Coastal protection, nutrient cycling, erosion control, water purification, and carbon sequestration are ecosystem services provided by salt marshes. Additionally, the salt ponds offers coastal breeding and nursery habitat to fishes and invertebrates providing abundant amphipods potentially useful as a resource in aquaculture. Fishmeal and fish oil are necessary food resources to support aquaculture of carnivorous species due to their omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA). Their dependence on limited fisheries constrains growth of aquaculture and the need for n-3 LC-PUFA recommends the development of more sustainable food sources. Due to their extensive feeding on detritus and primary producers, amphipods appear as potential and high quality alternative feed resource for aquaculture. Hence, a nutritional study was carried out for main amphipods species -Microdeutopus gryllotalpa, Monocorophium acherusicum, Gammarus insensibilis, Melita palmata and Cymadusa filosa-at South Spain aquaculture terrestrial ponds . These species showed a high protein content (up to 40%), high n-3 PUFA and phospholipid levels, and high levels of phophatidylcholine (PC), phosphatidylethanolamine (PE) and triacylglycerols(TAG), the latter significantly high for M. acherusicum . M. gryllotalpa and M. acherusicum showed the highest proportion of lipids (19.15% and 18.35%, respectively). Isoleucine, glycine and alanine were the dominant amino acids in all species. In addition, amphipods collected from ponds showed low levels of heavy metals. Furthermore, the biochemical profile of the five amphipods have been compared with other studied alternatives preys. Therefore, amphipods of ponds are good candidates to be used as feed, and are proposed as a new sustainable economic resource to use in aquaculture. G. insensibilis may be the best to be intensively cultured as an alternative feed resource because shows: 1) adequate PUFAS n-3 and PL composition;2) high levels of glycine, alanine, tyrosine, isoleucine and lysine; 3) high natural densities; 3 Pablo Jiménez-Prada 1,2* , Ismael Hachero-Cruzado 2 , Inmaculada Giraldez 3 , Catalina Fernández-4 Díaz 2 , César Vilas 2 , José P. Cañavate 2 , José M. Guerra-García 1 . 15 Coastal protection, nutrient cycling, erosion control, water purification, and carbon sequestration 16 are ecosystem services provided by salt marshes. Additionally, the salt ponds offers coastal 17 breeding and nursery habitat to fishes and invertebrates providing abundant amphipods 18 potentially useful as a resource in aquaculture. Fishmeal and fish oil are necessary food resources 19 to support aquaculture of carnivorous species due to their omega-3 long-chain polyunsaturated 20 fatty acids (n-3 LC-PUFA). Their dependence on limited fisheries constrains growth of 21 aquaculture and the need for n-3 LC-PUFA recommends the development of more sustainable 22 food sources. Due to their extensive feeding on detritus and primary producers, amphipods 23 appear as potential and high quality alternative feed res...