The role of nitric oxide in testicular ischemia-reperfusion injury

Özokutan, Bülent Hayrı; Küçükaydın, Mustafa; Muhtaroğlu, Sabahattin; Tekın, Yeşim Bayoğlu

doi:10.1016/s0022-3468(00)80023-8

Cited by 36 publications

(18 citation statements)

References 21 publications

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“…In the testis, constitutive NOS was strongly detected in the interstitial cells, while in the epididymis both eNOS and nNOS expression were detected mainly in sperms and epithelium. Although it has been reported that iNOS is overexpressed in ischemia-reperfusion injuries of the testis [18], this is the first report of iNOS expression in the interstitial cells of the testis, as well as in basal cells and sperm in the epididymis of the normal horse.…”

Section: Discussionmentioning

confidence: 66%

“…On the other hand, excessive NOS may induce the production of large amounts of NO metabolites in response to a variety of stressors, possibly reducing the survival rate and motility of sperm cells [18,20]. High levels of NO may also reduce testosterone secretion, as indicated by the suppression of testosterone secretion in male rats treated with the NO donor, isosorbide dinitrate (ISDN) [1].…”

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confidence: 99%

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Expression of Constitutive Endothelial, Neuronal and Inducible Nitric Oxide Synthase in the Testis and Epididymis of Horse

Ha¹,

Kim

Shin

2004

The Journal of Veterinary Medical Science

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ABSTRACT. The expression of three isoforms of nitric oxide synthase (NOS) were examined in the testis and epididymis of a thoroughbred horse. Immunohistochemical studies demonstrated the presence of eNOS immunostaining in some germ cells in the seminiferous tubules and in vascular endothelial cells in the interstitial tissues. Interstitial cells, most likely Leydig cells, were also intensel y immunopositive for eNOS. The pattern of immunostaining for nNOS was similar to that for eNOS in the testis. Weak expression of iNOS was detec ted in the seminiferous tubules of the testis, but intense expression was found in interstitial cells. Inducible NOS was also stron gly detected in stereocilia, sperm, epithelium and connective tissue of the epididymis of normal horses. These findings suggest that three isoforms of NOS are expressed in the testis and epididymis of horse and that they play important roles in the biology of interstitial cells that produce testosterone, as well as in spermatogenesis in the seminiferous tubules. KEY WORDS: epididymis, equine, nitric oxide synthase, testis.

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Section: Discussionmentioning

confidence: 66%

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confidence: 99%

Expression of Constitutive Endothelial, Neuronal and Inducible Nitric Oxide Synthase in the Testis and Epididymis of Horse

Ha¹,

Kim

Shin

2004

The Journal of Veterinary Medical Science

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“…This leads us to seek pharmacological agents that can reduce VCL-induced injuries. Antioxidants have been used successfully to reduce oxidative stress related injuries in many organs including the testis [Suziki and Sofikitis 1999;Ozokutan et al 2000].…”

Section: Introductionmentioning

confidence: 99%

Silymarin protects from varicocele-induced damages in testis and improves sperm quality: evidence for E2f1 involvement

