The Role of Physical Factors in Cell Differentiation, Tissue Repair and Regeneration

Monici, Monica; Cialdai, Francesca

doi:10.5772/26014

Cited by 10 publications

(11 citation statements)

References 78 publications

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“…Mechanical properties influence cell function, differentiation, morphology, and composition (Blacklock et al 2010;Monici and Cialdai 2008;Wells 2008). Nanofiber is suitable for being used as a scaffold because it has same morphological features with extracellular matrix (ECM).…”

Section: Introductionmentioning

confidence: 99%

Regulation of astrocyte activity via control over stiffness of cellulose acetate electrospun nanofiber

Min

Jung

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

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Astrocytes are involved in neuron protection following central nervous system (CNS) injury; accordingly, engineered astrocytes have been investigated for their usefulness in cell therapy for CNS injury. Nanofibers have attracted a great deal of attention in neural tissue engineering, but their mechanical properties greatly influence physiology. Cellulose acetate (CA) has been studied for use in scaffolds owing to its biocompatibility, biodegradability, and good thermal stability. In this study, stiffness of CA nanofibers controlled by heat treatment was shown to regulate astrocyte activity. Adhesion and viability increased in culture as substrate became stiffer but showed saturation at greater than 2 MPa of tensile strength. Astrocytes became more active in terms of increasing intermediate filament glial fibrillary acidic protein (GFAP). The results of this study demonstrate the effects of stiffness alone on cellular behaviors in a three-dimensional culture and highlight the efficacy of heat-treated CA for astrocyte culture in that the simple treatment enables control of astrocyte activity.

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Section: Introductionmentioning

confidence: 99%

Regulation of astrocyte activity via control over stiffness of cellulose acetate electrospun nanofiber

Min

Jung

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

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“…It is noteworthy that, in the case of FN, not only the expression returned to levels comparable with unstimulated controls, but in samples treated with laser radiation the fibrils showed a more ordered and parallel distribution. This effect of laser radiation on FN and collagen fibril organization has already been described [ 73 ] and could be connected with the laser radiation’s ability to prevent fibrotic scars. The influence of red-NIR laser radiation on the expression of α5β1 integrin, FN, collagen, and MMP-1 has already been investigated in studies concerning laser application in the management of inflammatory response and wound healing.…”

Section: Discussionmentioning

confidence: 59%

Effect of NIR Laser Therapy by MLS-MiS Source on Fibroblast Activation by Inflammatory Cytokines in Relation to Wound Healing

et al. 2021

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The fine control of inflammation following injury avoids fibrotic scars or impaired wounds. Due to side effects by anti-inflammatory drugs, the research is continuously active to define alternative therapies. Among them, physical countermeasures such as photobiomodulation therapy (PBMT) are considered effective and safe. To study the cellular and molecular events associated with the anti-inflammatory activity of PBMT by a dual-wavelength NIR laser source, human dermal fibroblasts were exposed to a mix of inflammatory cytokines (IL-1β and TNF-α) followed by laser treatment once a day for three days. Inducible inflammatory key enzymatic pathways, as iNOS and COX-2/mPGES-1/PGE2, were upregulated by the cytokine mix while PBMT reverted their levels and activities. The same behavior was observed with the proangiogenic factor vascular endothelial growth factor (VEGF), involved in neovascularization of granulation tissue. From a molecular point of view, PBMT retained NF-kB cytoplasmatic localization. According to a change in cell morphology, differences in expression and distribution of fundamental cytoskeletal proteins were observed following treatments. Tubulin, F-actin, and α-SMA changed their organization upon cytokine stimulation, while PBMT reestablished the basal localization. Cytoskeletal rearrangements occurring after inflammatory stimuli were correlated with reorganization of membrane α5β1 and fibronectin network as well as with their upregulation, while PBMT induced significant downregulation. Similar changes were observed for collagen I and the gelatinolytic enzyme MMP-1. In conclusion, the present study demonstrates that the proposed NIR laser therapy is effective in controlling fibroblast activation induced by IL-1β and TNF-α, likely responsible for a deleterious effect of persistent inflammation.

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“…In the light of the present research, it is significant that the MLS M1 system provides the possibility of simultaneous emission of both wavelengths of laser radiation (808 nm—continuously, 905 nm in a continuous or pulsed emission) . The in vitro findings demonstrate that combined emission stimulates cell metabolism in a way that is substantially independent from the dose and time, and ensures the highest biostimulation effect both in extent and duration …”

Section: Discussionmentioning

confidence: 61%

“…The in vitro findings demonstrate that combined emission stimulates cell metabolism in a way that is substantially independent from the dose and time, and ensures the highest biostimulation effect both in extent and duration. [64] The two wavelengths emitted at the same time are perceived by the cell as a new unknown stimulus, which can cause a characteristic biological effect that cannot be obtained by the emission of each wave separately. This method of emission provides the tissues with energy to produce the maximum biological effect.…”

Section: Discussionmentioning

confidence: 99%

Effect of Photobiomodulation Therapy on the Increase of Viability and Proliferation of Human Mesenchymal Stem Cells

et al. 2019

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Background and Objectives We have investigated how low intensity laser irradiation emitted by a multiwave‐locked system (MLS M1) affects the viability and proliferation of human bone marrow mesenchymal stem cells (MSCs) depending on the parameters of the irradiation. Study Design/Materials and Methods Cells isolated surgically from the femoral bone during surgery were identified by flow cytometry and cell differentiation assays. For irradiation, two wavelengths (808 and 905 nm) with the following parameters were used: power density 195, 230, and 318 mW/cm 2, doses of energy 3, 10, and 20 J (energy density 0.93–6.27 J/cm 2), and in continuous (CW) or pulsed emission (PE) (frequencies 1,000 and 2,000 Hz). Results There were statistically significant increases of cell viability and proliferation after irradiation at 3 J (CW; 1,000 Hz), 10 J (1,000 Hz), and 20 J (2,000 Hz). Conclusions Irradiation with the MLS M1 system can be used in vitro to modulate MSCs in preparation for therapeutic applications. This will assist in designing further studies to optimize the radiation parameters and elucidate the molecular mechanisms of action of the radiation. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

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The Role of Physical Factors in Cell Differentiation, Tissue Repair and Regeneration

Cited by 10 publications

References 78 publications

Regulation of astrocyte activity via control over stiffness of cellulose acetate electrospun nanofiber

Regulation of astrocyte activity via control over stiffness of cellulose acetate electrospun nanofiber

Effect of NIR Laser Therapy by MLS-MiS Source on Fibroblast Activation by Inflammatory Cytokines in Relation to Wound Healing

Effect of Photobiomodulation Therapy on the Increase of Viability and Proliferation of Human Mesenchymal Stem Cells

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