Porosity of porcine small‐intestinal submucosa for use as a vascular graft

Abstract

The porosity of a vascular graft material has been suggested as a major factor affecting the rate and degree of neovascularization of newly implanted grafts, with higher porosities generally associated with better performance. The objective of this study was to determine the water porosity of a new vascular graft material, small-intestinal submucosa (SIS), and to compare the values to those reported for other common vascular graft materials. In addition, the porosity of SIS was investigated with respect to applied pressure and applied uniaxial tension. Both rectangular, flat specimens and tubular specimens of SIS were subjected to static water pressure, and water was collected as it passed through the SIS material. SIS has a typical porosity of 0.52 mL/min.cm-2 at an applied pressure of 120 mm Hg. Although porosity appeared to be unaffected by uniaxial tension, it increased in proportion to applied pressure at a rate of 4.8 x 10(-3) mL/min.cm-2/mm Hg. These low porosity values and the past success of SIS as a vascular graft material suggest that high-porosity materials are not required for implant success.

Development and Characterization of Acellular Extracellular Matrix Scaffolds from Porcine Menisci for Use in Cartilage Tissue Engineering

“…Our studies also found that the collagen content was half of that of fresh menisci when treated with acetic acid for 2 h. These results indicate that acetic acid is not an ideal decellularization reagent due to its negative influence on collagen. In addition, peracetic acid is a common decellularization agent for porcine SIS and layers of the urinary bladder, 52,53 and it was reported to be highly efficient at removing residual nucleic acids from thin tissues with minimal effects on the ECM composition at concentrations of 0.10-0.15%. 30 However, here 0.15% peracetic had a limited decellularization effect on menisci in the first 2 h; furthermore, 15% peracetic acid treatment for 10 h decreased the DNA content to 4.68% -0.01%; while the amounts of collagen and GAG were respectively reduced to 44.98% -3.00% and 19.04% -4.01% compared to fresh menisci.…”

Intestine Submucosa and Polypropylene Mesh for Abdominal Wall Repair in Dogs

“…The final repair thickness was apparently necessary to maintain body wall integrity during re-porosity to water in that the submucosa to stratum compactum direction is four times more porous than modeling and SIS resorption. The SIS/connective tissue repairs were smooth and confluent with the adjacent the opposite direction [27,28]. It is not known at this time if this characteristic would alter tissue ingrowth.…”

Pore Architecture of a Bovine Acellular Vocal Fold Scaffold

“…24 In the tissueengineering literature, the term ''porosity'' has sometimes been used to refer to this concept of permeability, 14,18,25 which is misleading. In the present study, the permeability of the bovine acellular scaffold was assessed as an indication of the overall porous nature of the material microstructure.…”