The role of regulatory RNAs (miRNAs) in asthma

Svitich, Oxana; Соболев, В. В.; Gankovskaya, L. V.; Zhigalkina, P.V.; Zverev, Vitaly

doi:10.1016/j.aller.2017.09.015

Cited by 30 publications

(16 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…MiRNAs play important roles in both normal and pathological processes [19]. Many studies have found that plasma/serum miRNAs are promising noninvasive biomarkers aiding diagnosis; circulating miRNAs are very stable and are easily assayed [16], [20].…”

Section: Discussionmentioning

confidence: 99%

The Serum microRNA Profile of Intrahepatic Cholestasis of Pregnancy: Identification of Novel Noninvasive Biomarkers

Zou¹,

Luo²,

Zhao³

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific disease that significantly increases the risk of fetal complications. Here, we measured serum miRNA levels in ICP patients to identify candidate biomarkers for ICP. Methods: We used the Agilent miRNA array followed by reverse transcription-polymerase chain reaction assays to identify and validate the serum miRNA profiles of 40 pregnant women with ICP and 40 healthy pregnant controls. We used bioinformatics to identify metabolic processes related to differentially expressed miRNAs. Results: The expression levels of three miRNAs (miR-371a- 5p, miR-6865-5p, and miR-1182) were significantly increased in ICP patients compared to controls; the areas under the receiver operating characteristic (ROC) curves (AUCs) were 0.771, 0.811, and 0.798, respectively. Multiple logistic regression analysis showed that a combination of the levels of the three miRNAs afforded a greater AUC (0.845), thus more reliably diagnosing ICP. The levels of all three miRNAs were positively associated with that of total bile acids. Furthermore, bioinformatics analysis indicated that the three miRNAs principally affected lipid phosphorylation, apoptosis, and the MAPK signaling pathway. Conclusion: This preliminary work improves our understanding of serum miRNA changes in pregnant women with ICP. The three miRNAs may serve as novel noninvasive biomarkers of ICP.

show abstract

Section: Discussionmentioning

confidence: 99%

The Serum microRNA Profile of Intrahepatic Cholestasis of Pregnancy: Identification of Novel Noninvasive Biomarkers

Zou¹,

Luo²,

Zhao³

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Exploring non-coding variation has been also proposed as a promising strategy to disentangle the genetic basis of complex diseases (114) such as, microRNAs (miRNAs). These are short, non-coding and single-strand RNA molecules that interact with different genomic elements and regulate gene expression at transcriptional level (148, 184, 194). Interestingly, these are involved in the regulation of the stability of immune cells and the intensity of inflammation (194).…”

Section: Asthma Prediction and Translation Into The Clinicmentioning

confidence: 99%

“…These are short, non-coding and single-strand RNA molecules that interact with different genomic elements and regulate gene expression at transcriptional level (148, 184, 194). Interestingly, these are involved in the regulation of the stability of immune cells and the intensity of inflammation (194). In fact, miRNAs have been proposed as potential non-invasive asthma biomarkers that could be used for asthma diagnosis (195, 196).…”

Section: Asthma Prediction and Translation Into The Clinicmentioning

confidence: 99%

Genomic Predictors of Asthma Phenotypes and Treatment Response

2019

View full text Add to dashboard Cite

Asthma is a complex respiratory disease considered as the most common chronic condition in children. A large genetic contribution to asthma susceptibility is predicted by the clustering of asthma and allergy symptoms among relatives and the large disease heritability estimated from twin studies, ranging from 55 to 90%. Genetic basis of asthma has been extensively investigated in the past 40 years using linkage analysis and candidate-gene association studies. However, the development of dense arrays for polymorphism genotyping has enabled the transition toward genome-wide association studies (GWAS), which have led the discovery of several unanticipated asthma genes in the last 11 years. Despite this, currently known risk variants identified using many thousand samples from distinct ethnicities only explain a small proportion of asthma heritability. This review examines the main findings of the last 2 years in genomic studies of asthma using GWAS and admixture mapping studies, as well as the direction of studies fostering integrative perspectives involving omics data. Additionally, we discuss the need for assessing the whole spectrum of genetic variation in association studies of asthma susceptibility, severity, and treatment response in order to further improve our knowledge of asthma genes and predictive biomarkers. Leveraging the individual's genetic information will allow a better understanding of asthma pathogenesis and will facilitate the transition toward a more precise diagnosis and treatment.

show abstract

“…Therefore, exploring and developing highly effective and individualized new drugs to treat asthma is of great significance. MiRNA agonists or antagonists hold the promise as biomarkers and targets of therapy in asthma [23, 24]. Inhibition of miRNA-145 by antagomir can reduce eosinophilic inflammation, mucus hypersecretion, Th2 cytokine production, and airway hyperresponsiveness, which is comparable to glucocorticoid treatment [25].…”

Section: Discussionmentioning

confidence: 99%

Divergent Roles of miR-3162-3p in Pulmonary Inflammation in Normal and Asthmatic Mice as well as Antagonism of miR-3162-3p in Asthma Treatment

Liu

Chen

Zhang

et al. 2020

Int Arch Allergy Immunol

View full text Add to dashboard Cite

MicroRNA (miRNA) mimics or antagomirs hold great promise for asthma treatment compared with glucocorticoids as mainstay therapy for asthma. But the role of miRNA in regulating asthmatic inflammation is largely unclear. We previously reported that miR-3162-3p in the peripheral blood of children with asthma was obviously upregulated compared to that in healthy children. This study aimed to elucidate the role of miR-3162-3p in pulmonary inflammation in normal and asthmatic mice as well as preliminarily explore the potential of miR-3162-3p antagomir in asthma treatment. A noninvasive whole-body plethysmograph measured airway responsiveness. Both qRT-PCR and Western blot were used to detect the expression of miRNA, mRNA, or protein. Cells in bronchoalveolar lavage fluid were counted by platelet counting and Wright's staining. Inflammatory infiltration and mucus secretion were identified by hematoxylin and eosin and periodic acid-Schiff staining, respectively. Cytokines in the lungs were detected by ELISA. The miR-3162-3p mimic intraperitoneally administered to normal mice decreased β-catenin levels in the lungs without obviously altering the lung histology and cytokine levels. Antagonizing miR-3162-3p in ovalbumin-induced asthmatic mice effec-Juman Liua, Yinhui Chena, and Feng Zhang contributed equally to this work. Edited by: H.-U. Simon, Bern.

show abstract

The role of regulatory RNAs (miRNAs) in asthma

Abstract: MicroRNAs play an important role in the regulation of many cellular processes. Undoubtedly, these regulatory molecules are involved in the pathogenesis of asthma, and therefore can be potential targets for treatment.

Cited by 30 publications

References 29 publications

The Serum microRNA Profile of Intrahepatic Cholestasis of Pregnancy: Identification of Novel Noninvasive Biomarkers

The Serum microRNA Profile of Intrahepatic Cholestasis of Pregnancy: Identification of Novel Noninvasive Biomarkers

Genomic Predictors of Asthma Phenotypes and Treatment Response

Divergent Roles of miR-3162-3p in Pulmonary Inflammation in Normal and Asthmatic Mice as well as Antagonism of miR-3162-3p in Asthma Treatment

Contact Info

Product

Resources

About