Aspirin Enhances Osteogenic Potential of Periodontal Ligament Stem Cells (PDLSCs) and Modulates the Expression Profile of Growth Factor–Associated Genes in PDLSCs

Rahman, Fazliny Abd; Ali, Johari Mohd; Abdullah, Maimon; Kasim, Noor Hayaty Abu; Musa, Sabri

doi:10.1902/jop.2016.150610

Cited by 36 publications

(27 citation statements)

References 47 publications

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“…[40][41][42] Therefore, in order to investigate whether the Wnt/β-catenin signalling pathway might be the potential mechanism through which TDNs facilitate PDLSCs osteogenic differentiation, we performed western blotting (WB) and immunofluorescence staining in order to analyse the protein expression of LEF-1, GSK-3β and β-catenin. But some previous studies have demonstrated that the Wnt/β-catenin signalling pathway plays a significant role in the osteogenic differentiation of PDLSCs.…”

Section: Mechanism Through Which Tdn Regulated the Osteogenic Diffementioning

confidence: 99%

Effect of tetrahedral DNA nanostructures on proliferation and osteogenic differentiation of human periodontal ligament stem cells

et al. 2019

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Objective To explore the effects and underlying biological mechanisms of tetrahedral DNA nanostructures (TDNs) on the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Materials and methods Real‐time cell analysis (RTCA) and CCK8 were used to screen the best concentration of TDN for PDLSCs. Cell proliferation and osteogenic differentiation were assessed after PDLSCs were treated with TDN. Data were analysed using one‐way ANOVA. Results Tetrahedral DNA nanostructures could play a crucial role in accelerating the proliferation of PDLSCs and had the strongest promotive effect on PDLSCs at a concentration of 250 nmol/L. Simultaneously, the osteogenic differentiation of PDLSCs could be promoted significantly by TDNs and the finding displayed that the Wnt/β‐catenin signalling pathway might be the underlying biological mechanisms of TDNs on promoting the osteogenic differentiation of PDLSCs. Conclusion Tetrahedral DNA nanostructure treatment facilitated the proliferation of PDLSCs, significantly promoted osteogenic differentiation by regulating the Wnt/β‐catenin signalling pathway. Therefore, TDNs could be a novel nanomaterial with great potential for application to PDLSC‐based bone tissue engineering.

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Section: Mechanism Through Which Tdn Regulated the Osteogenic Diffementioning

confidence: 99%

Effect of tetrahedral DNA nanostructures on proliferation and osteogenic differentiation of human periodontal ligament stem cells

et al. 2019

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“…hPDLSCs can differentiate into both bone-like tissue and tissue similar to the natural periodontal membrane connective tissue. PDLSCs are ideal seed cells in periodontal tissue regeneration [2–4]. Thus, an increasing number of studies on PDLSCs have been carried out in recent years, requiring the production of more hPDLSCs.…”

