The role of pleiotrophin in bone repair

Lamprou, Margarita; Kaspiris, Angelos; Panagiotopoulos, Elias; Giannoudis, Peter V.; Papadimitriou, Evangelia

doi:10.1016/j.injury.2014.10.013

Cited by 45 publications

(33 citation statements)

References 100 publications

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“…Heparin-binding proteins are present in most living organisms and have a role in a variety of physiological functions including angiogenesis (pleiotrophin 23 ) cell proliferation and migration (growth factors 24 ), coagulation (antithrombin 25 ), male fertility 26 and innate immunity (cathelicidin, chemokines 27 ; cathepsin G, elastase 28 ). The heparin-binding domain is characterized by a stretch of positively charged amino-acids and would act similarly to cationic antimicrobial peptides, by interacting with the negatively charged bacterial surface such as lipopolysaccharide or lipoteichoic acid, which leads to the disruption of the bacterial plasma membrane 4 5 29 .…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of egg white heparin-binding proteins: towards the identification of natural antibacterial molecules

Guyot¹,

Labas

Harichaux

et al. 2016

Sci Rep

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The chicken egg resists most environmental microbes suggesting that it potentially contains efficient antimicrobial molecules. Considering that some heparin-binding proteins in mammals are antibacterial, we investigated the presence and the antimicrobial activity of heparin-binding proteins from chicken egg white. Mass spectrometry analysis of the proteins recovered after heparin-affinity chromatography, revealed 20 proteins, including known antimicrobial proteins (avidin, lysozyme, TENP, ovalbumin-related protein X and avian bêta-defensin 11). The antibacterial activity of three new egg candidates (vitelline membrane outer layer protein 1, beta-microseminoprotein-like (LOC101750704) and pleiotrophin) was demonstrated against Listeria monocytogenes and/or Salmonella enterica Enteritidis. We showed that all these molecules share the property to inhibit bacterial growth through their heparin-binding domains. However, vitelline membrane outer layer 1 has additional specific structural features that can contribute to its antimicrobial potential. Moreover, we identified potential supplementary effectors of innate immunity including mucin 5B, E-selectin ligand 1, whey acidic protein 3, peptidyl prolyl isomerase B and retinoic acid receptor responder protein 2. These data support the concept of using heparin affinity combined to mass spectrometry to obtain an overview of the various effectors of innate immunity composing biological milieus, and to identify novel antimicrobial candidates of interest in the race for alternatives to antibiotics.

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Section: Discussionmentioning

confidence: 99%

Proteomic analysis of egg white heparin-binding proteins: towards the identification of natural antibacterial molecules

Guyot¹,

Labas

Harichaux

et al. 2016

Sci Rep

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“…During mouse odontogenesis, PTN expression was detected in ameloblasts, odontoblasts, dental follicle, and PDL 39 . In the mouse, targeted PTN overexpression in the bone inhibited BMP2 ‐mediated bone induction, impairing fracture healing 38 . Conversely, Ptn ablation in mice is associated with improvement in hematopoetic stem cell function: loss of microenvironmental Ptn leads to enhancement of stem cell maintenance in vivo and stem cell repopulation ability in vitro 40 …”

Section: Discussionmentioning

confidence: 99%

“…PTN is a secreted angiogenic GF, exerting a pleotropic effect by modulating various processes, such bone remodeling, angiogenesis, fracture healing, and osteoarthritis. 38 During mouse odontogenesis, PTN expression was detected in ameloblasts, odontoblasts, dental follicle, and PDL. 39 In the mouse, targeted PTN overexpression in the bone inhibited BMP2-mediated bone induction, impairing fracture healing.…”

Section: Discussionmentioning

confidence: 99%

Aspirin Enhances Osteogenic Potential of Periodontal Ligament Stem Cells (PDLSCs) and Modulates the Expression Profile of Growth Factor–Associated Genes in PDLSCs

Rahman

Ali

Abdullah

et al. 2016

Journal of Periodontology

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ASA modulates the expression of growth factor-associated genes and enhances osteogenic potential in PDLSCs. ASA upregulated the expression of genes that could activate biologic functions and canonic pathways related to cell proliferation, human embryonic stem cell pluripotency, tissue regeneration, and differentiation. These findings suggest that ASA enhances PDLSC function and may be useful in regenerative dentistry applications, particularly in the areas of periodontal health and regeneration.

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“…The interaction between the multifunctional growth factor midkine and heparin is unusual in that the hinge region between the two domains is important for heparin binding, as determined by an NMR study using the pentasaccharide fondaparinux (Lim et al, 2013). Pleiotrophin is associated with chondroitin sulfate-mediated neurite outgrowth and is important in bone repair (Lamprou et al, 2014).…”

Section: Heparin and Cytokines Growth Factors Selectins And Promentioning

confidence: 99%