The Role of Ozone Carried by Liposomes in the Therapy of Infectious and Skin-Regenerating Ocular Surface

Cutarelli, Anna; Carlini, Gianpaolo; Sarno, Federica; Garofalo, Francesca; Altucci, Lucia; Corrado, Federica

doi:10.4236/jbise.2019.127026

Cited by 13 publications

(19 citation statements)

References 9 publications

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“…aureus and P . aeruginosa was observed by Cutarelli et al [ 18 ]. Serio et al [ 19 ] confirmed the antibacterial activity of ozonated sunflower seed oil (Oz.Or.Oil 30) against both Gram-negative ( E .…”

Section: Discussionmentioning

confidence: 92%

Ozone-Based Eye Drops Activity on Ocular Epithelial Cells and Potential Pathogens Infecting the Front of the Eye

et al. 2021

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Confirmation of the biological effectiveness of new ophthalmic preparations introduced in the market is an important element in maintaining the safety of using this type of medications. This study aimed to investigate the activity of Ozodrop® on human corneal and conjunctival epithelial cells, as well as its antibacterial and antifungal activity. Cytotoxicity analyses of ocular surface epithelial cells were performed in vitro by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) and Neutral Red uptake assays. The level of nitric oxide released by the cells was assessed by the Griess method. The reduction of the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical by the tested formulation was analyzed. Microbiological tests were also performed. It was found that the Ozodrop® preparation exhibited biological activity, but was less active than the reference antibiotics and the anti-yeast agent. The cytotoxic activity of the Ozodrop® formulation was dependent on the time of cell exposure to it. No toxic effect was observed in the short-term, for up to 3 h. It appeared after 24 h of exposure of the cells to the preparation. The drops showed antioxidant activity in the specified concentration range. They also stimulated the release of nitric oxide, mainly by corneal epithelial cells. The Ozodrop® formulation exhibits biological activity that can be considered useful in the treatment of infections in the front part of the eye.

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Section: Discussionmentioning

confidence: 92%

Ozone-Based Eye Drops Activity on Ocular Epithelial Cells and Potential Pathogens Infecting the Front of the Eye

et al. 2021

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“…More recently, the cytotoxicity of liposome-vehiculated ozonated oil against S. aureus and P. aeruginosa, which are among the most frequent eye pathogens, has been analyzed [100]. The microbiological results clearly show antimicrobial efficacy and there was no evidence of any toxicity against keratinocytes.…”

Section: Macro- Micro- and Nanoencapsulation Of Oilsmentioning

confidence: 99%

Ozonated Oils as Antimicrobial Systems in Topical Applications. Their Characterization, Current Applications, and Advances in Improved Delivery Techniques

Ugazio

Tullio²,

Binello

et al. 2020

Molecules

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The search for a wide spectrum of antimicrobial agents that can avoid resistance while maintaining reasonable side effects has led to ozonated oils experiencing an increase in scientific interest and clinical applications. The treatment of vegetable oils with ozone leads to the creation of a reservoir of ozone that slowly releases into the skin thanks to the fact that ozone can be held as ozonides of unsaturated fatty acids. Interest in the use of ozonated oils has meant that several ozonated-vegetable-oil-containing products have been commercialized as cosmetic and pharmaceutical agents, and in innovative textile products with antibacterial activity. New approaches to the delivery of ozonated oils have very recently appeared in an attempt to improve their characteristics and reduce drawbacks, such as an unpleasant odor, high viscosity and undesired effects on skin, including irritation and rashes. The present review focuses on the current status of delivery agents that use ozonated oils as antimicrobial agents in topical (dermal, skin, and soft tissues) treatments. Challenges and future opportunities for these delivery systems will also be discussed.

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“…The introduction of oxidizing agents in drops for disinfection of the ocular surface is very recent. Their use has been advocated on the basis of efficacy against all microorganisms, as well as the lack of induction of antibiotic resistance [13]. The ozonated…”

Section: Introductionmentioning

confidence: 99%

Bactericidal activity of three different antiseptic ophthalmic preparations as surgical prophylaxis

Tognetto

Pastore

Guerin

et al. 2021

Graefes Arch Clin Exp Ophthalmol

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Purpose In the era of antibiotic resistance, there is an increased interest in antiseptic solutions that might represent a reliable option for ocular surface disinfection. The objective of this study is to compare for the first time three different antiseptic ophthalmic preparations to assess their in vitro antimicrobial activity. Methods The antiseptic activity of three commercial ophthalmic solutions, IODIM (povidone-iodine 0.6% in hyaluronic acid vehicle—Medivis, Catania, Italy), OZODROP (nanoemulsion with ozonated oil—concentration not specified—FBVision, Ophthalmic Pharmaceuticals, Rome, Italy), and DROPSEPT (chlorhexidine 0.02% and vitamin E 0.5% Tocopherol Polyethylene Glycol 1000 Succinate—TPGS, Sooft Italia, Montegiorgio, Italy), was tested in vitro on six reference strains by time-killing assays. Viable cells were evaluated after 1, 15, 30 min; 2, 6, and 24 h exposure by seeding 100 µl of the suspension (or appropriate dilutions) on LB agar or Sabouraud-dextrose agar. All plates were incubated at 37 °C for 24 h and evaluated by manually counting the colonies. Results IODIM solution showed a very rapid microbicidal activity: the number of viable cells for all the tested strains was under the detection limit (less than 10 CFU/ml) already after 1 min exposure, and this result was maintained at every incubation time. The rapid antimicrobial activity of povidone-iodine was not replicated when testing the other two antiseptics. Conclusions The study reports the great efficacy in reducing bacterial load in a very short time of povidone-iodine 0.6% compared with other antiseptic preparations.

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The Role of Ozone Carried by Liposomes in the Therapy of Infectious and Skin-Regenerating Ocular Surface

Cited by 13 publications

References 9 publications

Ozone-Based Eye Drops Activity on Ocular Epithelial Cells and Potential Pathogens Infecting the Front of the Eye

Ozone-Based Eye Drops Activity on Ocular Epithelial Cells and Potential Pathogens Infecting the Front of the Eye

Ozonated Oils as Antimicrobial Systems in Topical Applications. Their Characterization, Current Applications, and Advances in Improved Delivery Techniques

Bactericidal activity of three different antiseptic ophthalmic preparations as surgical prophylaxis

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