Moshtaghion¹,

Malekinejad²,

Razi

et al. 2013

Systems Biology in Reproductive Medicine

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This study was designed to evaluate the protective effect of silymarin (SMN) on varicocele-induced damage in testis and its effects on sperm parameters and on antioxidant status. Wistar rats were divided into three groups: control-sham, varicocele-induced, and SMN-treated varicocelized (50mg/kg, orally) rats. The sperm count, DNA integrity, and histone-protamine transition was evaluated after 42 days. The antioxidant status was analyzed by determining testicular malondialdehyde (MDA) and total thiol molecules (TTM). The endocrine status of the testicular tissue was estimated by counting the normal Leydig cell distribution/mm 2 and by determination of serum testosterone. The expression of E2f1 mRNA was analyzed using RT-PCR. Carbohydrate depletion and lipid foci replacement in germinal cells were examined by histochemical analyses. Silymarin rehabilitated the varicocele-induced Leydig cell degeneration and testosterone reduction. In addition, SMN recovered the varicocele-induced reduction of TTM and lowered significantly (P < 0.05) the varicocele-elevated content of MDA. The SMN treatment resulted in a significant (P < 0.05) down-regulation of the VCL-up-regulated E2f1 mRNA. Silymarin-treated animals were protected from varicoceleinduced testicular atrophy and these animals showed a significant (P < 0.05) increase in the percentage of seminiferous tubules with positive tubular differentiation, repopulation, and spermiogenesis indices. Furthermore, SMN improved the varicocele-induced carbohydrate reduction in germinal cells. Our data suggest that in addition to oxidative stress, alteration in the testicular endocrine function plays a crucial role in the pathogenesis of varicocele. Moreover, the protective effects of SMN on varicocele-induced damage may reflect its antioxidant property, which may be mediated via the E2f1 transcription factor.

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“…12 However, Ozokutan et al found increased damage with Arg after four hours of torsion/detorsion in a rat model. 29 The PDE5 inhibitors were originally developed for treatment of erectile dysfunction and today these molecules can be used in other diseases, such as benign prostate hyperplasia and pulmonary hypertension. 13,30,31 The effect of PDE5 inhibitors on testicular torsion is conflicting in the literature.…”

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confidence: 99%

The protective effect of L-arginine, tadalafil, and their combination in rat testes after ischemia and reperfusion injury

Ozmerdiven

Çoşkun

Kaygısız

et al. 2017

CUAJ

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Cite as: Can Urol Assoc J 2017;11(1-2):E19-25. http://dx.doi.org/10.5489/cuaj.3872 Published online January 12, 2017 AbstractIntroduction: Nitric oxide (NO) plays an important role in the ischemia and reperfusion process. In this study, we aimed to examine the effect of L-arginine, tadalafil, and their combination for prevention of the ischemia reperfusion injury after testis torsion in rats. Methods: A total of 40 adult, male Sprague-Dawley rats were allocated into five groups. Three hours of left testicular torsion was performed in each group, excluding the control group. While the ischemia reperfusion (I/R) group had no treatment, I/R + Arg group received L-arginine, I/R + Td group received tadalafil and I/R + Arg + Td group received tadalafil and L-arginine 30 minutes before the detorsion. Then the left testis was untwisted for four hours of reperfusion. After bilateral orchiectomy, lipid peroxidation (LPx) and glutathione (GSH) activities were examined in testicular tissue. Spermatogenesis was evaluated with Johnsen's score. Results: LPx levels of the I/R group were found to be significantly higher than for groups that received drugs for both testes (p<0.001). GSH levels of the combination group were higher than I/R group in ipsilateral testis (p<0.01) and it was significantly higher than other groups for contralateral testis (p<0.001 for I/R group, p<0.01 for I/R + Arg, p<0.05 for I/R + Td). Mean Johnsen's score of the I/R group was found to be significantly lower than treatment groups in ipsilateral testis (p<0.001 for I/R + Arg + Td group, p<0.01 for other treatment goups) and contralateral testis (p<0.001). The mean Johnsen score of the combination group was significantly higher than that of other treatment groups in ipsilateral testis (p<0.05) and it was significantly higher than in the I/R + Td group in the contralateral testis (p<0.05). Conclusions: L-arginine, tadalafil, and combination of these two molecules showed protective effect against ischemia/reperfusion injury for both testes after unilateral testis torsion.

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The role of nitric oxide in testicular ischemia-reperfusion injury

Cited by 36 publications

References 21 publications

Expression of Constitutive Endothelial, Neuronal and Inducible Nitric Oxide Synthase in the Testis and Epididymis of Horse

Expression of Constitutive Endothelial, Neuronal and Inducible Nitric Oxide Synthase in the Testis and Epididymis of Horse

Silymarin protects from varicocele-induced damages in testis and improves sperm quality: evidence for E2f1 involvement

The protective effect of L-arginine, tadalafil, and their combination in rat testes after ischemia and reperfusion injury

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