Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics

Wijnhoven, Isabel Benjumeda; Raúl, Vallejos; Santibáñez, Juan F.; Millán, Carola; Vivanco, Juan

doi:10.1038/s41598-020-73428-y

Cited by 13 publications

(5 citation statements)

References 66 publications

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“…Human gingival mesenchymal stem cells (hGMSCs) were used to test the biological performance of the scaffolds [ 37 , 38 ]; they were acquired and cultured following our previously reported methods [ 39 , 40 ]. hGMSCs from primary cell cultures were obtained from human participants after approval by the Institutional Review Board of Universidad Adolfo Ibáñez, IRB-UAI, and the Bioethical Research Committee (approval number 54/2019), as reviewed in Benjumeda et al 2020 [ 37 ]. All experiments associated with cell analysis were performed under the Manual of Biosafety Standards and Associated Risks (Fondecyt-CONICYT 2018) and approved by IRB-UAI.…”

Section: Methodsmentioning

confidence: 99%

Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration

Loyo,

Cordoba,

Palza

et al. 2023

Polymers

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Polymer-based nanocomposites such as polycaprolactone/graphene oxide (PCL/GO) have emerged as alternatives for bone tissue engineering (BTE) applications. The objective of this research was to investigate the impact of a gelatin (Gt) coating on the degradability and different properties of PCL nanofibrous scaffolds fabricated by an electrospinning technique with 1 and 2 wt% GO. Uniform PCL/GO fibers were obtained with a beadless structure and rough surface. PCL/GO scaffolds exhibited an increase in their crystallization temperature (Tc), attributed to GO, which acted as a nucleation agent. Young’s modulus increased by 32 and 63% for the incorporation of 1 and 2 wt% GO, respectively, in comparison with neat PCL. A homogeneous Gt coating was further applied to these fibers, with incorporations as high as 24.7 wt%. The introduction of the Gt coating improved the hydrophilicity and degradability of the scaffolds. Bioactivity analysis revealed that the hydroxyapatite crystals were deposited on the Gt-coated scaffolds, which made them different from their uncoated counterparts. Our results showed the synergic effect of Gt and GO in enhancing the multifunctionality of the PCL, in particular the degradability rate, bioactivity, and cell adhesion and proliferation of hGMSC cells, making it an interesting biomaterial for BTE.

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Section: Methodsmentioning

confidence: 99%

Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration

Loyo,

Cordoba,

Palza

et al. 2023

Polymers

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“…In any case, simply trapping cells at the particular point on a surface is not enough: the cells must be encouraged to differentiate, which is impossible without the presence of suitable biochemical factors [887]. All previously mentioned points clearly indicate that, for the purposes of tissue engineering, CaPO 4 bioceramics play an auxiliary role; namely, they acts as a suitable material to manufacture the appropriate 3D templates, substrates, or scaffolds to be colonized by living cells before the successive implantation [799,800,[888][889][890]. The in vitro evaluation of potential CaPO 4 scaffolds for tissue engineering is described elsewhere [891], while the data on the mechanical properties of CaPO 4 bioceramics for use in tissue engineering are also available [892][893][894].…”

Section: Bioceramic Scaffolds From Capomentioning

confidence: 99%

Calcium Orthophosphate (CaPO4)-Based Bioceramics: Preparation, Properties, and Applications

Dorozhkin¹

2022

Coatings

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Various types of materials have been traditionally used to restore damaged bones. In the late 1960s, a strong interest was raised in studying ceramics as potential bone grafts due to their biomechanical properties. A short time later, such synthetic biomaterials were called bioceramics. Bioceramics can be prepared from diverse inorganic substances, but this review is limited to calcium orthophosphate (CaPO4)-based formulations only, due to its chemical similarity to mammalian bones and teeth. During the past 50 years, there have been a number of important achievements in this field. Namely, after the initial development of bioceramics that was just tolerated in the physiological environment, an emphasis was shifted towards the formulations able to form direct chemical bonds with the adjacent bones. Afterwards, by the structural and compositional controls, it became possible to choose whether the CaPO4-based implants would remain biologically stable once incorporated into the skeletal structure or whether they would be resorbed over time. At the turn of the millennium, a new concept of regenerative bioceramics was developed, and such formulations became an integrated part of the tissue engineering approach. Now, CaPO4-based scaffolds are designed to induce bone formation and vascularization. These scaffolds are usually porous and harbor various biomolecules and/or cells. Therefore, current biomedical applications of CaPO4-based bioceramics include artificial bone grafts, bone augmentations, maxillofacial reconstruction, spinal fusion, and periodontal disease repairs, as well as bone fillers after tumor surgery. Prospective future applications comprise drug delivery and tissue engineering purposes because CaPO4 appear to be promising carriers of growth factors, bioactive peptides, and various types of cells.

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“…Due to its excellent biocompatibility, bioactivity and biodegradability, it has found a wide range of applications in tissue engineering and medicine [17,18]. CaPbased materials promote osteoblast adhesion and proliferation [19,20]. Considering its large surface area and adsorption capacity, it can be a carrier for various drugs, including antibiotics [21,22].…”

Section: Introductionmentioning

confidence: 99%

Influence of Drying Technique on Physicochemical Properties of Synthetic Hydroxyapatite and Its Potential Use as a Drug Carrier

Niziołek,

Słota,

Sadlik

et al. 2023

Materials

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Naturally occurring hydroxyapatite (HA) is the mineral phase of bone tissue. It is characterized by its bioactivity toward stimulating bone cells to proliferate and thus form new apatite layers. For this reason, it is a material commonly used in implantology for filling defects or covering implants (such as endoprostheses). There are several methods to obtain synthetic HA, and by controlling parameters such as temperature, pressure or the drying process, physicochemical parameters of the final powder can be affected. In the present study, HA was obtained by wet precipitation technique and subjected to two different drying methods, determining whether this parameter significantly affects the properties of the final material obtained. Analyzed Fourier-transform infrared spectroscopy (FT-IR) confirmed the presence of functional groups typical for HA. X-ray diffraction analysis (XRD) demonstrated that the materials are partially amorphous; however, the only phase was identified in HA. Scanning electron microscopy (SEM) was used to evaluate the surface morphology and the density, and average grain diameter was measured. Furthermore, HA powders were subjected to modification with the antibiotic clindamycin to determine the potential for use as a carrier for the active substance. The release rate of the drug was determined by high-performance liquid chromatography (HPLC). The differences in the characteristics of the powders were relatively small; however, they affected the rate of drug release from the material as well as the shape of the grains. The method of drying the powders was shown to affect the shape of the grains, as well as the porosity of the sinters prepared from it. A higher amount of clindamycin released into PBS was observed in material with more pores. The materials have demonstrated the potential to be used as a carrier for the active substance; however, further biological, as well as physicochemical, analysis is required.

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Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics

Cited by 13 publications

References 66 publications

Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration

Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration

Calcium Orthophosphate (CaPO4)-Based Bioceramics: Preparation, Properties, and Applications

Influence of Drying Technique on Physicochemical Properties of Synthetic Hydroxyapatite and Its Potential Use as a Drug Carrier

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