The Role of PPK26 in Drosophila Larval Mechanical Nociception

Guo, Yanmeng; Wang, Yuping; Wang, Qingxiu; Wang, Zuoren

doi:10.1016/j.celrep.2014.10.020

Cited by 81 publications

(80 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4A-D). Moreover, in class IV neurons, we found that the DEG/ ENaC channels ppk1 and ppk26, which are necessary for the function of the dendrites in mechanical nociception (Zhong et al 2010;Gorczyca et al 2014;Guo et al 2014;Mauthner et al 2014), are properly trafficked into regenerated dendrites and properly excluded from the axon (Fig. 6B,C).…”

Section: Dendrite Balding Does Not Alter Dendritic Characteristics Ormentioning

confidence: 96%

“…The DEG/ENaC channels ppk1 and ppk26, which are necessary for mechanical nociception, are normally specifically expressed by class IV da neurons (Zhong et al 2010;Gorczyca et al 2014;Guo et al 2014;Mauthner et al 2014). We showed above that these proteins are properly trafficked into regenerated dendrites and excluded from axons, but are they still expressed by the correct neuronal subtype?…”

Section: Dendrite Balding Does Not Alter Dendritic Characteristics Ormentioning

confidence: 99%

See 1 more Smart Citation

In vivo dendrite regeneration after injury is different from dendrite development

Thompson-Peer

DeVault

et al. 2016

Genes Dev.

View full text Add to dashboard Cite

Neurons receive information along dendrites and send signals along axons to synaptic contacts. The factors that control axon regeneration have been examined in many systems, but dendrite regeneration has been largely unexplored. Here we report that, in intact Drosophila larvae, a discrete injury that removes all dendrites induces robust dendritic growth that recreates many features of uninjured dendrites, including the number of dendrite branches that regenerate and responsiveness to sensory stimuli. However, the growth and patterning of injury-induced dendrites is significantly different from uninjured dendrites. We found that regenerated arbors cover much less territory than uninjured neurons, fail to avoid crossing over other branches from the same neuron, respond less strongly to mechanical stimuli, and are pruned precociously. Finally, silencing the electrical activity of the neurons specifically blocks injury-induced, but not developmental, dendrite growth. By elucidating the essential features of dendrites grown in response to acute injury, our work builds a framework for exploring dendrite regeneration in physiological and pathological conditions.

show abstract

Section: Dendrite Balding Does Not Alter Dendritic Characteristics Ormentioning

confidence: 96%

Section: Dendrite Balding Does Not Alter Dendritic Characteristics Ormentioning

confidence: 99%

In vivo dendrite regeneration after injury is different from dendrite development

Thompson-Peer

DeVault

et al. 2016

Genes Dev.

View full text Add to dashboard Cite

show abstract

“…The TRPN channel NOMPC functions in class III da neurons to mediate gentle touch sensation (13) whereas the DEG/ENaC ion channels PPK and PPK26, the TRP channel Painless, and Piezo function in class IV da neurons to mediate mechanical nociception (10,20,(46)(47)(48). As to proprioception, chordotonal organs, class I and class IV da neurons and bd neurons may all contribute to proprioception to regulate larval locomotion behavior (5)(6)(7)(8)10).…”

Section: Differential Functions Of Different Sensory Neurons In Regulmentioning

confidence: 99%

“…The 5′ and 3′ homologous arms of tmc were amplified from w 1118 flies by PCR cloning and cloned into pw35-Gal4 vectors using the pEASY-Uni Seamless Cloning and Assembly Kit (Beijing TransGen Biotech Co.). Mutagenesis was performed as previously described (37,48). Larval neuron staining.…”

Section: Mutagenesis the Tmcmentioning

confidence: 99%

Transmembrane channel-like ( tmc ) gene regulates Drosophila larval locomotion

Guo¹,

Wang²,

Zhang³

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Drosophila larval locomotion, which entails rhythmic body contractions, is controlled by sensory feedback from proprioceptors. The molecular mechanisms mediating this feedback are little understood. By using genetic knock-in and immunostaining, we found that the Drosophila melanogaster transmembrane channel-like (tmc) gene is expressed in the larval class I and class II dendritic arborization (da) neurons and bipolar dendrite (bd) neurons, both of which are known to provide sensory feedback for larval locomotion. Larvae with knockdown or loss of tmc function displayed reduced crawling speeds, increased head cast frequencies, and enhanced backward locomotion. Expressing Drosophila TMC or mammalian TMC1 and/or TMC2 in the tmc-positive neurons rescued these mutant phenotypes. Bending of the larval body activated the tmc-positive neurons, and in tmc mutants this bending response was impaired. This implicates TMC's roles in Drosophila proprioception and the sensory control of larval locomotion. It also provides evidence for a functional conservation between Drosophila and mammalian TMCs.proprioception | locomotion | mechanosensation

show abstract

“…Liu et al, 2003aLiu et al, ,b, 2012Lu et al, 2012;Starostina et al, 2012;Thistle et al, 2012;Toda et al, 2012;Zelle et al, 2013;Guo et al, 2014;Mast et al, 2014;Vijayan et al, 2014 Larval terminal organ…”

Section: Olfactory and Gustatory Chemoreceptors In Flies: Several Recmentioning

confidence: 99%

Chemicals and chemoreceptors: ecologically relevant signals driving behavior in Drosophila

2015

View full text Add to dashboard Cite

Insects encounter a vast repertoire of chemicals in their natural environment, which can signal positive stimuli like the presence of a food source, a potential mate, or a suitable oviposition site as well as negative stimuli such as competitors, predators, or toxic substances reflecting danger. The presence of specialized chemoreceptors like taste and olfactory receptors allows animals to detect chemicals at short and long distances and accordingly, trigger proper behaviors toward these stimuli. Since the first description of olfactory and taste receptors in Drosophila melanogaster 15 years ago, our knowledge on the identity, properties, and function of specific chemoreceptors has increased exponentially. In the last years, multidisciplinary approaches combining genetic tools with electrophysiological techniques, behavioral recording, evolutionary analysis, and chemical ecology studies are shedding light on our understanding on the ecological relevance of specific chemoreceptors for the survival of Drosophila in their natural environment. In this review we discuss the current knowledge on chemoreceptors of both the olfactory and taste systems of the fruitfly. We focus on the relevance of particular receptors for the detection of ecologically relevant cues such as pheromones, food sources, and toxic compounds, and we comment on the behavioral changes that the detection of these chemicals induce in the fly. In particular, we give an updated outlook of the chemical communication displayed during one of the most important behaviors for fly survival, the courtship behavior. Finally, the ecological relevance of specific chemicals can vary depending on the niche occupied by the individual. In that regard, in this review we also highlight the contrast between adult and larval systems and we propose that these differences could reflect distinctive requirements depending on the change of ecological niche occupied by Drosophila along its life cycle.

show abstract

The Role of PPK26 in Drosophila Larval Mechanical Nociception

Cited by 81 publications

References 39 publications

In vivo dendrite regeneration after injury is different from dendrite development

In vivo dendrite regeneration after injury is different from dendrite development

Transmembrane channel-like ( tmc ) gene regulates Drosophila larval locomotion

Chemicals and chemoreceptors: ecologically relevant signals driving behavior in Drosophila

Contact Info

Product

Resources

About