The role of putative intragenic control elements in c‐<i>fos</i> regulation by calcium and growth factor signalling pathways

Lange, Claudia M.; Bading, Hilmar

doi:10.1046/j.1471-4159.2001.00342.x

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…One report seems to rule out the contribution of intragenic sequences in calcium- and growth factor-induced c- fos transcription [35]. Since the latter study was based on transiently transfected constructs, these discrepancies could be due either to the different cell types used or to a requirement for chromatin structure that is not reproduced on extra-chromosomal templates.…”

Section: Discussionmentioning

confidence: 99%

A Novel Mouse c-fos Intronic Promoter That Responds to CREB and AP-1 Is Developmentally Regulated In Vivo

Coulon¹,

Chébli²,

Cavelier³

et al. 2010

PLoS ONE

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BackgroundThe c-fos proto-oncogene is an archetype for rapid and integrative transcriptional activation. Innumerable studies have focused on the canonical promoter, located upstream from the transcriptional start site. However, several regulatory sequences have been found in the first intron.Methodology/Principal FindingsHere we describe an extremely conserved region in c-fos first intron that contains a putative TATA box, and functional TRE and CRE sites. This fragment drives reporter gene activation in fibroblasts, which is enhanced by increasing intracellular calcium and cAMP and by cotransfection of CREB or c-Fos/c-Jun expression vectors. We produced transgenic mice expressing a lacZ reporter controlled by the intronic promoter. Lac Z expression of this promoter is restricted to the developing central nervous system (CNS) and the mesenchyme of developing mammary buds in embryos 12.5 days post-conception, and to brain tissue in adults. RT-QPCR analysis of tissue mRNA, including the anlage of the mammary gland and the CNS, confirms the existence of a novel, nested mRNA initiated in the first intron.Conclusions/SignificanceOur results provide evidence for a novel, developmentally regulated promoter in the first intron of the c-fos gene.

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Section: Discussionmentioning

confidence: 99%

A Novel Mouse c-fos Intronic Promoter That Responds to CREB and AP-1 Is Developmentally Regulated In Vivo

Coulon¹,

Chébli²,

Cavelier³

et al. 2010

PLoS ONE

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“…The second one was found only in tetrapoda. The third segment is conserved in all species and contains a cAMP response element in humans (Lange and Bading 2001). This division into three segments indicates that each segment has a different function.…”

Section: C-fos First Intronmentioning

confidence: 99%

Discovery of Regulatory Elements by a Computational Method for Phylogenetic Footprinting

Blanchette¹,

Tompa²

2002

Genome Res.

281

200

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Phylogenetic footprinting is a method for the discovery of regulatory elements in a set of orthologous regulatory regions from multiple species. It does so by identifying the best conserved motifs in those orthologous regions. We describe a computer algorithm designed specifically for this purpose, making use of the phylogenetic relationships among the sequences under study to make more accurate predictions. The program is guaranteed to report all sets of motifs with the lowest parsimony scores, calculated with respect to the phylogenetic tree relating the input species. We report the results of this algorithm on several data sets of interest. A large number of known functional binding sites are identified by our method, but we also find several highly conserved motifs for which no function is yet known.One of the great challenges currently facing biologists is to understand the varied and complex mechanisms that regulate gene expression. We focus on one important aspect of this challenge, the identification of binding sites for the factors involved in such regulation.A number of computer algorithms have been proposed for the discovery of novel regulatory elements in nucleotide sequences. Most of these try to deduce the regulatory elements by considering the regulatory regions of several (putatively) coregulated genes from a single genome. Such algorithms search for overrepresented motifs in this collection of regulatory regions, these motifs being good candidates for regulatory elements. Examples of this approach include van

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The early signaling pathway of live yeast cell derivative in THP-1 monocytes

et al. 2018

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The role of putative intragenic control elements in c‐fos regulation by calcium and growth factor signalling pathways

Cited by 3 publications

References 33 publications

A Novel Mouse c-fos Intronic Promoter That Responds to CREB and AP-1 Is Developmentally Regulated In Vivo

A Novel Mouse c-fos Intronic Promoter That Responds to CREB and AP-1 Is Developmentally Regulated In Vivo

Discovery of Regulatory Elements by a Computational Method for Phylogenetic Footprinting

The early signaling pathway of live yeast cell derivative in THP-1 monocytes

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