Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

Albulescu, Radu; Popa, Ana Maria; Enciu, Ana‐Maria; Albulescu, Lucian; Dudău, Maria; Popescu, Ionela Daniela; Mihai, Simona; Codrici, Elena; Pop, Sevinci; Lupu, Andreea-Roxana; Stan, George E.; Manda, Gina; Tănase, Cristiana

doi:10.3390/ma12223704

Cited by 39 publications

(26 citation statements)

References 280 publications

(343 reference statements)

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“…Generally, antibacterial ability conflicts with biocompatibility. Therefore, to evaluate the biocompatibility of protamine/HAp, in vitro cytotoxicity tests were carried out according to ISO 10993-5:2009 “Tests for Cytotoxicity—In vitro Methods” [ 20 ]. Human osteoblastic cells (MG-63) were cultured in extracts from various kinds of powders (0.1 mg·mL −1 ) for 24 and 72 h. As shown in Figure 7 , the results of the MTT assay at 24 and 72 h showed that no significant changes of cell viability were seen in concentrations ranging from 125 to 500 μg·mL −1 .…”

Section: Resultsmentioning

confidence: 99%

“…To evaluate the antibacterial activity, Escherichia coli (E. coli, ATCC 27325) and Staphylococcus aureus (S. aureus, ATCC 6538) were used in this study. Bacteria were grown in a culture broth (nutrient USA) supplemented with 10% heat-inactivated fetal bovine serum (Sigma, St. Louis, MO, USA) and 0.1% antibiotics (penicillin-streptomycin solution, Sigma) in a humidified atmosphere containing 5% CO 2 at 37 • C. According to ISO 10993-5:2009 "Tests for Cytotoxicity-In vitro Methods" [20], extracts were obtained in a separate culture medium (0.1 g•mL −1 of the culture medium for 24 h at 37 • C). For cytotoxicity test, cells were seeded at 5 × 10 3 cells•well -1 on 96-well plates and cultured for 24 h at 37 • C/5% CO 2 .…”

Section: Antimicrobial Evaluationmentioning

confidence: 99%

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Potential Application of Protamine for Antimicrobial Biomaterials in Bone Tissue Engineering

Honda

Matsumoto

Aizawa

2020

IJMS

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Bacterial infection of biomaterials is a serious problem in the field of medical devices. It is urgently necessary to develop new biomaterials with bactericidal activity. Antimicrobial peptides and proteins (AMPs), alternative antibacterial agents, are expected to overcome the bacterial resistance. The aim of this study was to develop a new intelligent material in bone tissue engineering based on protamine-loaded hydroxyapatite (protamine/HAp) that uses AMPs rather than antibiotics. It was found that the adsorption of protamine to HAp followed the Langmuir adsorption model and was due to electrostatic and/or hydrophobic interactions. In vitro bacterial adhesion and growth on protamine/HAp was inhibited in a protamine dose-dependent manner. Adherent bacteria exhibited an aberrant morphology for high dosages of protamine/HAp, resulting in the formation of large aggregates and disintegration of the membrane. The released protamine from protamine/HAp also prevented the growth of planktonic bacteria in vitro. However, a high dosage of protamine from powders at loading concentrations over 1000 μg·mL−1 induced a cytotoxic effect in vitro, although those exhibited no apparent cytotoxicity in vivo. These data revealed that protamine/HAp (less than 1000 μg·mL−1) had both antimicrobial activity and biocompatibility and can be applied for bone substitutes in orthopedic fields.

