Effect of tetrahedral DNA nanostructures on proliferation and osteogenic differentiation of human periodontal ligament stem cells

Abstract

Objective To explore the effects and underlying biological mechanisms of tetrahedral DNA nanostructures (TDNs) on the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Materials and methods Real‐time cell analysis (RTCA) and CCK8 were used to screen the best concentration of TDN for PDLSCs. Cell proliferation and osteogenic differentiation were assessed after PDLSCs were treated with TDN. Data were analysed using one‐way ANOVA. Results Tetrahedral DNA nanostructures could play a crucial role in accelerating the proliferation of PDLSCs and had the strongest promotive effect on PDLSCs at a concentration of 250 nmol/L. Simultaneously, the osteogenic differentiation of PDLSCs could be promoted significantly by TDNs and the finding displayed that the Wnt/β‐catenin signalling pathway might be the underlying biological mechanisms of TDNs on promoting the osteogenic differentiation of PDLSCs. Conclusion Tetrahedral DNA nanostructure treatment facilitated the proliferation of PDLSCs, significantly promoted osteogenic differentiation by regulating the Wnt/β‐catenin signalling pathway. Therefore, TDNs could be a novel nanomaterial with great potential for application to PDLSC‐based bone tissue engineering.

Tetrahedral Framework Nucleic Acid Promotes the Treatment of Bisphosphonate-Related Osteonecrosis of the Jaws by Promoting Angiogenesis and M2 Polarization

“…Previous studies have shown that tFNAs have varying degrees of promoting effects on a variety of cells. A previous study has shown that tFNAs can promote osteogenic differentiation of human periodontal ligament stem cells . Therefore, in this study, in the in vivo experimental part, the process of tFNAs to promote jaw healing may be partly by directly regulating osteoblasts and osteoclasts.…”

“…A previous study has shown that tFNAs can promote osteogenic differentiation of human periodontal ligament stem cells. 63 Therefore, in this study, in the in vivo experimental part, the process of tFNAs to promote jaw healing may be partly by directly regulating osteoblasts and osteoclasts. This question is worthy of further study in our next experiment.…”

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

“…The effects of HA on osteogenesis are assessed by a number of methods. The most basic approaches measure the cell proliferation, which can be achieved through different techniques, from cell counting to double-stranded DNA (dsDNA) quantification, mitochondrial activity assessment, and real-time cell analysis [158]. These methods overlap with those used for assessing cytocompatibility/cytotoxicity and are covered more extensively in Section 2.2.…”

Advancements in nucleic acids‐based techniques for bone regeneration

“…After exposure to TDNs, the gene and protein expression analyses showed a significant increase in Runx2, OPN, ALP expression, while the cellular level assay increased in calcium nodule formation. [106,107] DNA nanostructures were used to increase the sensitivity of nontransformed mouse mammary gland epithelial cell (NMuMG) cells toward TGF-β ligand. The nanostructures were coupled with TGF-β via streptavidin, which enabled the complex to have multiple ligands.…”