The role of promoter methylation in the regulation of genes encoding succinate dehydrogenase in maize seedlings

Abstract

The activity of succinate dehydrogenase (SDH) changed during maize (Zea mays L.) seed germi nation. On the fifth day of germination, the activity was the highest, which might be evidently explained by accelerated utilization of stored carbohydrates and lipids. Subsequent decrease in SDH activity might be related to metabolism switching to autotrophic nutrition. The correlation was found between the levels of sdh1 1 and sdh1 2 gene transcription and the extent of methylation of their promoters. During seed germi nation, promoter methylation of genes encoding the subunit A of SDH was activated, which resulted in sup pression of their transcription. It is suggested that this mechanism may play an important role in the regula tion of sdh1 1 and sdh1 2 gene transcription in plant tissues.

Single-base resolution methylomes of upland cotton (Gossypium hirsutum L.) reveal epigenome modifications in response to drought stress

“…In each gene element, the frequencies of each context, including CG methylation, CHG methylation and CHH methylation, were disparate, and CG methylation accounted for approximately half of the total m C, which suggested that CG methylation was the most important methylation pattern in the three gene elements. Previous publications have showed that methylation most frequently occurs in the so-called CpG islands in the 5’ regulatory gene regions (promoters) [ 26 ]. We then compared the methylation levels of different contexts in promoter, exon and intron pre- and post-drought stress, and minuscule changes were found, except for CHH methylation (Fig.…”

Whole genome bisulfite sequencing methylome analysis of mulberry (Morus alba) reveals epigenome modifications in response to drought stress

“…Repeat sequences were the most highly methylated among genomic regions assessed. In maize seedlings, methylation most frequently occurred in CpG islands within the 5′ regulatory regions of genes (promoters) 48 . To reveal the relationship between DNA methylation profiles and genes expression levels, canonical DNA methylation profiles of the entire transcriptional units were also divided into seven distinct functional elements in castor bean seeds 49,50 .…”

Phytochrome‐mediated regulation of plant respiration and photorespiration

“…Regulation of expression of SDH genes also involves methylation of their promoters (Eprintsev et al . ).…”

“…This suggests that SDH can be regulated at the expression level by the phytochrome system with the involvement of calcium ions as signal transducing messengers. Regulation of expression of SDH genes also involves methylation of their promoters (Eprintsev et al 2012).…”