Mechanisms underlying the enhanced biomass and abiotic stress tolerance phenotype of an Arabidopsis MIOX over‐expresser

Nepal, Nirman; Yactayo-Chang, Jessica P.; Medina‐Jiménez, Karina; Acosta‐Gamboa, Lucia M.; González-Romero, M E; Arteaga-Vázquez, Mario; Lorence, Argelia

doi:10.1002/pld3.165

Cited by 28 publications

(29 citation statements)

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“…The expression of MIOX4 or AVP1 enhances the abiotic stress tolerance of transgenic plants. Transcriptomic studies of MIOX4 OE plants revealed that AVP1 expression is independent of MIOX4 expression (Nepal et al, 2019); therefore, we hypothesize that the co-expression of AVP1 and MIOX4 will further improve abiotic stress tolerance. Here, we use high-throughput phenotyping approaches and real-time quantitative PCR (qPCR) to characterize the additive effects of AVP1 and MIOX4 in A. thaliana exposed to water-limitation and salt stress.…”

mentioning

confidence: 88%

“…Seeds of the A. thaliana AVP1 OE line (AVP1-1) were provided by R.G. The A. thaliana MIOX4 OE line was developed as described by Nepal et al (2019). The seeds were surface-sterilized http://www.wileyonlinelibrary.com/journal/AppsPlantSci © 2020 Nepal et al and the AVP1 OE, MIOX4 OE, and crosses (described below) were sown on Murashige and Skoog (MS) medium (Murashige and Skoog, 1962) plus kanamycin, while the WT Col-0 controls were sown on MS medium.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…Arabidopsis thaliana leaves were collected for ascorbate measurement when the plants were in developmental stage 6.1 for the salt stress experiment, and developmental stage 6.3 for the water-limitation stress, according to the nomenclature presented by Boyes et al (2001). The ascorbate measurements were performed using an enzyme-based spectrophotometric method as described previously (Nepal et al, 2019).…”

Section: Ascorbate Measurementsmentioning

confidence: 99%

“…Three biological replicates of foliar samples were collected at developmental stages 6.1 and 6.3 for the plants under the salt stress and water-limitation stress experiments, respectively. The total RNA extraction, quality analysis, and cDNA synthesis were performed as previously described (Nepal et al, 2019). The efficiencies of the primers for amplifying the AVP1 and MIOX4 genes were calculated using the MIQE guidelines (Bustin et al, 2009).…”

Section: Rna Extraction and Quantitative Pcrmentioning

confidence: 99%

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Phenotypic characterization of Arabidopsis thaliana lines overexpressing AVP1 and MIOX4 in response to abiotic stresses

et al. 2020

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Premise AVP1 (H+‐pyrophosphatase) and MIOX4 (myo‐inositol oxygenase) are genes that, when overexpressed individually, enhance the growth and abiotic stress tolerance of Arabidopsis thaliana plants. We propose that pyramiding AVP1 and MIOX4 genes will further improve stress tolerance under water‐limited and salt‐stress conditions. Methods MIOX4 and AVP1 reciprocal crosses were developed and phenomic approaches used to investigate the possible synergy between these genes. Results Under normal and stress conditions, the crosses had higher foliar ascorbate content than the wild‐type and parental lines. Under water‐limited conditions, the crosses also displayed an enhanced growth rate and biomass compared with the control. The observed increases in photosystem II efficiency, linear electron flow, and relative chlorophyll content may have contributed to this observed phenotype. Additionally, the crosses retained more water than the controls when subjected to salt stress. Higher seed yields were also observed in the crosses compared with the controls when grown under salt and water‐limitation stresses. Discussion Overall, these results suggest the combinatorial effect of overexpressing MIOX4 and AVP1 may be more advantageous than the individual traits for enhancing stress tolerance and seed yields during crop improvement.

