Effect of Photobiomodulation Therapy on the Increase of Viability and Proliferation of Human Mesenchymal Stem Cells

Pasternak-Mnich, Kamila; Ziemba, Barbara; Szwed, Aleksandra; Kopacz, Karolina; Synder, Marek; Bryszewska, Maria; Kujawa, Jolanta

doi:10.1002/lsm.23107

Cited by 14 publications

(9 citation statements)

References 60 publications

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“…BMSCs are MSCs readily isolated from the bone marrow that can support angiogenesis, enhance secretion of nerve growth factors (NGFs), restore nerve function after ischemic stroke, and treat non-healing fractures. 34 Many studies point to the potential BMSCs have for wound healing, fat production, and bone formation. However, regarding these applications of PBM on BMSCs, wavelengths in the near-infrared regions (808 and 890 nm) produced more significant and remarkable results.…”

Section: Bone Marrow-derived Stem Cells (Bmscs)mentioning

confidence: 99%

Photobiomodulation improves the therapeutic efficacy of mesenchymal stem cells in regenerative medicine

Woo¹,

Abueva²

2022

Medical Lasers

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Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into many other cell types typically isolated from adult tissue sources. The therapeutic potential of MSCs is considerable in regenerative medicine and has advanced over the years. Consequently, methods and tools that improve the therapeutic efficacy of MSCs have also been devised and utilized. Recent strategies used to enhance the therapeutic efficacy of MSC include photobiomodulation (PBM), which can increase proliferation and enhance differentiation into target cell types. PBM is a non-thermal, non-invasive method that utilizes light from coherent or non-coherent sources to influence metabolic activity at the cellular level and has been found to increase cell proliferation and migration in vitro, in vivo, and in clinical studies. However, little is known of the mechanism responsible for the effects of PBM on MSCs or its potential as an adjunctive treatment, which obscures appreciation of its regenerative applications. This review summarizes reports on the effects of PBM on different MSCs and provides perspectives on the ability of PBM to improve the therapeutic efficacy of MSCs in regenerative medicine.

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Section: Bone Marrow-derived Stem Cells (Bmscs)mentioning

confidence: 99%

Photobiomodulation improves the therapeutic efficacy of mesenchymal stem cells in regenerative medicine

Woo¹,

Abueva²

2022

Medical Lasers

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“…65 A recent study demonstrated that PBMT can act on migration, differentiation and production of extracellular matrix molecules by progenitor cells. 66 Based on the results of in vitro studies, significant evidence of an upregulation of the regenerative capacity of MSCs has been accumulated. The combined use of BMA plus PBMT seems to be a good strategy in the clinical scenario.…”

Section: Enhancement Of the Bma Clotmentioning

confidence: 99%

Bone marrow aspirate clot: A feasible orthobiologic

Lana¹,

Fonseca

Mosaner³

et al. 2020

Journal of Clinical Orthopaedics and Trauma

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Musculoskeletal disorders are one of the major health burdens and a leading source of disability worldwide, affecting both juvenile and elderly populations either as a consequence of ageing or extrinsic factors such as physical injuries. This condition often involves a group of locomotor structures such as the bones, joints and muscles and may therefore cause significant economic and emotional impact. Some pharmacological and non-pharmacological treatments have been considered as potential solutions, however, these alternatives have provided quite limited efficacy due to the short-term effect on pain management and inability to restore damaged tissue.The emergence of novel therapeutic alternatives such as the application of orthobiologics, particularly bone marrow aspirate (BMA) clot, have bestowed medical experts with considerable optimism as evidenced by the significant results found in numerous studies addressed in this manuscript. Although other products have been proposed for the treatment of musculoskeletal injuries, the peculiar interest in BMA, fibrin clot and associated fibrinolytic mechanisms continues to expand.BMA is a rich source of various cellular and molecular components which have demonstrated positive effects on tissue regeneration in many in vitro and in vivo models of musculoskeletal injuries. In addition to being able to undergo self-renewal and differentiation, the hematopoietic and mesenchymal stem cells present in this orthobiologic elicit key immunomodulatory and paracrine roles in inflammatory responses in tissue injury and drive the coagulation cascade towards tissue repair via different mechanisms.Although promising, these complex regenerative mechanisms have not yet been fully elucidated.

