The Role of Staphylococcal Biofilm on the Surface of Implants in Orthopedic Infection

Lu, Y. P.; Han, Pei; Ren, Zun; Han, Pei

doi:10.3390/microorganisms10101909

Cited by 16 publications

(13 citation statements)

References 167 publications

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“…Of note, S. aureus growth itself was not affected by the metal concentrations, suggesting that it might form biofilms in PJIs. ,,− PJIs occur in up to 4% of revision arthroplasties . It was reported that the presence of a foreign body and local metal release reduce the infectivity dose of S.…”

Section: Discussionmentioning

confidence: 98%

Cobalt and Chromium Ions Impair Macrophage Response to Staphylococcus aureus Infection

Tölken,

Wassilew,

Grolimund

et al. 2023

ACS Biomater. Sci. Eng.

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Cobalt−chromium−molybdenum (CoCrMo) alloys are routinely used in arthroplasty. CoCrMo wear particles and ions derived from arthroplasty implants lead to macrophage-driven adverse local tissue reactions, which have been linked to an increased risk of periprosthetic joint infection after revision arthroplasty. While metal-induced cytotoxicity is well characterized in human macrophages, direct effects on their functionality have remained elusive. Synchrotron radiation X-ray microtomography and X-ray fluorescence mapping indicated that peri-implant tissues harvested during aseptic revision of different arthroplasty implants are exposed to Co and Cr in situ. Confocal laser scanning microscopy revealed that macrophage influx is predominant in patient tissue. While in vitro exposure to Cr 3+ had only minor effects on monocytes/macrophage phenotype, pathologic concentrations of Co 2+ significantly impaired both, monocyte/macrophage phenotype and functionality. High concentrations of Co 2+ led to a shift in macrophage subsets and loss of surface markers, including CD14 and CD16. Both Co 2+ and Cr 3+ impaired macrophage responses to Staphylococcus aureus infection, and particularly, Co 2+exposed macrophages showed decreased phagocytic activity. These findings demonstrate the immunosuppressive effects of locally elevated metal ions on the innate immune response and support further investigations, including studies exploring whether Co 2+ and Cr 3+ or CoCrMo alloys per se expose the patients to a higher risk of infections post-revision arthroplasty.

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Section: Discussionmentioning

confidence: 98%

Cobalt and Chromium Ions Impair Macrophage Response to Staphylococcus aureus Infection

Tölken,

Wassilew,

Grolimund

et al. 2023

ACS Biomater. Sci. Eng.

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“…The vast majority of nosocomial infections involved a biofilm-producing organism [ 2 ]. Thus, the development of strategies that limit biofilm development by using modified biomaterials or permitting the dissociation of already-formed biofilms in order to avoid material removal constitute urgent clinical needs in situations of economic or clinical relevance [ 53 ].…”

Section: Biofilm Formation and Clinical Significancementioning

confidence: 99%

Biology and Regulation of Staphylococcal Biofilm

François

Schrenzel

Götz

2023

IJMS

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Despite continuing progress in medical and surgical procedures, staphylococci remain the major Gram-positive bacterial pathogens that cause a wide spectrum of diseases, especially in patients requiring the utilization of indwelling catheters and prosthetic devices implanted temporarily or for prolonged periods of time. Within the genus, if Staphylococcus aureus and S. epidermidis are prevalent species responsible for infections, several coagulase-negative species which are normal components of our microflora also constitute opportunistic pathogens that are able to infect patients. In such a clinical context, staphylococci producing biofilms show an increased resistance to antimicrobials and host immune defenses. Although the biochemical composition of the biofilm matrix has been extensively studied, the regulation of biofilm formation and the factors contributing to its stability and release are currently still being discovered. This review presents and discusses the composition and some regulation elements of biofilm development and describes its clinical importance. Finally, we summarize the numerous and various recent studies that address attempts to destroy an already-formed biofilm within the clinical context as a potential therapeutic strategy to avoid the removal of infected implant material, a critical event for patient convenience and health care costs.

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“…However, implant-associated infection (IAI) is a catastrophic complication of orthopedic surgery. 1 According to statistics, the incidence of IAI ranges from 1 to 30%. 2 Tubercular IAI (TB-IAI) is a rare infection caused by the Mycobacterium tuberculosis complex (MTBC), constituting approximately 0.14% of the cases of tuberculosis (TB).…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Role of Metagenomic Next-Generation Sequencing in Tubercular Orthopedic Implant-Associated Infection

Wang,

et al. 2024

IDR

Self Cite

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The diagnosis of tubercular orthopedic implant-associated infection (TB-IAI) is challenging. This study evaluated the value of metagenomic next-generation sequencing (mNGS) for the diagnosis of TB-IAI and developed a standardized diagnostic procedure for TB-IAI. Methods:The records of all patients with TB-IAI diagnosed and treated at our institution between December 2018 and September 2022 were retrospectively reviewed. Patient demographic characteristics, medical history, laboratory test, microbial culture, histopathology, and mNGS results, and time to diagnosis were recorded. The diagnostic efficiency of mNGS for TB-IAI was assessed by comparing the results and diagnostic time with that of other diagnostic modalities. Results: Ten patients were included in the analysis, including eight with prosthetic joint infections and two with fracture-related infections. The mNGS positivity rate was 100% (10/10), which was higher than that of TB-antibody (11%, 1/9), real-time quantitative polymerase chain reaction (22%, 2/9), T-SPOT.TB (25%, 2/8), purified protein derivative (50%, 4/8), microbial culture (50%, 5/10), and histopathology (20%, 2/10). mNGS shortened the time to diagnosis of TB-IAI. A standardized diagnostic procedure for TB-IAI was developed based on the findings. Conclusion: mNGS is useful for the diagnosis of TB-IAI. mNGS is recommended in cases where it is difficult to identify a pathogen using routine diagnostic tests. The standardized diagnostic procedure might improve TB-IAI diagnosis. Importance: TB-IAI is a rare infection, which occurs after orthopedic surgery and hard to diagnose microbiologically. mNGS is a new detection technique not yet discussed in current literature as a means for TB-IAI diagnostics. Here we describe a cohort of patients with TB-IAI diagnosed by mNGS show high efficiency of mNGS for detection of this pathology and present a clinical algorithm supplementing conventional methods for TB-IAI assessment.

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The Role of Staphylococcal Biofilm on the Surface of Implants in Orthopedic Infection

Cited by 16 publications

References 167 publications

Cobalt and Chromium Ions Impair Macrophage Response to Staphylococcus aureus Infection

Cobalt and Chromium Ions Impair Macrophage Response to Staphylococcus aureus Infection

Biology and Regulation of Staphylococcal Biofilm

Diagnostic Role of Metagenomic Next-Generation Sequencing in Tubercular Orthopedic Implant-Associated Infection

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