The Role of Petrimonas mucosa ING2-E5AT in Mesophilic Biogas Reactor Systems as Deduced from Multiomics Analyses

Maus, Irena; Tubbesing, Tom Jonas; Wibberg, Daniel; Heyer, Robert; Hassa, Julia; Tomazetto, Geizecler; Huang, Liren; Bunk, Boyke; Spröer, Cathrin; Benndorf, Dirk; Zverlov, Vladimir V.; Pühler, Alfred; Klocke, Michael; Sczyrba, Alexander; Schlüter, Andreas

doi:10.3390/microorganisms8122024

Cited by 26 publications

(16 citation statements)

References 87 publications

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“…When analyzing the top 25 genera, only four genera were shared between WW and PF of which three were unclassified ( Supplementary Figure 4 ). A recent meta-omics study on the genus Petrimonas revealed its function in sugar and amino acid fermentation pathways and its widespread occurrence in biogas reactors, particularly in biogas plants with process disorders ( Maus et al, 2020 ). The dominance of this genus in the WW and PF communities might be related to microbial biomass turnover under stress conditions such as substrate shift in phase 1 and starvation in phases 2–5.…”

Section: Resultsmentioning

confidence: 99%

Microbial Communities in Flexible Biomethanation of Hydrogen Are Functionally Resilient Upon Starvation

et al. 2021

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Ex situ biomethanation allows the conversion of hydrogen produced from surplus electricity to methane. The flexibility of the process was recently demonstrated, yet it is unknown how intermittent hydrogen feeding impacts the functionality of the microbial communities. We investigated the effect of starvation events on the hydrogen consumption and methane production rates (MPRs) of two different methanogenic communities that were fed with hydrogen and carbon dioxide. Both communities showed functional resilience in terms of hydrogen consumption and MPRs upon starvation periods of up to 14 days. The origin of the inoculum, community structure and dominant methanogens were decisive for high gas conversion rates. Thus, pre-screening a well performing inoculum is essential to ensure the efficiency of biomethanation systems operating under flexible gas feeding regimes. Our results suggest that the type of the predominant hydrogenotrophic methanogen (here: Methanobacterium) is important for an efficient process. We also show that flexible biomethanation of hydrogen and carbon dioxide with complex microbiota is possible while avoiding the accumulation of acetate, which is relevant for practical implementation. In our study, the inoculum from an upflow anaerobic sludge blanket reactor treating wastewater from paper industry performed better compared to the inoculum from a plug flow reactor treating cow manure and corn silage. Therefore, the implementation of the power-to-gas concept in wastewater treatment plants of the paper industry, where biocatalytic biomass is readily available, may be a viable option to reduce the carbon footprint of the paper industry.

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Section: Resultsmentioning

confidence: 99%

Microbial Communities in Flexible Biomethanation of Hydrogen Are Functionally Resilient Upon Starvation

et al. 2021

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“…To determine the distribution and abundance of the phage genomes in different biogas communities, fragment recruitments were performed. To this end, the tool SparkHit [52] was used as described previously [53] and genome sequences of each MAVG were employed as a template. Corresponding computations were scaled-up and parallelized by using the de.NBI Cloud environment (https://www.denbi.de/cloud, accessed on 12 September 2021).…”

Section: Fragment Recruitmentmentioning

confidence: 99%

Phage Genome Diversity in a Biogas-Producing Microbiome Analyzed by Illumina and Nanopore GridION Sequencing

et al. 2022

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The microbial biogas network is complex and intertwined, and therefore relatively stable in its overall functionality. However, if key functional groups of microorganisms are affected by biotic or abiotic factors, the entire efficacy may be impaired. Bacteriophages are hypothesized to alter the steering process of the microbial network. In this study, an enriched fraction of virus-like particles was extracted from a mesophilic biogas reactor and sequenced on the Illumina MiSeq and Nanopore GridION sequencing platforms. Metagenome data analysis resulted in identifying 375 metagenome-assembled viral genomes (MAVGs). Two-thirds of the classified sequences were only assigned to the superkingdom Viruses and the remaining third to the family Siphoviridae, followed by Myoviridae, Podoviridae, Tectiviridae, and Inoviridae. The metavirome showed a close relationship to the phage genomes that infect members of the classes Clostridia and Bacilli. Using publicly available biogas metagenomic data, a fragment recruitment approach showed the widespread distribution of the MAVGs studied in other biogas microbiomes. In particular, phage sequences from mesophilic microbiomes were highly similar to the phage sequences of this study. Accordingly, the virus particle enrichment approach and metavirome sequencing provided additional genome sequence information for novel virome members, thus expanding the current knowledge of viral genetic diversity in biogas reactors.

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“…To identify additional MD1 T variant strains in biogas-producing microbial communities, metagenome fragment recruitments using the genome sequence of MD1 T as template were performed using SparkHit [45] as described previously [46]. Corresponding computations were scaled up and parallelized by using the de.NBI Cloud environment (www.denbi.de/cloud).…”

Section: Genome Featuresmentioning

confidence: 99%

Variimorphobacter saccharofermentans gen. nov., sp. nov., a new member of the family Lachnospiraceae, isolated from a maize-fed biogas fermenter

Rettenmaier

Thieme

Streubel

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Strain MD1T is an anaerobic, Gram-stain-negative bacterium isolated from a lab-scale biogas fermenter fed with maize silage. It has a rod-shaped morphology with peritrichously arranged appendages and forms long chains of cells and coccoid structures. The colonies of MD1T were white, circular, slightly convex and had a smooth rim. The isolate is mesophilic, displaying growth between 25 and 45 °C with an optimum at 40 °C. It grew at pH values of pH 6.7–8.2 (optimum, pH 7.1) and tolerated the addition of up to 1.5% (w/v) NaCl to the medium. The main cellular fatty acids of MD1T are C14:0 DMA and C16:0. Strain MD1T fermented xylose, arabinose, glucose, galactose, cellobiose, maltose, maltodextrin10, lactose starch, and xylan, producing mainly 2-propanol and acetic acid. The genome of the organism has a total length of 4163427 bp with a G+C content of 38.5 mol%. The two closest relatives to MD1T are Mobilitalea sibirica P3M-3T and Anaerotaenia torta FH052T with 96.44 or 95.8 % 16S rRNA gene sequence similarity and POCP values of 46.58 and 50.58%, respectively. As MD1T showed saccharolytic and xylanolytic properties, it may play an important role in the biogas fermentation process. Closely related variants of MD1T were also abundant in microbial communities involved in methanogenic fermentation. Based on morphological, phylogenetic and genomic data, the isolated strain can be considered as representing a novel genus in the family Lachnospiraceae , for which the name Variimorphobacter saccharofermentans gen. nov., sp. nov. (type strain MD1T=DSM 110715T=JCM 39125T) is proposed.

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The Role of Petrimonas mucosa ING2-E5AT in Mesophilic Biogas Reactor Systems as Deduced from Multiomics Analyses

Cited by 26 publications

References 87 publications

Microbial Communities in Flexible Biomethanation of Hydrogen Are Functionally Resilient Upon Starvation

Microbial Communities in Flexible Biomethanation of Hydrogen Are Functionally Resilient Upon Starvation

Phage Genome Diversity in a Biogas-Producing Microbiome Analyzed by Illumina and Nanopore GridION Sequencing

Variimorphobacter saccharofermentans gen. nov., sp. nov., a new member of the family Lachnospiraceae, isolated from a maize-fed biogas fermenter

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