Section: Introductionmentioning

confidence: 99%

Effect of YAP on an Immortalized Periodontal Ligament Stem Cell Line

Chen

et al. 2019

Stem Cells International

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Objective. To establish an immortalized human periodontal ligament stem cell line (hPDLSC) and investigate whether and how YAP mediates the establishment of the stem cell line. Methods. Primary hPDLSCs were cultured and transfected with lentivirus containing the telomerase reverse transcriptase (TERT) gene. The expression of TERT was detected via the polymerase chain reaction (PCR) and real-time quantitative PCR (RT-PCR). Flow cytometry was employed to detect surface markers of hPDLSCs and TERT-hPDLSCs. The cell counting kit-8 (CCK-8) and 5-ethynyl-2’-deoxyuridine (EdU) methods were used to examine the proliferation ability of the cells. Flow cytometry and TUNEL staining were employed to examine the cell apoptosis rate. The β-galactosidase staining assay was used to assess the rate of cell senescence. The osteogenic differentiation ability of the cells was detected via alkaline phosphatase (ALP) staining and Alizarin red staining assays. BALB/c mice were employed to determine the tumorigenicity of TERT-hPDLSCs. The expression levels of YAP and other proteins in the Hippo signaling pathway were detected by Western blotting. Verteporfin was used to inhibit the binding of YAP to the downstream target gene TEAD. Results. TERT-hPDLSCs showed stable high expression of TERT, even at the thirtieth passage after transfection with lentivirus containing the TERT gene. Compared with primary hPDLSCs, TERT-hPDLSCs exhibited a stronger proliferation ability and lower cell apoptosis and senescence rates while maintaining the same osteogenetic differentiation ability as primary hPDLSCs. The transfection of hPDLSCs with lentivirus containing the TERT gene did not lead to tumorigenesis in nude mice. The Hippo signaling pathway was inactivated in TERT-hPDLSCs compared to hPDLSCs. When treated with verteporfin, the proliferation of TERT-hPDLSCs decreased, while the apoptosis and senescence rates of these cells increased. However, TERT-hPDLSCs still showed a stronger proliferation ability and lower cell apoptosis and senescence rates than hPDLSCs treated with verteporfin at the same concentration. Conclusions. Overexpression of TERT in hPDLSCs resulted in the successful establishment of an immortalized periodontal ligament stem cell line. TERT may regulate the biological characteristics of hPDLSCs through the Hippo/YAP signaling pathway. hPDLSCs could be a feasible resource for stem cell research and a promising resource for stem cell therapy.

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“…Administration of aspirin was capable of improving BMSC-mediated calvarial bone regeneration in a porcine model ( 10 ), as well as osteogenic differentiation and immunomodulation mediated by stem cells derived from exfoliated deciduous teeth ( 11 ). Aspirin has also been demonstrated to enhance the function of periodontal ligament stem cells and may have regenerative dentistry applications ( 12 ). Combined use of aspirin and adipose-derived stem cells has been reported to partially reverse bone loss caused by castration in rats ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

Aspirin promotes osteogenic differentiation of human dental pulp stem cells

Yuan

Zhan

et al. 2018

Int J Mol Med

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Human dental pulp stem cells (hDPSCs) possess self-renewal and osteogenic differentiation properties, and have been used for orofacial bone regeneration and periodontal treatment. Aspirin has been demonstrated to enhance the regeneration of bone marrow mesenchymal stem cells (MSCs); however, the impact of aspirin on the osteogenic differentiation of hDPSCs remains unknown. In the present study, hDPSCs were characterized by flow cytometry, while their clonogenic potential and multipotency were assessed using alizarin red, Oil red O and alcian blue staining. The effect of aspirin on hDPSC viability was assessed using Cell Counting Kit-8 assay. Osteogenic capacity was examined by alkaline phosphatase activity, alizarin red staining, reverse transcription-polymerase chain reaction and western blotting. Furthermore, in vivo cranial defects were established in Sprague-Dawley rats to evaluate the effect of aspirin on hDPSC-based bone regeneration. Anorganic bovine bone was used as a bone replacement material and as the carrier for hDPSCs. New bone formation was observed through radiographic and histological analysis. The study demonstrated that hDPSCs expressed MSC markers and possessed multipotency in vitro. Aspirin was non-toxic to hDPSCs at a concentration of ≤100 μg/ml and enhanced the osteogenesis of hDPSCs in vitro. Aspirin significantly increased hDPSC-based bone formation in the rat cranial defect model at 8 or 12 weeks post-implantation (P<0.05). The data suggested that aspirin promotes the osteogenic potential of hDPSCs in vitro and in vivo. Overall, the present study indicated that aspirin improves the bone regeneration capacity of hDPSCs.

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Aspirin Enhances Osteogenic Potential of Periodontal Ligament Stem Cells (PDLSCs) and Modulates the Expression Profile of Growth Factor–Associated Genes in PDLSCs

Cited by 36 publications

References 47 publications

Effect of tetrahedral DNA nanostructures on proliferation and osteogenic differentiation of human periodontal ligament stem cells

Effect of tetrahedral DNA nanostructures on proliferation and osteogenic differentiation of human periodontal ligament stem cells

Effect of YAP on an Immortalized Periodontal Ligament Stem Cell Line

Aspirin promotes osteogenic differentiation of human dental pulp stem cells

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