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confidence: 88%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Section: Ascorbate Measurementsmentioning

confidence: 99%

Section: Rna Extraction and Quantitative Pcrmentioning

confidence: 99%

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Phenotypic characterization of Arabidopsis thaliana lines overexpressing AVP1 and MIOX4 in response to abiotic stresses

et al. 2020

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“…Studies have shown that MIOX plays a role in the response to environmental stresses. In Arabidopsis, MIOX is encoded by a gene family of four members, it has been reported that Arabidopsis MIOX4 over express can increase the tolerance to cold, heat and salt [3,[6][7][8]. In addition, it has also been reported that rice MIOX has a speci c function in drought stress tolerance by decreasing oxidative damage [9].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of The MIOX Gene Family in Cotton

Li¹,

Liu²,

Wei³

et al. 2020

Preprint

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Background: The enzyme myo-inositol oxygenase (MIOX) catalyzes the myo-inositol into glucuronic acid. Previous studies indicated that MIOX may play an important role in plant responses to abiotic stresses. Cotton is a major source of natural and renewable textile. However, the MIOX gene family of cotton has not been systematically identified and characterized yet.Results: In this study, 6 MIOX genes were identified from all of the three diploid cotton species (Gossypium arboretum, Gossypium herbaceum and Gossypium raimondii), 12 MIOX genes were identified from two domesticated tetraploid cottons Gossypium hirsutum, Gossypium barbadense, and 11 MIOX genes were identified from three wild tetraploid cottons Gossypium tomentosum, Gossypium mustelinum and Gossypium darwinii. The number of MIOX genes in tetraploid cotton genome is roughly twice that of diploid cotton genome. Members of MIOX family were classified into six groups based on the phylogenetic analysis. Integrated analysis of collinearity events and chromosome locations suggested that both whole genome duplication and segmental duplication events contributed to the expansion of MIOX genes during cotton evolution. The ratios of non-synonymous (Ka) and synonymous (Ks) substitution rates revealed that purifying selection was the main force driving the evolution of MIOX genes. Numerous cis-acting elements related to light responsive element, defense and stress responsive element were identified in the promoter of the MIOX genes. Expression analyses of MIOX genes based on RNA-seq data showed that MIOX genes within the same group shared similar expression patterns with each other.Conclusions: In this work, we systematically analyzed MIOX genes from eight Gossypium genomes and the Gossypioides kirkii genome using a set of bioinformatics approaches. All of these results provide the foundation for further study of the biological functions of MIOX genes in cotton environmental adaptability.

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Myo‐inositol oxygenase (MIOX) accelerated inflammation in the model of infection‐induced cardiac dysfunction by NLRP3 inflammasome

Zhou

Long

2023

Immunity Inflam & Disease

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Background Cardiac dysfunction is an important component of multiple organ failure caused by sepsis, and an important cause of high mortality in patients with sepsis. Herein, we attempted to determine whether myo‐inositol oxygenase (MIOX) has proinflammation enzyme in infection‐induced cardiac dysfunction (IICD) and its underlying mechanism. Methods Patients with IICD were collected by our hospital. A mouse model of IICD was induced into male db/db mice by cecal ligation and puncture (CLP). All mice were injected with 20 μL of LV‐MIOX or LV‐control short hairpin RNA using a 0.5‐mL insulin syringe. On the second day, all mice were induced by CLP. H9C2 cell was also induced with lipopolysaccharide and adenosine triphosphate. Quantitative analysis of messenger RNAs (mRNAs) and gene microarray hybridization was used to analyze the mRNA expression levels. Enzyme‐linked immunosorbent assay, immunofluorescence, and Western blot analysis were used to analyze the protein expression levels. Results The serum expressions of MIOX mRNA level in patients with IICD were upregulated compared to normal healthy volunteers. MIOX promoted inflammation levels in the in vitro model of IICD. Si‐MIOX inhibited inflammation levels in the in vitro model of IICD. MIOX accelerated inflammation and cardiac dysfunction in infection‐induced mice. MIOX interacted with NLR family pyrin domain containing 3 (NLRP3) protein to reduce the degradation of NLRP3. The inhibition of MIOX reversed the effects of NLRP3 in the in vitro model of cardiac dysfunction. Conclusions Taken together, these findings demonstrate that MIOX accelerates inflammation in the model of IICD, which may be, at least in part, attributable to NLRP3 activity by the suppression of NLRP3 degradation in IICD.

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Mechanisms underlying the enhanced biomass and abiotic stress tolerance phenotype of an Arabidopsis MIOX over‐expresser

Cited by 28 publications

References 136 publications

Phenotypic characterization of Arabidopsis thaliana lines overexpressing AVP1 and MIOX4 in response to abiotic stresses

Phenotypic characterization of Arabidopsis thaliana lines overexpressing AVP1 and MIOX4 in response to abiotic stresses

Genome-Wide Identification and Characterization of The MIOX Gene Family in Cotton

Myo‐inositol oxygenase (MIOX) accelerated inflammation in the model of infection‐induced cardiac dysfunction by NLRP3 inflammasome

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