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“…The treatment method includes low-level laser therapy and laser acupuncture [ 12 , 13 ]. Studies indicate that laser acupuncture for Bell's palsy can reduce pain and have anti-inflammatory effects, though the exact mechanism remains unclear [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Laser acupuncture and photobiomodulation therapy in Bell’s palsy with a duration of greater than 8 weeks: a randomized controlled trial

Wu,

Lan,

Litscher

et al. 2024

Lasers Med Sci

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To investigate the efficacy of laser acupuncture and photobiomodulation therapy in alleviating symptoms among patients diagnosed with Bell’s palsy with duration of greater than 8 weeks. The randomized controlled trial has been performed from May 2021 to April 2023. Patients were eligible who had Bell’s palsy with duration of greater than 8 weeks on out-patient Department of Otorhinolaryngology in Beijing Tongren Hospital. The laser acupuncture group received class IV laser treatment for 3 times per weeks, a total of 72 times. The control group received the same treatment procedure except the laser parameter. The primary outcome measures comprised House–Brackmann facial nerve grading system and electroneurography. Secondary outcome measures comprised Sunnybrook facial grading system, electromyography, and the blink reflex. A total of 84 participants were included (42 control group, 42 laser acupuncture group). After treatment, House–Brackmann facial nerve grading system (OR, 0.11; 95% CI, 0.04–0.30; P < 0.001), and the pathologic numbers of electroneuronography were statistically different between the laser acupuncture group and control group, including orbicularis oculi (OR,0.08; 95% CI, 0.02–0.21; P < 0.001), Frontalis muscle (OR,0.14; 95% CI, 0.05–0.39; P < 0.001), Orbicularis oris (OR,0.13; 95% CI, 0.04–0.36; P < 0.001), Ala nasi muscle (OR,0.06; 95% CI, 0.02–0.18; P < 0.001). In secondary outcomes, Sunnybrook facial grading system, has significant difference between the two groups (20.26; 95% CI, 14.69 to 25.83; P < 0.01). Latency by ENoG, include orbicularis oculi (-0.61; 95% CI, -0.43 to -0.09; P < 0.001), frontalis muscle (-0.12; 95% CI, -0.21 to -0.03; P < 0.01), orbicularis oris (-0.28; 95% CI, -0.41 to -0.16; P < 0.001), and ala nasi muscle (-0.26; 95% CI, -0.38 to -0.16; P < 0.001). All amplitudes of MUAPs and durations by electromyography (EMG) showed statistically significant differences compared with the control group after treatment. For the frontalis muscle, the amplitude of MUAPs was -64.23 (95% CI, -80.89 to -47.56; P < 0.001) and duration was -1.18 (95% CI, -1.49 to -0.87; P < 0.001). For orbicularis oris, amplitude of MUAPs was -29.82 (95% CI, -55.03 to -4.62; P = 0.02) and duration was -0.57 (95% CI, -0.94 to -0.20; P < 0.001). For depressor angulli oris, amplitude of MUAPs was -47.06 (95% CI, -62.15 to -31.97; P < 0.001) and duration was -2.21 (95% CI, -2.69 to -1.72; P < 0.001). Blink reflex, including R1 (OR, 0.03; 95% CI, 0.01–0.16; P < .001), R2 (OR, 0.04; 95% CI, 0.004–0.29; P < .001), and R2 latency differences (OR, 0.15; 95% CI, 0.05–0.51; P < .001), have significant difference between the two groups, respectively. The findings suggest that laser acupuncture relieve symptoms for patients with Bell’s palsy with a duration of greater than 8 weeks.Trial registration: ClinicalTrials.gov Identifier: NCT05846217.

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Effect of Photobiomodulation Therapy on the Increase of Viability and Proliferation of Human Mesenchymal Stem Cells

Cited by 14 publications

References 60 publications

Photobiomodulation improves the therapeutic efficacy of mesenchymal stem cells in regenerative medicine

Photobiomodulation improves the therapeutic efficacy of mesenchymal stem cells in regenerative medicine

Bone marrow aspirate clot: A feasible orthobiologic

Laser acupuncture and photobiomodulation therapy in Bell’s palsy with a duration of greater than 8 weeks: a randomized controlled trial

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