The role of physical and chemical characteristics in assessing the performance of a new biological vascular graft

Roberts, G. R.; McCormack, H.; Ketharanathan, V.; Macleish, D. G.; Field, P. L.; Milne, P. Y.

doi:10.1002/jbm.820230405

Cited by 11 publications

(7 citation statements)

References 5 publications

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“…The pore architecture of an acellular scaffold depends critically on the donor; the tissue source of the ECM; the method of decellularization; the geometry, thickness, and orientation of the scaffold; and the extent of enzymatic and chemical treatment, among other factors. [13][14][15][16][17] Previous studies have also shown that, with proper treatment, the pore architecture of the acellular scaffolds could be improved. For example, Wei et al 16 demonstrated that the pore size of acellular bovine pericardia more than tripled when treated with acetic acid or collagenase, and the porosities increased from 63.4% to greater than 90%.…”

Section: Introductionmentioning

confidence: 94%

Pore Architecture of a Bovine Acellular Vocal Fold Scaffold

Chan

2008

Tissue Engineering Part A

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An acellular xenogeneic scaffold derived from the bovine vocal fold lamina propria has shown some promise for in vitro vocal fold tissue engineering. To further explore the potential of the scaffold for cellular attachment, migration, and infiltration, as well as the transport of oxygen, proteins, and nutrients in vivo, this study examined the architecture of pores in the scaffold in terms of several key parameters. Porosity was determined using a standard fluid replacement method with a pycnometer. Average pore size and the pore size distribution were assessed using digital image analysis of scanning electron micrographs. The intrinsic permeability to water was measured using a custom-built hydrostatic pressure apparatus as an estimation of the overall porous nature of the acellular scaffold. The results indicated that the bovine acellular scaffold has a reasonably high porosity (90.49 +/- 4.33%), a proper pore size distribution (>60% of the pores with equivalent diameters > or =10 microm and < 100 microm) that could facilitate cellular attachment and infiltration, as well as a relatively high intrinsic permeability (0.21-3.21 darcy) for the transport of soluble factors. These findings offered preliminary support of the potential of the scaffold for facilitating functional extracellular matrix remodeling in vocal fold reconstruction.

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Section: Introductionmentioning

confidence: 94%

Pore Architecture of a Bovine Acellular Vocal Fold Scaffold

Chan

2008

Tissue Engineering Part A

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“…The CTBU vascular prosthesis is a tubular biological material of non-vascular origin, with a unified wall structure, which has been further strengthened by collagen cross-linking using glutaraldehyde. In accelerated fatigue testing [10], the prosthesis has been shown not to weaken or demonstrate changes indicative of aneurysm formation when subjected to a pulsatile flow of 135 beats/min at a pulse pressure of 0 to 25 kPa for 96 h. The HUVG (Meadox Medicals Inc., Oakland, NJ) and the bovine heterograft (Soleo Basle Ltd., Switzerland) on the other hand both exhibited greater wall changes following accelerated fatigue tests than the CTBU.…”

Section: Discussionmentioning

confidence: 99%

“…The bovine ureters were collected from Australian cattle, which are free of bovine spongiform encepha-lo~athy (BSE) [12], at a Government certified abattoir, Following treatment with 2% glutaraldehyde for a maximum of 72 h, the processed ureters were tested for mural integrity by measuring porosity after submitting the prosthesis to a pressure of 25 kPa for 1 min [10]. They were then individually packed in glass tubes, chemically sterilised in 1% glutaraldehyde, nnsed and finally stored in 50% ethanol ready for use.…”

Section: Methodsmentioning

confidence: 99%

“…Sterility of each batch was confirmed at the completion of the process. The methods and results of physical and chemical evaluation of CTBU were based in part on the American National Standard for Vascular Graft Prostheses [13], and are documented by Roberts et al [10] and Bums et al l11]. Ketharanathan and others have reported the subsequent animal work [8][9][10][11].…”

Section: Methodsmentioning

confidence: 99%

“…The chemically treated bovine ureter--</inical performance of a novel biological vascular prosthesis: P. L. Field ine ureter (CTBU) (Flonova", Bionova International Pty Ltd, Melbourne). This has been developed and evaluated in Australia over 20 years, refined through animal work previously reported, and is intended as a coronary or peripheral artery substitute [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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The Chemically Treated Bovine Ureter—Clinical Performance of a Novel Biological Vascular Prosthesis

Field

2003

Cardiovascular Surgery

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Aim This paper describes the rationale, development and clinical performance of a novel small-diameter vascular prosthesis of non-vascular animal tissue origin. Method Durable conduits suitable for coronary and peripheral arterial reconstruction are prepared for the first time from non-vascular animal tissue. Australian bovine ureters from young steers from protected herds are processed to form strong, bloodflow-compatible and non-immunogenic biologic grafts (‘Flonova’ R, Bionova International Pty Ltd, Melbourne). Fifty-six patients lacking suitable autogenous veins received 62 prostheses (32 bypasses were implanted above knee and 30 below knee). Determination of patency and function included Doppler ultrasound, duplex scanning and angiography. Findings The primary patency rate at 1, 2 and 5 years, respectively was 67, 59 and 52% and the secondary patency for the same time frames was 88, 73 and 61%. There were no occlusions as a result of intimal hyperplasia. Dilatation of the wall was diagnosed in one prosthesis at 3.5 years, and one at 7 years, and a false aneurysm of unknown aetiology occurred in another at 3 months. There were no primary graft infections. Conclusion This evaluation shows the chemically treated bovine ureter (CTBU) is a viable medium-term alternative to the currently available artificial vascular prostheses for peripheral revascularisation.

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Porosity of porcine small‐intestinal submucosa for use as a vascular graft

Hiles

Badylak

Geddes

et al. 1993

J. Biomed. Mater. Res.

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The porosity of a vascular graft material has been suggested as a major factor affecting the rate and degree of neovascularization of newly implanted grafts, with higher porosities generally associated with better performance. The objective of this study was to determine the water porosity of a new vascular graft material, small-intestinal submucosa (SIS), and to compare the values to those reported for other common vascular graft materials. In addition, the porosity of SIS was investigated with respect to applied pressure and applied uniaxial tension. Both rectangular, flat specimens and tubular specimens of SIS were subjected to static water pressure, and water was collected as it passed through the SIS material. SIS has a typical porosity of 0.52 mL/min.cm-2 at an applied pressure of 120 mm Hg. Although porosity appeared to be unaffected by uniaxial tension, it increased in proportion to applied pressure at a rate of 4.8 x 10(-3) mL/min.cm-2/mm Hg. These low porosity values and the past success of SIS as a vascular graft material suggest that high-porosity materials are not required for implant success.

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The role of physical and chemical characteristics in assessing the performance of a new biological vascular graft

Cited by 11 publications

References 5 publications

Pore Architecture of a Bovine Acellular Vocal Fold Scaffold

Pore Architecture of a Bovine Acellular Vocal Fold Scaffold

The Chemically Treated Bovine Ureter—Clinical Performance of a Novel Biological Vascular Prosthesis

Porosity of porcine small‐intestinal submucosa for use as a vascular graft

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