: Discussionmentioning

confidence: 99%

Genetic identification of SNP markers and candidate genes associated with sugarcane smut resistance using BSR-Seq

Pan

et al. 2022

Front. Plant Sci.

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Sugarcane smut caused by Sporisorium scitamineum is one of the most severe fungal diseases worldwide. In this study, a cross was made between a smut-resistant variety YT93-159 and a smut-susceptible variety ROC22, and 312 progenies were obtained. Two bulks of progenies were then constructed, one consisted of 27 highly smut resistant progenies and the other 24 smut susceptible progenies. Total RNAs of the progenies of each bulk, were pooled and subject to bulked segregant RNA-sequence analysis (BSR-Seq). A total of 164.44 Gb clean data containing 2,341,449 SNPs and 64,999 genes were obtained, 7,295 of which were differentially expressed genes (DEGs). These DEGs were mainly enriched in stress-related metabolic pathways, including carbon metabolism, phenylalanine metabolism, plant hormone signal transduction, glutathione metabolism, and plant-pathogen interactions. Besides, 45,946 high-quality, credible SNPs, a 1.27 Mb region at Saccharum spontaneum chromosome Chr5B (68,904,827 to 70,172,982), and 129 candidate genes were identified to be associated with smut resistance. Among them, twenty-four genes, either encoding key enzymes involved in signaling pathways or being transcription factors, were found to be very closely associated with stress resistance. RT-qPCR analysis demonstrated that they played a positive role in smut resistance. Finally, a potential molecular mechanism of sugarcane and S. scitamineum interaction is depicted that activations of MAPK cascade signaling, ROS signaling, Ca2+ signaling, and PAL metabolic pathway and initiation of the glyoxalase system jointly promote the resistance to S. scitamineum in sugarcane. This study provides potential SNP markers and candidate gene resources for smut resistance breeding in sugarcane.

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The Role of Sugarcane Catalase Gene ScCAT2 in the Defense Response to Pathogen Challenge and Adversity Stress

Cited by 17 publications

References 62 publications

Proteomics of PTI and Two ETI Immune Reactions in Potato Leaves

Proteomics of PTI and Two ETI Immune Reactions in Potato Leaves

Deciphering the Atlas of Post-Translational Modification in Sugarcane

Genetic identification of SNP markers and candidate genes associated with sugarcane smut resistance using BSR-Seq

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