The Role of Serum in thi Nutrition Asterococcus Mycoides

Abstract

For grow(,h or A.,yierococcl<S lI1!1coiilc8 in l'Olltpi"x liquid IllNI iml1 , Nl'l'um oould be l'Oplacecl by balancNIll1ix(,ures of a hl)at,-sfahln protein fnwtioll, elioloNiowl, and oleate, when 11 suitahle osmoLie pressllI'o was provided, Olea!!) could I", l'eplaeod by linolcaje 01' by linolollate, and t;he p,'ofein fraction by bovino ""!'tun albumin or by stareh. Both the protein fruction and cholcsto['()l inhibited tlw mpid lysis brought, about by low concentrations ofoloato or otbOl' BUrraGe-active agents. anionic and nOlliOllie. It was concluded that SOl'um provides a nOll-toxie SOllrce of olea!,(), prohably as n moleeulnr uRsociation with protein and cholosterol, lmC tho (Jvidenoo did not inrlieato whothor OJ' not. ehoiesj,ero] iii also required us n. llllj.l'ioni.

Mycoplasma growth factors in bovine serum fraction

“…We have shown that intact lipoprotein is not required for M. pneumoniae and M. arthritidis growth. However, the present investigation has also clearly shown that, besides lipid, some type of serum protein is required, an observation which has been made by other workers for other mycoplasma species (5,21,23,29 Although serum protein is important, it is likely that it plays a mostly passive role as a carrier of cholesterol and fatty acids required for membrane synthesis (5, 17-22, 24, 31, 36), an hypothesis supported by the recent observation of Slutzky et al (27) that cholesterol is transferred from human serum lipoproteins to Acholeplasma laidlawii membranes without apparent degradation or deterioration of the apoprotein moiety. It has also been suggested that serum protein is involved in the regulation of sterol uptake by mycoplasma cells (29,30).…”

“…Culture media for the sterol-requiring mycoplasmas usually contain serum, which supplies protein (21,29,30) and lipid (5, 17-19, 21, 22, 24, 31) essential for in vitro growth. In 1951 Smith and Morton (32), using ammonium sulfate precipitation, prepared a serum fraction later reported to be an alpha-lipoprotein (30), which retained all the growth-supporting activity of serum.…”

Growth‐inhibition Tests With Mycoplasma Mycoides as a Basis for Chemotherapy and Selective Culture Media

“…Sinre M . rnycoides is very sensitive to unsaturated fatty acids and t o saponins (Rodwell, 1956), the poor inhibition in these tests is probably due to the protective effect of the serum (10%) in the RVF-OS medium.…”

The Water Relations of Vibrio Metohnikovi at 30°C

“…The rate of growth of all the Brucella strains was increased by the addition of salts to the basal medium, and two strains of Brucella suis did not grow unless the amount of added sodium chloride was sufficient to reduce the a w to about 0•997. Mager (1955) has reported that the growth of Neisseria perjlava and Pasteurella tularensis was stimulated by various salts and sugars, the optimum concentration in each case corresponding to 0•997-0•995 aw • Rodwell (1956) has shown that the growth of Asterococcus mycoides was sensitive to the tonicity of the medium when serum was replaced by other factors. These few organisms which are osmotically sensitive are also pathogenic to animals, and it may be that the parasitic existence in the closely controlled environment of the host has led to a loss of properties conferring resistance to osmotic change.…”