Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut

Abstract

S-layers represent the simplest biological membranes developed during the evolution and are one of the most abundant biopolymers on Earth. Current fundamental and applied research aim to reveal the chemical structure, morphogenesis and function of S-layer proteins (Slps). This is the first paper that describes the Slps of certain Lactobacillus brevis strain isolated from sauerkraut. The whole genome sequence (WGS) analysis of the L. brevis SF9B strain uncovered three genes encoding the putative Slps, but merely one, identified as similar to the SlpB of L. brevis ATCC 14869, was expressed. Slp-expressing SF9B cells exhibited increased survival in simulated gastrointestinal

Colonisation potential of plantaricin-producing Lactobacillus plantarum SF9C andS-layer-carrying Lactobacillus brevis SF9B among gut microbiota

“…Genomic DNA was prepared according to Frece et al (2009). Genome sequencing was done using a paired-end approach as essentially described in Banić et al (2018). Briefly, the Nextera DNA Library Preparation Kit (Illumina, San Diego, CA, USA) was used to construct a library.…”

“…Slps have a functional role in conveying increased survival of the respective strain in simulated GIT conditions and during freeze-drying. Moreover, the results indicate a prominent role of Slps in adhesion of SF9B strain to mucin, extracellular matrix (ECM) proteins, and particularly to Caco-2 cells (Banić et al, 2018). Besides SF9B, an autochthonous isolate SF9C was isolated at the final stage of spontaneous fermentation.…”

“…Previously, we monitored lactic acid bacteria (LAB) population during spontaneous fermentation of the Brassica oleracea var. capitata cultivar Varaždinski (Banić et al, 2018;Beganović et al, 2014;Beganović et al, 2011). At the onset of the spontaneous fermentation, LAB diversity was present, including Leuconostoc mesenteroides strains, while a restricted number of Lactobacillus species, mainly Lactobacillus plantarum, dominated in the latter stages (Beganović et al, 2014).…”

“…brevis SF9B is a non-producing bacteriocin strain, but whose genome contains plnI gene, encoding the bacteriocin immunity protein(Banić et al, 2018). Both strains, SF9B and SF9C have shown tolerance to harsh conditions of GIT because their relative survival rate displayed only a 2-log and 3-log decrease in CFU/ml, respectively, under the conditions mimicking the GIT(Banić et al, 2018;Beganović et al, 2014). The cooperation of coexisting…”

Colonization potential of plantaricin-producing Lactobacillus plantarum SF9C and S-layer carrying Lactobacillus brevis SF9B among gut microbiota

“…Previously, we monitored lactic acid bacteria (LAB) population during spontaneous fermentation of the Brassica oleracea var. capitata cultivar Varaždinski (Banić et al, 2018;Beganović et al, 2014;Beganović et al, 2011a). At the onset of the spontaneous fermentation, a LAB diversity is present, including Leuconostoc mesenteroides strains, while a restricted number of Lactobacillus species dominates in the latter stages, consisting mainly of Lactobacillus plantarum (Beganović et al, 2014).…”