1967
DOI: 10.1021/bi00864a014
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The Role of Primer in Glycogen Biosynthesis in Aerobacter aerogenes*

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Cited by 25 publications
(18 citation statements)
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“…The same ratio of components was maintained in reaction mixtures of larger volume. Reaction mixtures were incubated at 37 C, and the radioactivity incorporated into alcoholinsoluble polysaccharides was determined by spotting samples (50 Mliters) on filter paper squares, washing the papers in 66% ethanol, and measuring the retained radioactivity as described elsewhere (10,18). A unit of dextransucrase or levansucrase is arbitrarily defined as that amount of enzyme that incorporates 1 smol of glucose or fructose, respectively, from sucrose into polysaccharide per min at pH 6.8 and 37 C. Dextransucrase activities in Table 1 were determined from the amount of fructose released in the assay digests.…”
mentioning
confidence: 99%
“…The same ratio of components was maintained in reaction mixtures of larger volume. Reaction mixtures were incubated at 37 C, and the radioactivity incorporated into alcoholinsoluble polysaccharides was determined by spotting samples (50 Mliters) on filter paper squares, washing the papers in 66% ethanol, and measuring the retained radioactivity as described elsewhere (10,18). A unit of dextransucrase or levansucrase is arbitrarily defined as that amount of enzyme that incorporates 1 smol of glucose or fructose, respectively, from sucrose into polysaccharide per min at pH 6.8 and 37 C. Dextransucrase activities in Table 1 were determined from the amount of fructose released in the assay digests.…”
mentioning
confidence: 99%
“…U -14 C-Sucrose was purchased from New England Nuclear. The incorporation of U-14C-glucose into the total dextran was measured by placing aliquots of 14 C-sucrose-dextransucrase digests on squares of filter paper, and washing the paper with 66% ethanol and then acetone to remove all low molecular weight radioactive molecules, as by Kindt and Conrad [6]. The papers were dried and counted in scintillation solution containing 5 g diphenyloxazole and 0.5 g p-bis [2-(5-phenyloxazolyl)]-benzene per liter of toluene, using aPackard Tri-carb scintillation spectrometer.…”
mentioning
confidence: 99%
“…Paper chromatograms were run in 1-butanol-pyridine-water (6:4:3) and standards were detected by spraying with aniline acid phthalate reagent (Partridge, 1949). Radioactive compounds on chromatograms were quantitated by cutting the chromatograms into segments and counting the segments as before (Kindt and Conrad, 1967).…”
Section: Methodsmentioning
confidence: 99%
“…The diversity of physical and chemical properties of the compounds which stimulate glycogen synthetase suggests that they are all activators of glycogen synthetase rather than acceptors for the glucosyl resic V_J"lycogen synthetases, regardless of their source, have shown an absolute requirement for a glycogentype glucan to accept glucose from the system-specific nucleoside diphosphate glucose (Leloir and Cardini, 1962;Greenberg and Preiss, 1965;Preiss and Greenberg, 1965). In previous work (Kindt and Conrad, 1967) it was shown that extracts of late log cells of Aerobacter aerogenes A3(S1) would synthesize glycogen from ADP-glucose only when exogenous glycogen was added, in spite of the fact that up to 2 g of glycogen/100 g of dry cells could be extracted from stationary-phase cultures of this organism. The tentative conclusion based on these observations was that late-log cells, in contrast to stationary-phase cells, were devoid of glycogen.…”
mentioning
confidence: 99%