The role of perfusion bioreactors in bone tissue engineering

Gaspar, Diana; Gomide, Viviane Silva; Monteiro, F.J.

doi:10.4161/biom.22170

Cited by 137 publications

(131 citation statements)

References 69 publications

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“…Consistently, dynamic compression enhances the vasculogenic activities of circulating endothelial precursors (EPCs) seeded in the demineralized bone matrix scaffolds (Kong et al, 2012). The relationship between mechanical inputs and resulting biological tissue structure, composition, and metabolism is reported and discussed in several other papers and reviews (David et al, 2008;Desmoulin et al, 2013;Gardel, Serra, Reis, & Gomes, 2014;Gaspar, Gomide, & Monteiro;S.-T. Li et al, 2014;van Griensven et al, 2009).…”

Section: The Need For Function: Mechano-electrical Stressmentioning

confidence: 81%

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

et al. 2015

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Bone plays several physiological functions and is the second most commonly transplanted tissue after blood. Since the treatment of large bone defects is still unsatisfactory, researchers have endeavoured to obtain scaffolds able to release growth and differentiation factors for mesenchimal stem cells, osteoblasts and endothelial cells in order to obtain faster mineralization and prompt a reliable vascularization.Nowadays, the application of osteoblastic cultures spans from cell physiology and pharmacology to cytocompatibility measurement and osteogenic potential evaluation of novel biomaterials. To overcome the simple traditional monocultures in vitro, co-cultures of osteogenic and vasculogenic precursors were introduced with very interesting results. Increasingly complex culture systems have been developed, where cells are seeded on proper scaffolds and stimulated so as to mimic the physiological conditions more accurately. These bioreactors aim at enabling bone regeneration by incorporating different cells types into bioinspired materials within a surveilled habitat.This review is focused on the most recent developments in the organomimetic cultures of osteoblasts and vascular endothelial cells for bone tissue engineering.

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Section: The Need For Function: Mechano-electrical Stressmentioning

confidence: 81%

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

et al. 2015

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“…Co-culture of MSCs along with endothelial cells in a hydrogel such as fibrin can also lead to the formation of vessel-like structures in what has been termed a 'prevascularized' tissue-engineered bone graft [105,106]. One difficulty for cell-seeded solid-cured polymer, ceramic or composite scaffolds or hydrogel scaffolds to gain entry in the clinic is that there is little experience with them in the clinic.…”

Section: Preculturing Strategies: Static Versus Perfusion Bioreactor mentioning

confidence: 99%

The Potential Impact of Bone Tissue Engineering in the Clinic

et al. 2016

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Bone tissue engineering (BTE) intends to restore structural support for movement and mineral homeostasis, and assist in hematopoiesis and the protective functions of bone in traumatic, degenerative, cancer, or congenital malformation. While much effort has been put into BTE, very little of this research has been translated to the clinic. In this review, we discuss current regenerative medicine and restorative strategies that utilize tissue engineering approaches to address bone defects within a clinical setting. These approaches involve the primary components of tissue engineering: cells, growth factors and biomaterials discussed briefly in light of their clinical relevance. This review also presents upcoming advanced approaches for BTE applications and suggests a probable workpath for translation from the laboratory to the clinic.

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“…When compared with spinner flask, on culturing rat MSCs, low secretion of osteocalcin than a spinner flask is observed. Though it solves a few of the limitation of static culture, but results from expression of osteoblastic markers are not adequate (Gasper, Gomide, & Monteiro, 2012).…”

Section: Rotating Wall Vesselsmentioning

confidence: 99%

Rapid Prototyping Assisted Scaffold Fabrication for Bone Tissue Regeneration

Sapkal¹,

Jaiswal²,

Kuthe³

2016

JMSR

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The review article focuses on Rapid Prototyped assisted scaffold fabrication for bone tissue regeneration, particularly in respect of its mechanical properties and cell culture abilities. The distinct feature of computer aided design and computer aided manufacturing (CAD & CAM), imaging technology and rapid prototyping (RP) technology has been used by different researchers to print porous scaffolds with requisite shape and interconnected channels for osseous tissue formation. This study concludes that the use of RP in scaffold manufacturing offers patient specific designed scaffolds with improved strength, in-vitro and in-vivo cell culture capability unlike traditional scaffold fabrication techniques. Tissue engineering using 3D Printing is a viable substitute for organ transplant, which requires willing donors to part with their organs. This study reviewed the benefits of RP/imaging/CAD-CAM to develop scaffolds for bone tissue regeneration and it serves those patients who could not be accurately treated by traditional means. The article is helpful to study the influence of RP in the field of organ transplant

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The role of perfusion bioreactors in bone tissue engineering

Cited by 137 publications

References 69 publications

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

The Potential Impact of Bone Tissue Engineering in the Clinic

Rapid Prototyping Assisted Scaffold Fabrication for Bone Tissue Regeneration

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