The role of S-layer in adhesive and immunomodulating properties of probiotic starter culture Lactobacillus brevis D6 isolated from artisanal smoked fresh cheese

Uroić, Ksenija; Novak, Jasna; Hynönen, Ulla; Pietilä, Taija E.; Pavunc, Andreja Leboš; Kant, Ravi; Kos, Blaženka; Palva, Airi; Šušković, Jagoda

doi:10.1016/j.lwt.2016.02.013

Cited by 39 publications

(55 citation statements)

References 35 publications

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“…helveticus MIMLh5 and its SlpA stimulated the innate immune system by inducing proinflammatory mediators such as TNFα and cyclooxygenase 2 (COX-2) in the human macrophage cell line U937 via TLR2 recognition [39]. Similarly, L. brevis Slps induce TNFα in monocytederived dendritic cells (moDC) [46].…”

Section: Discussionmentioning

confidence: 99%

Probiotic Propionibacterium freudenreichii requires SlpB protein to mitigate mucositis induced by chemotherapy

et al. 2019

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Propionibacterium freudenreichii CIRM-BIA 129 (P. freudenreichii wild type, WT) is a probiotic bacterium, which exerts immunomodulatory effects. This strain possesses extractable surface proteins, including SlpB, which are involved in antiinflammatory effect and in adhesion to epithelial cells. We decided to investigate the impact of slpB gene mutation on immunomodulation in vitro and in vivo. In an in vitro assay, P. freudenreichii WT reduced expression of IL-8 (p<0.0001) and TNF-α (p<0.0001) cytokines in LPS-stimulated HT-29 cells. P. freudenreichii ΔslpB, lacking the SlpB protein, failed to do so. Subsequently, both strains were investigated in vivo in a 5-FU-induced mucositis mice model. Mucositis is a common side effect of cytotoxic chemotherapy with 5-FU, characterized by mucosal injury, inflammation, diarrhea, and weight loss. The WT strain prevented weight loss, reduced inflammation and consequently histopathological scores. Furthermore, it regulated key markers, including Claudin-1 (cld1, p<0.0005) and IL-17a (Il17a, p<0.0001) genes, as well as IL-12 (p<0.0001) and IL-1β (p<0.0429) cytokines levels. Mutant strain displayed opposite regulatory effect on cld1 expression and on IL-12 levels. This work emphasizes the importance of SlpB in P. freudenreichii ability to reduce mucositis inflammation. It opens perspectives for the development of probiotic products to decrease side effects of chemotherapy using GRAS bacteria with immunomodulatory surface protein properties.

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Section: Discussionmentioning

confidence: 99%

Probiotic Propionibacterium freudenreichii requires SlpB protein to mitigate mucositis induced by chemotherapy

et al. 2019

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“…They are metabolic expensive products which may be involved in determining cell shape and cell division, but also may act as protective coats, promoters for cell adhesion, molecular sieves, molecule and ion traps, antifouling coatings, virulence factors in pathogenic organisms (Sleytr, Schuster, Egelseer, & Pum, 2014), and as a scaffold for the external display of other proteins or glycoproteins (Klotz, O'Flaherty, Goh, & Barrangou, 2017). Slps have a significant role as adhesins, which interact with different moieties in the intestinal tissue, and as immunomodulators and protective molecules under environmental stressful conditions (Beganović, et al, 2011b;Uroić, et al, 2016). Moreover, Slps can be used as the carriers of antigens or other important molecules, and therefore are good candidates for health-related applications (Hynönen & Palva, 2013).…”

Section: Introductionmentioning

confidence: 99%

Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut

Banić

Uroić

Pavunc

et al. 2018

LWT

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S-layers represent the simplest biological membranes developed during the evolution and are one of the most abundant biopolymers on Earth. Current fundamental and applied research aim to reveal the chemical structure, morphogenesis and function of S-layer proteins (Slps). This is the first paper that describes the Slps of certain Lactobacillus brevis strain isolated from sauerkraut. The whole genome sequence (WGS) analysis of the L. brevis SF9B strain uncovered three genes encoding the putative Slps, but merely one, identified as similar to the SlpB of L. brevis ATCC 14869, was expressed. Slp-expressing SF9B cells exhibited increased survival in simulated gastrointestinal

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“…Indeed, the tolerance of bacteria towards digestive conditions is highly dependent on the strain used [12]. Among the considerations regarding the choice of bacterial strains, adherence to epithelial cells and mucus, as well as immunomodulatory properties are crucial to improve delivery efficiency of biologically active molecules or antigens [23].…”

Section: Editorialmentioning

confidence: 99%

“…Innovative probiotic delivery strategies should also take lessons from traditional fermented foods. Indeed, fermented milk and cheese [23,35,36] are highly versatile food products and may confer to dairy bacteria a level of stress tolerance that's hard to exceed. Indeed, they constitute a protective matrix rich in proteins and lipids allowing protection towards digestive enzymes.…”

Section: Editorialmentioning

confidence: 99%

Therapeutic DNA Vaccines: The Final Step for Success

Carmo¹,

Carvalho²

2017

J Bacteriol Parasitol

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The role of S-layer in adhesive and immunomodulating properties of probiotic starter culture Lactobacillus brevis D6 isolated from artisanal smoked fresh cheese

Cited by 39 publications

References 35 publications

Probiotic Propionibacterium freudenreichii requires SlpB protein to mitigate mucositis induced by chemotherapy

Probiotic Propionibacterium freudenreichii requires SlpB protein to mitigate mucositis induced by chemotherapy

Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut

Therapeutic DNA Vaccines: The Final Step for Success

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