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Section: Resultsmentioning

confidence: 99%

Section: Antimicrobial Evaluationmentioning

confidence: 99%

Potential Application of Protamine for Antimicrobial Biomaterials in Bone Tissue Engineering

Honda

Matsumoto

Aizawa

2020

IJMS

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“…Because THP‐1 cells, a cell line of human monocyte cells, were used in the present study, we could evaluate not only the behavior of cells against the biomaterials but also the behavior of cells involved in the inflammatory immune process for protecting tissues from toxins and initiating the resolution of inflammation 30 . Monocyte recruitment and differentiation may occur in the tissue in response to calcium phosphate‐based biomaterials 31 . Additionally, macrophages are the principal cells responsible for mediating the proinflammatory response toward implant debris as a precursor of osteoclasts 32 …”

Section: Discussionmentioning

confidence: 99%

“…30 Monocyte recruitment and differentiation may occur in the tissue in response to calcium phosphate-based biomaterials. 31 Additionally, macrophages are the principal cells responsible for mediating the proinflammatory response toward implant debris as a precursor of osteoclasts. 32 Previous studies showed the variable effect of the size and shape of nanostructured HA particles on cellular viability.…”

Section: Discussionmentioning

confidence: 99%

Is THP‐1 viability affected by the crystallinity of nanostructured carbonated hydroxyapatites?

Lira

Sartoretto

Pedrosa

et al. 2020

J Biomedical Materials Res

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In daily clinical practice, there is a notable variety of synthetic bone substitute, with various resorption rates, different chemical and structural characteristics that influence on bone regeneration and are not suitable for every clinical use. New biomaterials based on nanotechnology have been developed to be bioabsorbable as new bone is formed. This study intends to evaluate THP‐1 cell viability when exposed to extracts of unsintered nanostructured carbonated hydroxyapatite (cHA) microspheres processed at 5 and 37°C compared to sintered hydroxyapatite processed at 90°C. cHA shows, in previous studies, biocompatibility, and better bioabsorption rates, consequently, improve the deposition of new bone and tissue repair. The results demonstrated that the tested biomaterials did not activate inflammatory role through THP‐1 cells and did not affect activated macrophages independently of their crystallinities, suggesting their safety and biocompatibility. These results are of fundamental importance for the advancement of research on smart materials, especially in what controls the effect of nanostructured cHA microspheres in the biological environment, seems to be a promising biomaterial in clinical application on regenerative medicine.

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“…The process of bone regeneration integrated with bioceramics consists on different steps that can be summarized as a complex and multistage process resulting from a plethora of biological stages; including osteogenesis, angiogenesis and inflammatory responses. All these processes are essential for the recovery of tissue homeostasis and function 13 but for them to occur a good biocompatilibity between the bioceramic and the nearby tissue is essential 12,16 .…”

mentioning

confidence: 99%

Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics

Wijnhoven

Raúl

Santibáñez

et al. 2020

Sci Rep

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The combination of biomaterials and stem cells for clinical applications constitute a great challenge in bone tissue engineering. Hence, cellular networks derived from cells-biomaterials crosstalk have a profound influence on cell behaviour and communication, preceding proliferation and differentiation. The purpose of this study was to investigate in vitro cellular networks derived from human gingival mesenchymal stem cells (hGMSCs) and calcium phosphate (CaP) bioceramic interaction. Biological performance of CaP bioceramic and hGMSCs interaction was evaluated through cell adhesion and distribution, cellular proliferation, and potential osteogenic differentiation, at three different times: 5 h, 1 week and 4 weeks. Results confirmed that hGMSCs met the required MSCs criteria while displaying osteogenic differentiaton capacities. We found a significant increase of cellular numbers and proliferation levels. Also, protein and mRNA OPN expression were upregulated in cells cultured with CaP bioceramic by day 21, suggesting an osteoinductible effect of the CaP bioceramic on hGMSCs. Remarkably, CaP bioceramic aggregations were obtained through hGMSCs bridges, suggesting the in vitro potential of macrostructures formation. We conclude that hGMSCs and CaP bioceramics with micro and macropores support hGMSC adhesion, proliferation and osteogenic differentiation. Our results suggest that investigations focused on the interface cells-biomaterials are essential for bone tissue regenerative therapies.

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Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

Cited by 39 publications

References 280 publications

Potential Application of Protamine for Antimicrobial Biomaterials in Bone Tissue Engineering

Potential Application of Protamine for Antimicrobial Biomaterials in Bone Tissue Engineering

Is THP‐1 viability affected by the crystallinity of nanostructured carbonated hydroxyapatites?

Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics

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