The Role of Plant High-Throughput Phenotyping in the Characterization of the Response of High Ascorbate Plants to Abiotic Stresses

Yactayo-Chang, Jessica P.; Acosta‐Gamboa, Lucia M.; Nepal, Nirman; Lorence, Argelia

doi:10.1007/978-3-319-74057-7_13

Cited by 10 publications

(22 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The line used in this study was one of the new ones we developed (Figure a). At MIOX4 line 21 was chosen for this study because it is homozygous, harbors a single copy of the MIOX4 ORF (Figure c), and recapitulates the phenotype of the lines previously reported (Lorence et al, ) that includes elevated levels of AsA (Figure b), enhanced biomass (Figure d) and tolerance to multiple abiotic stresses (Acosta‐Gamboa, ) relative to the wild‐type control (Lisko et al, ; Yactayo‐Chang, ; Yactayo‐Chang et al, ).…”

Section: Resultsmentioning

confidence: 67%

“…The MIOX4 transcript is expressed during reproductive stage in leaf stipules and was found predominantly in cytoplasm and in nucleus (Alford, Rangarajan, Williams, & Gillaspy, ; Endres & Tenhaken, ; Kanter et al, ). Over‐expression of enzymes involved in the myo ‐inositol pathway leads to increased AsA content in Arabidopsis (Lorence et al, ; Radzio et al, ; Yactayo‐Chang, ; Yactayo‐Chang et al, ). This has also been confirmed by other laboratories (Tóth et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…thaliana lines were sensitive to abiotic stresses such as heat, cold, light, and oxidative stress (Conklin et al, ; Pavet et al, ). On the other hand, plants with elevated ascorbate were tolerant to abiotic stresses as heat, cold, drought, salt, and oxidative stress (Eltayeb et al, ; Eltayeb, Fujikawa, & Esaka, ; Lisko et al, ; Tóth et al, ; Yactayo‐Chang et al, ). We found that heat stress response genes ( HSP17 .…”

Section: Discussionmentioning

confidence: 99%

“…The line used in this study was one of the new ones we developed ( Figure 2a). AtMIOX4 line 21 was chosen for this study because it is homozygous, harbors a single copy of the MIOX4 ORF abiotic stresses (Acosta-Gamboa, 2019) relative to the wild-type control (Lisko et al, 2014;Yactayo-Chang, 2011;Yactayo-Chang et al, 2018).…”

Section: Re Sultsmentioning

confidence: 99%

“…Ascorbate scavenges reactive oxygen species (ROS) and protects plants from abiotic and biotic stresses by maintaining ROS homeostasis (Foyer & Noctor, 2011). Plants with elevated AsA content have been developed by over-expressing biosynthetic genes and master regulators (reviewed in Yactayo-Chang, Acosta-Gamboa, Nepal, & Lorence, 2018). Plants with lower vitamin C (vtc mutants) are sensitive to high light, heat, osmotic, and oxidative stresses (Cho et al, 2016;Conklin, DePaolo, Wintle, Schatz, & Buckenmeyer, 2013;Tóth, Nagy, Puthur, Kovacs, & Garab, 2011), but are resistant to Pseudomonas syringae infection due to increased expression of pathogen-related (PR) genes via reduced glutathione accumulation (Pavet et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Mechanisms underlying the enhanced biomass and abiotic stress tolerance phenotype of an Arabidopsis MIOX over‐expresser

et al. 2019

Self Cite

View full text Add to dashboard Cite

Myo ‐inositol oxygenase (MIOX) is the first enzyme in the inositol route to ascorbate (L‐ascorbic acid, AsA, vitamin C). We have previously shown that Arabidopsis plants constitutively expressing MIOX have elevated foliar AsA content and displayed enhanced growth rate, biomass accumulation, and increased tolerance to multiple abiotic stresses. In this work, we used a combination of transcriptomics, chromatography, microscopy, and physiological measurements to gain a deeper understanding of the underlying mechanisms mediating the phenotype of the At MIOX4 line. Transcriptomic analysis revealed increased expression of genes involved in auxin synthesis, hydrolysis, transport, and metabolism, which are supported by elevated auxin levels both in vitro and in vivo, and confirmed by assays demonstrating their effect on epidermal cell elongation in the At MIOX4 over‐expressers. Additionally, we detected up‐regulation of transcripts involved in photosynthesis and this was validated by increased efficiency of the photosystem II and proton motive force. We also found increased expression of amylase leading to higher intracellular glucose levels. Multiple gene families conferring plants tolerance/expressed in response to cold, water limitation, and heat stresses were found to be elevated in the At MIOX4 line. Interestingly, the high AsA plants also displayed up‐regulation of transcripts and hormones involved in defense including jasmonates, defensin, glucosinolates, and transcription factors that are known to be important for biotic stress tolerance. These results overall indicate that elevated levels of auxin and glucose, and enhanced photosynthetic efficiency in combination with up‐regulation of abiotic stresses response genes underly the higher growth rate and abiotic stresses tolerance phenotype of the At MIOX4 over‐expressers.

show abstract

Section: Resultsmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Re Sultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mechanisms underlying the enhanced biomass and abiotic stress tolerance phenotype of an Arabidopsis MIOX over‐expresser

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

Phenotypic characterization of Arabidopsis thaliana lines overexpressing AVP1 and MIOX4 in response to abiotic stresses

et al. 2020

Self Cite

View full text Add to dashboard Cite

Premise AVP1 (H+‐pyrophosphatase) and MIOX4 (myo‐inositol oxygenase) are genes that, when overexpressed individually, enhance the growth and abiotic stress tolerance of Arabidopsis thaliana plants. We propose that pyramiding AVP1 and MIOX4 genes will further improve stress tolerance under water‐limited and salt‐stress conditions. Methods MIOX4 and AVP1 reciprocal crosses were developed and phenomic approaches used to investigate the possible synergy between these genes. Results Under normal and stress conditions, the crosses had higher foliar ascorbate content than the wild‐type and parental lines. Under water‐limited conditions, the crosses also displayed an enhanced growth rate and biomass compared with the control. The observed increases in photosystem II efficiency, linear electron flow, and relative chlorophyll content may have contributed to this observed phenotype. Additionally, the crosses retained more water than the controls when subjected to salt stress. Higher seed yields were also observed in the crosses compared with the controls when grown under salt and water‐limitation stresses. Discussion Overall, these results suggest the combinatorial effect of overexpressing MIOX4 and AVP1 may be more advantageous than the individual traits for enhancing stress tolerance and seed yields during crop improvement.

show abstract

Morphological, phenotypic and nutritional characterization of exotic and local tomato germplasm under tunnel

Fozia,

Zahid,

Gao

et al. 2024

Food Bioscience

View full text Add to dashboard Cite

The Role of Plant High-Throughput Phenotyping in the Characterization of the Response of High Ascorbate Plants to Abiotic Stresses

Cited by 10 publications

References 86 publications

Mechanisms underlying the enhanced biomass and abiotic stress tolerance phenotype of an Arabidopsis MIOX over‐expresser

Mechanisms underlying the enhanced biomass and abiotic stress tolerance phenotype of an Arabidopsis MIOX over‐expresser

Phenotypic characterization of Arabidopsis thaliana lines overexpressing AVP1 and MIOX4 in response to abiotic stresses

Morphological, phenotypic and nutritional characterization of exotic and local tomato germplasm under tunnel

Contact Info

Product

Resources

About