The role of mitochondrial DNA alterations in esophageal squamous cell carcinomas

Lin, Ching-Heng; Chang, Shi Chuan; Wang, Liang Shun; Chou, Teh Ying; Hsu, Wen Hu; Wu, Yu Chung; Wei, Yau‐Huei

doi:10.1016/j.jtcvs.2009.04.007

Cited by 87 publications

(103 citation statements)

References 21 publications

(43 reference statements)

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“…These tissue samples were stored in 1.5-ml Eppendorf vials and mixed with 500 µl xylene (Merck KGaA, Darmstadt, Germany) at room temperature for 16 h. After centrifuging at 10,000 x g for 10 min at room temperature and discarding the supernatants, these tissue samples were re-hydrated with 100, 80, 60 and 40% alcohol aqueous solution and then pure distilled water for 5 min in steps. Finally, the hydrated tissue samples were mixed with 200 µl QuickExtract DNA extraction solution (Epicenter, Madison, WI, uSA) plus 3 µl of 5% butylated hydroxytoluene in methanol to extract total cellular DNA at 65˚C for 3 h as previously described (19,20). The DNA sample was kept at -20˚C until use.…”

Section: Patient Selection Tissue Preparation and Dna Extractionmentioning

confidence: 99%

“…The sequences of primers used for nDNA amplification (18S rRNA region) were: 18SF1546, 5'-T AGAGGGACAAGTGGCGTTC-3'; and 18SR1650, 5'-CGCT GAGCCAGTCAGTGT-3' (21). The equations of standard curves set for mtDNA and nDNA quantification were set as previously described (19,20,22). Then the mtDNA and nDNA copies of the clinical samples relative to mtDNA and nDNA copies of the 143B osteosarcoma cells were calculated.…”

Section: Standard Curves For Mtdna and Ndna Quantificationsmentioning

confidence: 99%

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Mitochondrial DNA alterations correlate with the pathological status and the immunological ER, PR, HER-2/neu, p53 and Ki-67 expression in breast invasive ductal carcinoma

Lin¹,

Chang²,

Ou³

et al. 2015

Oncology Reports

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Abstract. We analyzed the changes in mitochondrial DNA (mtDNA) copy numbers and the shifting of mtDNA D310 sequence variations (D310 mutation) with their relationships to pathological status and the expression levels of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor-2 (HER-2/neu), tumor-suppressor protein p53 and cellular proliferation protein Ki-67 in breast invasive ductal carcinoma (BIDC), respectively. Fifty-one paraffin-embedded BIDCs and their paired non-cancerous breast tissues were dissected for DNA extraction. The mtDNA copy number and mtDNA D310 sequence variations were determined by quantitative real-time polymerase chain reaction (q-PCR) and PCR-based direct sequencing, respectively. The expression levels of ER, PR, HER-2/neu, p53 and Ki-67 were determined by immunohistochemical (IHC) staining. Compared to the paired non-cancerous breast tissues, 24 (47.1%) BIDCs had elevated mtDNA copy numbers and 29 (56.9%) harbored mtDNA D310 mutations. Advanced T-status (p=0.056), negative-ER (p=0.005), negative-PR (p=0.007), positive-p53 (p=0.050) and higher Ki-67 (p=0.004) expressions were related to a higher mtDNA copy ratio. In addition, advanced T-status (p=0.019) and negative-HER-2/neu expression (p=0.061) were associated with mtDNA D310 mutations. In conclusion, higher mtDNA copy ratio and D310 mutations may be relevant biomarkers correlated with pathological T-status and the expression levels of ER, PR, HER-2/neu, p53 and Ki-67 in BIDCs.

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Section: Patient Selection Tissue Preparation and Dna Extractionmentioning

confidence: 99%

Section: Standard Curves For Mtdna and Ndna Quantificationsmentioning

confidence: 99%

Mitochondrial DNA alterations correlate with the pathological status and the immunological ER, PR, HER-2/neu, p53 and Ki-67 expression in breast invasive ductal carcinoma

Lin¹,

Chang²,

Ou³

et al. 2015

Oncology Reports

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“…Numerous reports have indicated that the copy number of mtDNA changes in various types of cancer (6)(7)(8)(9)(10)(11)17). In 1989, Yamamoto et al first reported the increased mitochondrial genomic expression in pre-malignant lesions of the colon in patients with pre-cancerous familial polyposis coli (18).…”

Section: A B Cmentioning

confidence: 99%

“…The copy number is reduced in hepatocellular and renal cell carcinomas and breast cancer (6)(7)(8). However, it is increased in head and neck and ovarian cancers and esophageal squamous cell carcinomas (9)(10)(11). However, whether and and the manner in which mtDNA copy number changes in colorectal cancer remains to be determined.…”

Section: Introductionmentioning

confidence: 99%

Correlation between increased copy number of mitochondrial DNA and clinicopathological stage in colorectal cancer

Yang¹

2011

Oncol Lett

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Abstract. Change of mitochondrial DNA (mtDNA) copy numbers is considered to be an important hallmark of cancers. However, whether quantitative changes in mtDNA occur during the initiation and progression of colorectal cancer remains to be determined. Quantitative change in mtDNA was observed during the initiation and progression of colorectal cancer and possible correlations of the mtDNA copy number in colorectal cancer with the clinicopathological stage were investigated. Tumor tissues and the corresponding noncancerous tissues were surgically resected from 24 colon and 20 rectal patients between 2008 and 2009. β-actin expression was quantified in all of the specimens, and the copy numbers were calculated. In colorectal cancer, the quantitative changes of mtDNA exhibited a significant increase. In 24 cases of colon cancer, the average relative mtDNA copy number ratios were 115.15±31.57 in cancer tissues and 54.09±13.22 in the corresponding non-cancerous tissues (p<0.01). Furthermore, in 20 cases of rectal cancer, the ratios were 145.6±43.83 in cancer tissues and 55.58±12.47 in the corresponding non-cancerous tissues (p<0.001). Following correlation with clinicopathological data, change of the mtDNA copy number in colorectal cancer exhibited a significant association with clinicopathological stage, but no association with gender. Moreover, this increase was particularly marked in stages Ⅰ and Ⅱ. Our results indicate that mtDNA copy number plays a significant role during the initiation and progression of colorectal cancer, particularly during early clinicopathological stages.

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“…이 결과를 Bionics Inc. (Seoul, Korea)에 의 뢰하여 ABI 3730 DNA sequencer를 이용하여 직접 염기 서열분석을 시행하여 결과를 분석하여 D310과 D514의 다형성을 확인하였다. 미토콘드리아의 복제수는 real-time PCR의 방법으로 β-actin을 이용하여 input DNA의 양을 표준화하여 측정 하였다 [13,17]. 이가 종양에서 높게 나타나는 것을 보고하였다 [19].…”

Section: 재료 및 방법unclassified

Association between Mitochondrial D-loop Polymorphism and Copy Number

Lee

Kim

2014

Korean J Phys Anthropol

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The role of mitochondrial DNA alterations in esophageal squamous cell carcinomas

Cited by 87 publications

References 21 publications

Mitochondrial DNA alterations correlate with the pathological status and the immunological ER, PR, HER-2/neu, p53 and Ki-67 expression in breast invasive ductal carcinoma

Mitochondrial DNA alterations correlate with the pathological status and the immunological ER, PR, HER-2/neu, p53 and Ki-67 expression in breast invasive ductal carcinoma

Correlation between increased copy number of mitochondrial DNA and clinicopathological stage in colorectal cancer

Association between Mitochondrial D-loop Polymorphism and Copy Number

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