The role of pH, metal ions and their hydroxides in charge reversal of protein-coated nanoparticles

Schubert, Jonas; Radeke, Carmen; Fery, Andreas; Chanana, Munish

doi:10.1039/c8cp05946b

Cited by 36 publications

(26 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This shell connects the NPs and is also found in their immediate surroundings. As investigated by Schubert et al, 43 this shell can be attributed to the presence of Gd hydroxides and can be considered to be an argument for the aggregation of the nanoparticles via interactions of sulfonate groups with the Gd 3+ ions without replacement of the ligands from the rst coordination sphere of Gd. Together, reversibility of the interactions observed in QCM-D experiments and the possibility to reverse the particle aggregation by the addition of a strong coordinating ligand for the Gd 3+ ions, i.e.…”

Section: Origin Of Aggregation Of Aunp@bspp By Gd 3+mentioning

confidence: 88%

Easy colorimetric detection of gadolinium ions based on gold nanoparticles: key role of phosphine-sulfonate ligands

et al. 2020

View full text Add to dashboard Cite

show abstract

Section: Origin Of Aggregation Of Aunp@bspp By Gd 3+mentioning

confidence: 88%

Easy colorimetric detection of gadolinium ions based on gold nanoparticles: key role of phosphine-sulfonate ligands

et al. 2020

View full text Add to dashboard Cite

show abstract

“…One of the first challenges in the synthesis of NPs is to achieve good colloidal stability and uniformly dispersed samples. 51 Therefore, the stability of NPs was evaluated as a function of time. Size and surface charge variations usually engender NP instability, with aggregation and precipitation over time.…”

Section: Stability Of Nanoparticlesmentioning

confidence: 99%

Chitosan coated metallic nanoparticles with stability, antioxidant, and antibacterial properties: Potential for wound healing application

Sharifiaghdam

Shaabani

Asghari

et al. 2021

J of Applied Polymer Sci

View full text Add to dashboard Cite

Metal nanoparticles (NPs) are extensively adopted in the treatment of wounds owing to their proven antibacterial activity and enhanced wound healing effects. This study is aimed at providing a simple technique of synthesis and a comprehensive report from metal-based NPs including copper, silver, gold, and selenium for wound healing. It also constitutes their application in terms of stability, antibacterial and antioxidant effects as well as cellular interactions. In this study, gold (AuNPs@CS) and selenium (SeNPs@CS) NPs are introduced as highly stable NPs that can provide antioxidant efficacy more than other NPs to reduce ROS levels in H 2 O 2 -induced NIH-3T3 fibroblast cells. These NPs exhibited antibacterial effects in Gram-negative and Gram-positive bacteria, indicating their potential as proper antibiotics. Furthermore, our results revealed that these NPs can exert their antioxidant and antibacterial efficacy with even higher uptake in NIH-3T3 fibroblast cells for AuNPs@CS, or with the lowest uptake for SeNPs@CS. This provides an opportunity for the modification of NPs by altering their core and thus exert a different effect on the cells based on application. By enhancing the stability, antioxidant and antibacterial properties in AuNPs@Cs and SeNPs@Cs with the least toxicological risk, our concept opens new opportunities to design more efficient wound dressings.

show abstract

“…In addition to purely charge-mediated effects, cation-induced pH changes need to be considered as well. Schubert et al [455] studied the influence of metal ions and their hydroxides and the associated pH changes on NPs coated with BSA. This study reports both charge inversion of the NPs and a decrease of the solution pH upon addition of several trivalent cations, going along with the adsorption of metal hydroxide species on the NPs.…”

Section: Colloids and Nanoparticlesmentioning

confidence: 99%

“…This study reports both charge inversion of the NPs and a decrease of the solution pH upon addition of several trivalent cations, going along with the adsorption of metal hydroxide species on the NPs. Schubert et al [455] trace the charge inversion observed to these hydroxides, thus connecting pH and pure charge effects.…”

Section: Colloids and Nanoparticlesmentioning

confidence: 99%

Multivalent ions and biomolecules: Attempting a comprehensive perspective

2020

View full text Add to dashboard Cite

Ions are ubiquitous in nature. They play a key role for many biological processes on the molecular scale, from molecular interactions, to mechanical properties, to folding, to self‐organisation and assembly, to reaction equilibria, to signalling, to energy and material transport, to recognition etc. Going beyond monovalent ions to multivalent ions, the effects of the ions are frequently not only stronger (due to the obviously higher charge), but qualitatively different. A typical example is the process of binding of multivalent ions, such as Ca2+, to a macromolecule and the consequences of this ion binding such as compaction, collapse, potential charge inversion and precipitation of the macromolecule. Here we review these effects and phenomena induced by multivalent ions for biological (macro)molecules, from the “atomistic/molecular” local picture of (potentially specific) interactions to the more global picture of phase behaviour including, e. g., crystallisation, phase separation, oligomerisation etc. Rather than attempting an encyclopedic list of systems, we rather aim for an embracing discussion using typical case studies. We try to cover predominantly three main classes: proteins, nucleic acids, and amphiphilic molecules including interface effects. We do not cover in detail, but make some comparisons to, ion channels, colloidal systems, and synthetic polymers. While there are obvious differences in the behaviour of, and the relevance of multivalent ions for, the three main classes of systems, we also point out analogies. Our attempt of a comprehensive discussion is guided by the idea that there are not only important differences and specific phenomena with regard to the effects of multivalent ions on the main systems, but also important similarities. We hope to bridge physico‐chemical mechanisms, concepts of soft matter, and biological observations and connect the different communities further.

show abstract

The role of pH, metal ions and their hydroxides in charge reversal of protein-coated nanoparticles

Abstract: In this study, we investigated charge inversion of protein-coated Au nanoparticles caused by the addition of metal ions. Adsorbed metal hydroxides were identified to cause the charge inversion of the NPs by using a combination of cryo-TEM, EFTEM and ζ-potential measurements.

Cited by 36 publications

References 67 publications

Easy colorimetric detection of gadolinium ions based on gold nanoparticles: key role of phosphine-sulfonate ligands

Easy colorimetric detection of gadolinium ions based on gold nanoparticles: key role of phosphine-sulfonate ligands

Chitosan coated metallic nanoparticles with stability, antioxidant, and antibacterial properties: Potential for wound healing application

Multivalent ions and biomolecules: Attempting a comprehensive perspective

Contact Info

Product

Resources

About